Summary
A system for the direct selection of intra- and inter-molecular transposition events has been used to show that intra-molecular transposition of Tn1 generates deletions and inversions and requires the tnpA but not the tnpR gene product, as predicted by current models of transposition. Intra-molecular Tn1 transposition is much less limited by ‘transposition immunity’ than inter-molecular transposition, and occurs at frequencies comparable to those for inter-molecular transposition. The selection system, which uses the bacteriophage λcI-PR region as a target can be used to select, quantify, and characterize any spontaneous or induced mutations.
This is a preview of subscription content, log in via an institution to check access.
References
Arthur A, Sherratt D (1979) Dissection of the transposition process: A transposon-encoded site-specific recombination system. Mol Gen Genet 175:267–274
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heynecker HL, Boyer HW (1979) Construction and characterisation of a new cloning vector. II a multipurpose cloning system. Gene 2:95–113
Chiang SJ, Clowes RC (1980) Intramolecular transposition and inversion in plasmid R6K. J Bacteriol 142:668–682
Datta N, Hedges RW (1972) Trimethoprim resistance conferred by W plasmids in Enterobacteriacae. J Gen Microbiol 72:349–355
Dougan G, Sherratt D (1977) Complementation of transfer deficient ColE1 plasmids. Mol Gen Genet 151:151–160
Finnegan J, Sherratt D (1982) Plasmid ColE1 conjugal mobility: The nature of bom, a region required in cis for transfer. Mol Gen Genet 185:344–351
Heffron F, Bedinger P, Champoux JJ, Falkow S (1977) Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci USA 74:702–606
Kennedy CK (1971) Induction of colicin production by high temperature or inhibition of protein synthesis. J Bacteriol 108:10–19
Kitts PA, Lamond A, Sherratt D (1982) Inter-replicon transposition of Tn1/3 occurs in two sequential genetically separable steps. Nature 295:626–628
Kleckner N (1981) Transposable elements in prokaryotes. Annu Rev Genet 15:341–404
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
Nisen PO, Kopecko DJ, Chou J, Cohen SN (1977) Site specific DNA deletions occurring adjacent to the terminal of a transposable ampicillin resistance element (Tn3). J Mol Biol 117:975–998
Ohtsubo H, Ohmori H, Ohtsubo E (1979) Nucleotide-sequence analysis of Tn3 (Ap): implications for insertion and deletion. Cold Spring Harbor Symp Quant Biol 43:1269–1277
Roberts TM, Swanberg SL, Poteete A, Riedel G, Backman K (1980) A plasmid cloning vehicle allowing a positive selection for inserted fragments. Gene 12:123–127
Robinson MK, Bennett PM, Richmond MH (1977) Inhibition of TnA translocation by TnA. J Bacteriol 128:407–414
Sherratt D, Arthur A, Bishop R, Dyson P, Kitts P, Symington L (1983) Genetic transposition in bacteria. In: Chater KF, Cullis CA, Hopwood DA, Johnston AWB, Woolhouse HW (eds) Genetic re-arrangement. Fifth John Innes Symp. Croom Helm, London and Canberra, pp 59–74
Tacon W, Bhamra S, Sunar B, Sherratt D (1981) Structure and function of plasmid ColK. Plasmid 6:358–359
Weinert TA, Schaus NM, Grindley NDF (1983) Insertion sequences duplication in transpositional recombination. Science 222:755–764
Weinlander L (1979) A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem 98:305
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Bishop, R., Sherratt, D. Transposon Tn1 intra-molecular transposition. Mol Gen Genet 196, 117–122 (1984). https://doi.org/10.1007/BF00334102
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00334102