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A new gene in E. coli RNA synthesis

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Summary

A novel spontaneous temperature sensitive mutant of Escherichia coli, which stops synthesizing stable RNA and some proteins immediately upon temperature shift from 30° C to 42° C, is described. Stable RNA species are not preferentially degraded in the mutant at the nonpermissive temperature. The guanine polyphosphate compounds, ppGpp (MS1) and pppGpp (MS2), are not produced at 42° C. The mutant strain does not grow at 42° C in either broth or defined minimal medium supplemented with any of a variety of carbon sources. The temperature sensitive mutation in this strain maps between dap A, E and pts I and defines a new locus affecting RNA synthesis in E. coli.

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Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut, USA

    Hope H. Liebke

  2. Genetics and Cell Biology Section, Biological Sciences Group, The University of Connecticut, 06268, Storrs, Connecticut, USA

    Joseph F. Speyer

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  1. Hope H. Liebke
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  2. Joseph F. Speyer
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Communicated by E. Bautz

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Liebke, H.H., Speyer, J.F. A new gene in E. coli RNA synthesis. Molec Gen Genet 189, 314–320 (1983). https://doi.org/10.1007/BF00337823

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  • DOI: https://doi.org/10.1007/BF00337823

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