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Quantitative analyses of the constituent membranes of parotid acinar cells and of the changes evident after induced exocytosis

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Summary

A morphometric study has been made at the EM level of Isoproterenol (IPR) induced secretion of rabbit parotid glands in vivo. Emphasis has been placed here on the membrane content of acinar cells and the changes which occur following induced degranulation. In particular it was hoped to establish whether the preservation of zymogen granule membrane as intact electron microscopically visible subunits and the subsequent reutilisation of this membrane is a plausible hypothesis from a quantitative morphological standpoint.

After two hours IPR had caused >95% depletion of granules. About 1343 μm2/cell of granule limiting membrane temporarily fused with the apical plasmalemma during this time and by two hours 1158 μm2/cell of this had been eliminated. Only a small increase in intracellular smooth membrane area was recorded after degranulation and we find no evidence that the zymogen granule membrane is stored indefinitely as smooth membrane fragments either in the region of the Golgi apparatus or elsewhere in the cytoplasm.

IPR caused changes in RER membrane area (+37.7%, 1406 μm2/cell), which is a possible, but we consider implausible relocation site of granule membrane.

The possible mechanism of the removal of ‘excess apical membrane’ and the ultimate fate of the zymogen granule membrane is discussed.

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  1. Department of Human Biology and Anatomy, The University, Sheffield, England

    G. H. Cope & M. A. Williams

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  1. G. H. Cope
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  2. M. A. Williams
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Cope, G.H., Williams, M.A. Quantitative analyses of the constituent membranes of parotid acinar cells and of the changes evident after induced exocytosis. Z.Zellforsch 145, 311–330 (1973). https://doi.org/10.1007/BF00307161

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