Summary
A tif-1 umuC36 double mutant of Escherichia coli was constructed. It has been found that the umuC36 mutation prevents both increased spontaneous mutagenesis and enhanced reactivation of UV-irradiated λ, phenomena normally observed in the tif-1 strain grown at 42°C. When the plasmid pKM101 was introduced into tif-1 umuC36, an elevated spontaneous reversion rate of the his-4 mutation observed at 30°C was further increased 6-fold at 42°C. This was accompanied by a 10-fold increase in the ability of tif-1umuC36 containing pKM101 and grown before infection at 42°C to reactivate UV-irradiated λ.
This is a preview of subscription content, log in via an institution to check access.
References
Bagg A, Kenyon CJ, Walker GC (1981) Inducibility of a gene product required for UV and chemical mutagenesis. Proc Natl Acad Sci USA 78:5749–5753
Castelazzi M, George J, Buttin G (1972) Prophage induction and cell division in E. coli. I Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Mol Gen Genet 119:139–152
Fogliano M, Schendel PF (1981) Evidence for the inducibility of the uvrB operon. Nature 289:196–198
George J, Castelazzi M, Buttin G (1975) Prophage induction and cell division in E. coli. III Mutation sfiA and sfiB restore division in tif and lon strains and permit expression of mutator properties of tif. Mol Gen Genet 140:309–332
Goze A, Devoret R (1979) Repair promoted by pKM101 is different from SOS repair. Mutat Res 61:163–179
Gudas LJ, Mount DM (1977) Identification of the recA(tif) gene product of Escherichia coli. Proc Natl Acad Sci USA 74:5280–5284
Kato T, Shinoura Y (1977) Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Mol Gen Genet 156:121–131
Kenyon CJ, Walker GC (1981) Expression of the E. coli uvrA gene is inducible. Nature 289:808–810
Little JW, Edmiston SH, Pacelli LZ, Mount DW (1980) Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci USA 77:3225–3229
Mortelmans KE, Stocker BAD (1979) Segragation of the mutator property of plasmid R46 from its ultraviolet-projecting property. Mol Gen Genet 167:317–327
Shanabruch WG, Walker GC (1980) Localization of the plasmid pKM101 gene(s) involved in recA + lexA+ dependent mutagenesis. Mol Gen Genet 179:289–298
Walker GC (1977) Plasmid pKM101-mediated enhancement of repair and mutagenesis. Dependence on chromosomal genes in Escherichia coli. Mol Gen Genet 152:93–103
Walker GC, Dobson PP (1979) Mutagenesis and repair deficiences of Escherichia coli umuC mutants are suppressed by the plasmid pKM101. Mol Gen Genet 172:17–24
Witkin EM (1976) Ultraviolet mutagenesis and inducuble DNA repair in Escherichia coli. Bateriol Rev 40:869–907
Witkin EM, Wermundsen IE (1980) Targeted and untargeted mutagenesis by various inducers of SOS functions in Escherichia coli. Cold Spring Harbor Symp Quant Biol 43:881–886
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Cieśla, Z. Plasmid pKM101-mediated mutagenesis in Escherichia coli is inducible. Mol Gen Genet 186, 298–300 (1982). https://doi.org/10.1007/BF00331866
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331866