Summary
With the lac operon fusion technique, mutants were isolated in two genes that specify two outer membrane proteins designated FhuE (76 K) and Fiu (83 K). The synthesis of both proteins was increased under low iron growth conditions. The FhuE-protein was shown to be necessary for iron uptake via coprogen, an iron chelator produced by certain fungi, e.g. Neurospora crassa. In addition to fhueE the genes fhuCDB, tonB and exbB were necessary for iron coprogen uptake. The gene fhuE was mapped between kdp and gltA near 16 min on the genetic map of E. coli K12, while gene fiu was mapped near 18 min between chlA and chlE. Nor iron transport system could be assigned as yet to the Fiu protein.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44:1–56
Braun V (1983) The iron transport systems of Escherichia coli. In: Martonosi AN (ed) The enzymes of biological membranes, vol 3. Plenum Press, New York
Braun V, Burkhardt R, Schneider R, Zimmermann L (1982) Chromosomal genes for ColV plasmid-determined iron(III)-aerobactin transport in Escherichia coli. J Bacteriol 151:553–559
Calos MP, Miller JH (1980) Transposable elements. Cell 20:579–595
Casadaban MJ, Cohen SN (1979) Lactose genes fused to exogenous promoters in one step using a Mu lac bacteriophage: In vivo probe for transcriptional control sequences. Proc Natl Acad Sci USA 76:4530–4533
Davies JK, Reeves P (1975) Genetics of resistance to colicins in Escherichia coli K-12: Cross resistance among colicins of group B. J Bacteriol 123:96–101
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Epstein W, Davies M (1970) Potassium-dependent mutants of Escherichia coli K-12. J Bacteriol 101:836–843
Fredericq P, Smarda J (1970) Complexite du facteur colicinogene B. Ann Inst Pasteur 118:767–774
Hantke K (1981) Regulation of ferric iron transport in Escherichia coli K-12: Isolation of a constitutive mutant. Mol Gen Genet 182:288–292
Hantke K (1982) Negative control of iron uptake systems in Escherichia coli. FEMS Microbiol Lett 15:83–86
Hantke K, Braun V (1978) Functional interaction of the tonA/tonB receptor system in Escherichia coli. J Bacteriol 135:190–197
Hantke K, Zimmermann L (1981) The importance of the exbB gene for vitamin B12 and ferric iron transport. FEMS Microbiol Lett 12:31–35
Keller-Schierlein W, Diekmann H (1970) Zur Konstitution des Coprogens. Helv Chim Acta 53:2035–2044
Luckey M, Pollack JR, Wayne R, Ames BN, Neilands JB (1972) Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers. J Bacteriol 111:731–738
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Neilands JB (1981) Microbial iron compounds. Annu Rev Biochem 50:715–731
Pascal MC, Burini JF, Ratouchniak J, Chippaux M (1982) Regulation of the nitrate reductase operon: effect of mutations in chlA,B,D and E genes. Mol Gen Genet 188:103–106
Plastow GS, Holland IB (1979) Identification of an Escherichia coli inner membrane polypeptide specified by a λ-tonB transducing bacteriophage. Biochem Biophys Res Commun 90:1007–1014
Stewart V, MacGregor CH (1982) Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE and chlG loci. J Bacteriol 151:788–799
Author information
Authors and Affiliations
Additional information
Communicated by A. Böck
Rights and permissions
About this article
Cite this article
Hantke, K. Identification of an iron uptake system specific for coprogen and rhodotorulic acid in Escherichia coli K12. Mol Gen Genet 191, 301–306 (1983). https://doi.org/10.1007/BF00334830
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00334830