Summary
The morphological changes of human lymphocytes living in vitro under the influence of phytohemagglutinin (PHA) were estimated quantitatively by stereological methods. Mean volume and volume fractions for average human lymphocyte and average blastoid cell from 72-hour culture with PHA were determined. It was found that the lymphocyte volume increases on an average 3.7 times mainly because of cytoplasmic volume increment by 4.3 times. The greatest enlargement concerned endoplasmic reticulum, the nucleolus, lysosomes and mitochondria. In addition a way of correction of the error in experimentally obtained volume fractions, resulting from the exclusive use of sections involving the nucleus is presented.
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References
Bach, F. H., Hirschorn, K.: The in vitro immune response of peripheral blood lymphocytes. Semin. Hemat. 2, 68–89 (1965).
Chapman, J. A., Elves, M. W., Gough, J.: An electron-microscope study of the in vitro transformation of human leukocytes. Transformation of lymphocytes to blastoid cells in the presence of phytohaemagglutinin. J. Cell Sci. 2, 359–370 (1967).
Darżynkiewicz, Z., Dokov, V. K., Pieńkowski, M.: Dry mass of lymphocytes during transformation after stimulation by phytohaemagglutinin. Nature (Lond.) 214, 1265–1266 (1967).
Elias H., Hennig, A., Schwartz, D. E.: Stereology: Applications to biomedical research. Physiol. Rev. 51, 158–200 (1971).
Elves, M. W., Gough, J., Chapman, J. A., Israëls, M. C. G.: Electron microscopy studies of lymphocytes. Transformation under influence of phytohaemagglutinin. Lancet 1964I, 306–308.
Fullman, R. L.: Measurements of particle sizes in opaque bodies. Trans. AIME J. Metals 197, 447–452 (1953).
Inman, D. R., Cooper, E. H.: Electron microscopy of human lymphocytes stimulated by phytohaemagglutinin. J. Cell Biol. 19, 441–445 (1963).
Johnson, F. R., Roberts, K. B.: The growth and division of human small lymphocyte in tissue culture: an electron microscopic study. J. Anat. (Lond.) 98, 303–311 (1964).
Karnovsky, M. J.: Simple methods for “staining with lead” at high pH in electron microscopy. J. biophys. biochem. Cytol. 11, 729–732 (1961).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. Cell Biol. 9, 409–414 (1961).
Michałowski, A.: Phytohaemagglutinin-stimulated division and growth rate of human lymphocytes. Bull. Acad. pol. Sci. (Biol.) 15, 577–581 (1967).
Muñiz, F. J., Houston, E. W., Cruz-Abad, L., Ritzman, S. E., Levin, W. C.: Nuclear volume distribution of phytohemagglutinin-stimulated human lymphocytes. Proc. Soc. exp. Med. 135, 334–339 (1970).
Nowell, P. C.: Phytohaemagglutinin: An initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 20, 462–466 (1960).
Palade, G. E.: A study of fixation for electron microscopy. J. exp. Med. 94, 285–298 (1952).
Sabatini, D. D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Tanaka, Y., Epstein, L. B., Brecher, G., Stohlman, F. Jr.: Transformation of lymphocytes in cultures of human peripheral blood. Blood 22, 614–629 (1963).
Weibel, E. R.: Stereological principles for morphometry in electron microscopic cytology. Int. Rev. Cytol. 19, 235–302 (1969).
Weibel, E. R., Stäubli, W., Gnägi, H. R., Hess, F. A.: Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereologic methods and normal morphometric data for rat liver. J. Cell Biol. 42, 68–91 (1969).
Westring, D. W., Ladinsky, J. L., Feick, P.: The volume distribution of human lymphocytes. Proc. Soc. exp. Biol. (N.Y.) 131, 1077–1083 (1969).
Yoffey, J. M., Winter, G. C. B., Osmond, D. G., Meek, E. S.: Morphological studies in the culture of human leucocytes with phytohaemagglutinin. Brit. J. Haemat. 11, 488–497 (1965).
Zucker-Franklin, D.: The ultrastructure of lymphocytes. Semin. Hemat. 6, 4–27 (1969).
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Konwiński, M., Kozłowski, T. Morphometric study of normal and phytohemagglutinin-stimulated lymphocytes. Z.Zellforsch 129, 500–507 (1972). https://doi.org/10.1007/BF00316745
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DOI: https://doi.org/10.1007/BF00316745