Summary
The sciatic nerve of the rat was constricted at two sites about 10 mm apart. Three and seventeen hours later, a part of the nerve including the intermediate segment between the two crushed regions was dissected out and studied electron microscopically. Mitochondria and other organelles accumulated at the ends of interrupted fibres both proximally and distally of either crushed region. In the nerve segment between the two constrictions mitochondria collected at the fibre ends, whereas their number became significantly reduced in axons of the middle part of the segment. It is inferred that this translocation of mitochondria is brought about by a bidirectional shift of particles towards the fibre ends at which they become deposited.
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References
Burdwood, W. O.: Rapid bidirectional particle movements in neurons. J. Cell Biol. 27, 115A (1965).
Dahlström, A.: The effect of reserpine and tetrabenazine on the accumulation of noradrenaline in the rat sciatic nerve after ligation. Acta physiol. scand. 62, 167–179 (1967).
—, and J. Häggendal: Studies on the transport and life-span of amine storage granules in a peripheral adrenergic neuron system. Acta physiol. scand. 67, 278–288 (1966).
Estable, C., Acosta-Ferreira, W., and J. R. Sotelo: An electron microscope study of the regenerating nerve fibres. Z. Zellforsch. 46, 387–399 (1957).
Friede, R. L.: Electrophoretic production of reactive axon swellings in vitro and their histochemical properties. Acta neuropath. (Berl.) 3, 217–228 (1964).
—: Axon swellings produced in vivo in isolated segments of nerves. Acta neuropath. (Berl.) 3, 229–237 (1964).
Holtzman, E., and A. B. Novikoff: Lysosomes in the rat sciatic nerve following crush. J. Cell Biol. 27, 651–669 (1965).
Hughes, A.: The growth of embryonic neurites. A study on cultures of chick neural tissues. J. Anat. (Lond.) 87, 150–162 (1953).
Kapeller, K., and D. Mayor: The accumulation of noradrenaline in constricted sympathetic nerves as studied by fluorescence and electron microscopy. Proc. roy. Soc. B 167, 282–292 (1967).
—: Accumulation of organelles distal to the site of constriction of post-ganglionic sympathetic nerves. J. Physiol. (Lond.) 194, 95–96P (1968).
Kerkut, G. A., A. Shapira, and R. J. Walker: The transport of labelled material from CNS ⇌ muscle along a nerve trunk. Comp. Biochem. Physiol. 23, 729–748 (1967).
Lasek, R. J.: Bidirectional transport of radioactively labelled axoplasmic components. Nature (Lond.) 216, 1212–1214 (1967).
Lubińska, L.: Axoplasmic streaming in regenerating and in normal nerve fibres. In: Progress in brain research, ed. M. Singer and J. P. Schadé, vol. 13, p. 1–71. Amsterdam: Elsevier 1964.
—, S. Niemierko, B. Oderfeld-Nowak, and L. Schwarc: Behaviour of acetylcholinesterase in isolated nerve segments. J. Neurochem. 11, 493–513 (1964).
—, B. Oderfeld, L. Schwarc, and J. Zelená: Bidirectional movements of axoplasm in peripheral nerve fibers. Acta Biol. exp. (Warszawa) 23, 239–247 (1963).
Mayor, D., and K. Kapeller: Fluorescence microscopy and electron microscopy of adrenergic nerves after constriction at two points. J. roy. micr. Soc. 87, 277–294 (1967).
Miani, N.: Proximo-distal movement of phospholipid in the axoplasm of the intact and regenerating neurons. In: Progress in brain research, ed. M. Singer and J. P. Schadé, vol. 13, p. 115–126. Amsterdam: Elsevier 1964.
Nakai, J.: Dissociated dorsal root ganglia in tissue culture. Amer. J. Anat. 99, 81–129 (1956).
Niemierko, S., and L. Lubińska: Two fractions of axonal acetylcholinesterase exhibiting different behaviour in severed nerves. J. Neurochem. 14, 761–769 (1967).
Pellegrino de Iraldi, A., and E. de Robertis: The neurotubular system of the axon and the origin of granulated and non-granulated tubules in regenerating nerves. Z. Zellforsch. 87, 330–344 (1968).
Pomerat, C. M.: Cinematology, indispensable tool for cytology. Int. Rev. Cytol. 11, 307–339 (1961).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Sabatini, D. D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Scharf, J. H., u. R. Blume: Über die Abhängigkeit der axonalen Mitochondrienzahl vom Kaliber der segmentierten Nervenfaser auf Grund einer Regressionsanalyse. J. Hirnforsch. 6, 361–376 (1964).
Schlote, W.: Zur Abgrenzung reaktiver von regenerativen Vorgängen im Axoplasma zentraler Nervenfasern. Verh. Dtsch. Ges. Pathol. 50. Tagg. Heidelberg, S. 277–280 (1966a).
—: Der Aufbau von Schichtenkörpern im Axoplasma durchtrennter Opticusfasern distal der Läsion. J. Ultrastruct. Res. 16, 548–568 (1966b).
—, u. H. Hager: Elektronenmikroskopische Befunde zur Feinstruktur von Axonveränderungen im peritraumatischen Bereich nach experimenteller Strangdurchtrennung am Rückenmark der weißen Ratte. Naturwissenschaften 47, 448–451 (1960).
Speidel, C. C.: Studies of living nerves. I. The movements of individual sheath cells and nerve sprouts correlated with the process of myelin sheath formation in amphibian larvae. J. exp. Zool. 61, 279–317 (1932).
—: Studies of living nerves. II. Activities of ameboid growth cones, sheath cells, and myelin segments, as revealed by prolonged observation of individual nerve fibres in frog tadpoles. Amer. J. Anat. 52, 1–79 (1933).
—: Studies on living nerves. III. Phenomena of nerve irritation, recovery, degeneration and repair. J. comp. Neurol. 61, 1–82 (1935a).
—: Studies of living nerves. IV. Growth, regeneration, and myelination of the peripheral nerves in salamanders. Biol. Bull. 68, 142–163 (1935b).
Watson, W. E.: Centripetal passage of labelled molecules along mammalian motor axons. J. Physiol. (Lond.) 196, 122–123P (1968).
Webster, H. de F.: Transient, focal accumulation of axonal mitochondria during the early stages of Wallerian degeneration. J. Cell Biol. 12, 361–384 (1962).
Wechsler, W., u. H. Hager: Elektronenmikroskopische Befunde zur Feinstruktur von Axonveränderungen in regenerierenden Nervenfasern des Nervus ischiadicus der weißen Ratte. Acta neuropath. (Berl.) 1, 489–506 (1962).
Weiss, P.: Neuronal dynamics. Neurosci. Res. Prog. Bull. 5, 371–400 (1967).
—, and H. B. Hiscoe: Experiments on the mechanism of nerve growth. J. exp. Zool. 107, 315–395 (1948).
—, and A. Pillai: Convection and fate of mitochondria in nerve fibers: axonal flow as vehicle. Proc. nat. Acad. Sci. (Wash.) 54, 48–56 (1965).
—, A. C. Taylor, and A. Pillai: The nerve fiber as a system in continuous flow: microcine-matographic and electronmicroscopic demonstrations. Science 136, 330 (1962).
Wettstein, R., and J. R. Sotelo: Electron microscope study on the regenerative process of peripheral nerves of mice. Z. Zellforsch. 59, 708–730 (1963).
Zeleá, J., and E. Gutmann: Bidirectional shifting of mitochondria along axons. [In Czech.] Čs. Fysiol. 17, 39–40 (1968a).
- -Accumulation of organelles in central and peripheral stumps of interrupted axons. In: Macromolecules and the function of the neuron, ed. Z. Lodin. Excerpta Medica Monograph Series, p. 140–152, Amsterdam 1968b.
—, and Lubińska: Early changes of acetylcholinesterase activity near the lesion in crushed nerves. Physiol. bohemoslov. 11, 261–268 (1962).
—, and E. Gutmann: Accumulation of organelles at the ends of interrupted axons. Z. Zellforsch. 91, 200–219 (1968).
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Skillful technical assistance of Mrs. M. Sobotková and Mr. M. Doubek is gratefully acknowledged.
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Zelená, J. Bidirectional movements of mitochondria along axons of an isolated nerve segment. Z. Zellforsch. 92, 186–196 (1968). https://doi.org/10.1007/BF00335646
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DOI: https://doi.org/10.1007/BF00335646