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Detection of messenger RNA from the isoleucine-valine operons of Salmonella typhimurium by heterologous DNA-RNA hybridization: Involvement of transfer RNA in transcriptional repression

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Summary

A hybridization assay using Escherichia coli K-12 DNA isolated from the specialized transducing bacteriophage λCI857St68h80 dilv was used to examine the rate of synthesis of the messenger RNA's (mRNA) derived from the isoleucine-valine (ilv) gene cluster of Salmonella typhimurium. In all cases examined, changes in ilv enzyme levels could be correlated with changes in the rate of synthesis of ilv mRNA. Several well characterized regulatory mutants of S. typhimurium had rates of synthesis of ilv mRNA 3 to 8-fold higher than the repressed wildtype strain. The increased rates of ilv mRNA synthesis found in a hisT strain as well as in isoleucyl-and leucyl-tRNA synthetase mutants, strongly suggests a role for branched-chain aminoacyl-tRNA's in transcriptional control.

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Author notes

  1. Geoffrey Childs

    Present address: Department of Medicine, Stanford University, School of Medicine, 94304, Palo Alto, California, U.S.A.

Authors and Affiliations

  1. Department of Biochemistry, State University of New York, 11794, Stony Brook, New York, U.S.A.

    Geoffrey Childs, Frank Sonnenberg & Martin Freundlich

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  1. Geoffrey Childs
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  2. Frank Sonnenberg
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  3. Martin Freundlich
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Communicated by W. Arber

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Childs, G., Sonnenberg, F. & Freundlich, M. Detection of messenger RNA from the isoleucine-valine operons of Salmonella typhimurium by heterologous DNA-RNA hybridization: Involvement of transfer RNA in transcriptional repression. Molec. gen. Genet. 151, 121–126 (1977). https://doi.org/10.1007/BF00338686

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  • DOI: https://doi.org/10.1007/BF00338686

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