Skip to main content
SpringerLink
Log in

A comparison of the immunofluorescent localization of collagen types I, III, and V with the distribution of reticular fibers on the same liver sections of the snow monkey (Macaca fuscata)

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Localizations of collagen types I, III, and V in monkey liver, as determined by the indirect immunofluorescence method, were photographically superimposed on the fibers revealed by silver-staining in the same tissue sections. Immunofluorescence for type I collagen was found to correspond with the brown collagen fibers and with some of the coarse reticular fibers, while that for type III collagen was found to correspond with most, but not all, reticular fibers of the liver as well as with the brown collagen fibers. The distribution of type V collagen coincides not only with the collagen fibers in the stroma of portal triads and around the central veins, but also with the coarse and fine reticular fibers in the liver lobules. By immuno-electron microscopy, reaction products with anti-type III and V collagens antibodies were demonstrated on cross-striated collagen fibrils, about 45 nm in diameter, in the space of Disse. From these observations, it is concluded that: (1) the fine reticular fibers are mainly composed of type III and type V collagens, and (2) the collagen fibers and coarse reticular fibers in the periphery of liver lobules are composed of type I, type III and type V collagens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adachi E, Hayashi T (1985) In vitro formation of fine fibrils with a D-periodic banding pattern from type V collagen. Coll Relat Res 5:225–232

    Google Scholar 

  • Adachi E, Hayashi T (1986) In vitro formation of hybrid fibrils of type V collagen and type I collagen. Connect Tissue Res 14:257–266

    Google Scholar 

  • Adachi E, Hayashi T (1987) Comparison of axial banding patterns in fibrils of type V collagen and type I collagen. Coll Relat Res 7:27–38

    Google Scholar 

  • Adachi E, Hayashi T, Hashimoto PH (1987) Type V collagen in splenic reticular fibers of the macaque monkey. Acta Anat 129:169–175

    Google Scholar 

  • Adachi E, Hayashi T, Hashimoto PH (1989) Immunoelectron microscopical evidence that type V collagen is a fibrillar collagen: importance for an aggregating capability of the preparation for reconstituting banding fibrils. Matrix 9:232–237

    Google Scholar 

  • Aycock RS, Seyer JM (1989) Collagens of normal and cirrhotic human liver. Connect Tissue Res 23:19–31

    Google Scholar 

  • Biempica L, Morecki R, Wu CH, Giambrone MA, Rojkind M (1980) Immunocytochemical localization of type B collagen: a component of basement membrane in human liver. Am J Pathol 98:591–602

    Google Scholar 

  • Birk DE, Fitch JM, Babiarz JP, Linsenmayer TL (1988) Collagen type I and V are present in the same fibril in the avian corneal stroma. J Cell Biol 106:999–1008

    Google Scholar 

  • Bornstein P, Sage H (1980) Structurally distinct collagen types. Ann Rev Biochem 49:957–1003

    Google Scholar 

  • Burt AD, Griffiths MR, Schuppan D, Voss B, Macsween RNS (1990) Ultrastructural localization of extracellular matrix proteins in liver biopsies using ultracryomicrotomy and immunogold labelling. Histopathology 16:53–58

    Google Scholar 

  • Fleischmajer R, Perlish JS, Timpl R (1985) Collagen fibrillogenesis in human skin. In: Fleischmajer R, Olsen BR, Kühn K (eds) Biology, chemistry, and pathology of collagen. Ann NY Acad Sci 460:246–257

  • Fleischmajer R, Perlish JS, Burgeson RE, Shaikh-Bahai F, Timpl R (1990) Type I and type III collagen interactions during fibrillogenesis. In: Fleischmajer R, Olsen BR, Kühn K (eds) Structure, molecular biology, and pathology of collagen. Ann NY Acad Sci 580:161–175

  • Gay S, Martinez-Hernandez A, Rhodes RK, Miller EJ (1981) The collagens exocytoskeleton of smooth muscle cells. Coll Relat Res 1:377–384

    Google Scholar 

  • Geerts A, Geuze HJ, Slot J-W, Voss B, Schuppan D, Schellinck P, Wisse E (1986a) Immunogold localization of procollagen III, fibronectin and heparan sulfate proteoglycan on ultrathin frozen sections of the normal rat liver. Histochemistry 84:355–362

    Google Scholar 

  • Geerts A, Schellinck P, De Zanger RB, Schuppan D, Wisse E (1986b) Fine structrual distribution of procollagen type III in the normal rat liver. Critical investigation of the ‘reticular fiber’ concept. In: Kirn A, Knook DL, Wisse E (eds) Cells of the hepatic sinusoid, vol 1. Kupffer Cell Foundation, Rìjswìjk, The Netherlands, pp 221–226

    Google Scholar 

  • Gomori G (1937) Silver impregnation of reticulum in paraffin sections. Am J Pathol 13:993–1002

    Google Scholar 

  • Grimaud J-A, Druguet M, Peyrol S, Chevalier O, Herbage D, Badrawy NE (1980) Collagen immunotyping in human liver: Light and electron microscope study. J Histochem Cytochem 28:1145–1156

    Google Scholar 

  • Hahn E, Wick G, Pencev D, Timpl R (1980) Distribution of basement membrane protein in normal and fibrotic human liver: collagen type IV, laminin, and fibronectin. Gut 21:63–71

    Google Scholar 

  • Howard PS, Macarak EJ (1989) Localization of collagen types in regional segments of the fetal bovine aorta. Lab Invest 61:548–555

    Google Scholar 

  • Ishii T (1968) Über den lichtmikroskopischen Feinbau der Retikulumfasern im Lymphknoten (ein Beitrag zur Frage der Natur der Retikulumfasern). Anat Anz [Suppl] 121:487–494

    Google Scholar 

  • Junqueira LCU, Montes GS (1983) Biology of collagen-proteoglycan interaction. Arch Histol Jpn 46:589–629

    Google Scholar 

  • Keene DR, Sakai LY, Bächinger HP, Burgeson RE (1987) Type III collagen can be present on banded collagen fibrils regardless of fibril diameter. J Cell Biol 105:2393–2402

    Google Scholar 

  • Konomi H, Sano J, Nagai Y (1981) Immunohistochemical localization of type I, III and type IV collagens in the liver. Acta Pathol Jpn 31:973–978

    Google Scholar 

  • Konomi H, Hayashi T, Nakayasu K, Arima M (1984) Localization of type V collagen and type IV collagen in human cornea, lung and skin. Immunohistochemical evidence by anti-collagen antibodies characterized by immunoelectroblotting. Am J Pathol 116:417–426

    Google Scholar 

  • Lapiere CM, Nusgens B, Pierard GE (1977) Interactions between collagen type I and type III in conditioning bundles organization. Connect Tissue Res 5:21–29

    Google Scholar 

  • Linsenmayer TF, Fitch JM, Schmid TM, Zak NB, Gibney E, Sanderson RD, Mayne R (1983) Monoclonal antibodies against chicken type V collagen: production, specificity, and use for immunocytochemical localization in embryonic cornea and other organs. J Cell Biol 96:124–132

    Google Scholar 

  • Linsenmayer TF, Fitch JM, Birk DE (1990) Heterotypic collagen fibrils and stabilizing collagens; Controlling elements in corneal morphogenesis? In: Fleischmajer R, Olsen BR, Kühn K (eds) Structure, molecular biology, and pathology of collagen. Ann NY Acad Sci 580:143–160

  • Madri JA, Furthmayr H (1979) Isolation and tissue localization of type AB2 collagen from normal lung parenchyma. Am J Pathol 94:323–332

    Google Scholar 

  • Martinez-Hernandez A (1984) The hepatic extracellular matrix I. Electron immunohistochemical studies in normal rat liver. Lab Invest 51:57–74

    Google Scholar 

  • Martinez-Hernandez A, Gay S, Miller EJ (1982) Ultrastructural localization of type V collagen in rat kidney. J Cell Biol 92:343–349

    Google Scholar 

  • McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative, a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083

    Google Scholar 

  • Mendler M, Eich-Bender SG, Vaughan L, Winterhalter KH, Bruckner P (1989) Cartilage contains mixed fibrils of collagen types II, IX, and XI. J Cell Biol 108: 191–197

    Google Scholar 

  • Miller EJ, Gay S (1987) The collagens: an overview and update. In: Cunningham LW (ed) Methods enzymol, vol 144. Academic Press, New York London, pp 3–41

    Google Scholar 

  • Miller EJ, Rhodes RK (1982) Preparation and characterization of the different types of collagen. In: Cunningham LW, Fredericksen DW (eds) Methods enzymol, vol 82. Academic Press, New York London, pp 33–64

    Google Scholar 

  • Modesti A, Kalebic T, Scarpa S, Togo S, Grotendorst G, Liotta LA, Triche TJ (1984) Type V collagen in human amnion is a 12 nm fibrillar component of the pericellular interstitium. Eur J Cell Biol 35:246–255

    Google Scholar 

  • Nakayasu K, Konomi H, Hayashi T, Arima M, Kanai A, Nakajima A (1986) Distribution of type I, II, III, IV and V collagen in normal and keratoconus corneas. Ophtalmic Res 18:1–10

    Google Scholar 

  • Ohtani O (1988) Three-dimensional organization of the collagen fibrillar framework of the human and rat livers. Arch Histol Cytol 51:473–488

    Google Scholar 

  • Rojkind M, Ponce-Noyola P (1982) The extracellular matrix of the liver. Collagen Relat Res 2:151–175

    Google Scholar 

  • Roll FJ, Madri JA, Albert J, Furthmayr H (1980) Codistribution of type IV and AB2 in basement membranes and mesangium of the kidney. J Cell Biol 85:597–616

    Google Scholar 

  • Sano J, Sato S, Isizaki M, Yajima G, Konomi H, Fujiwara S, Nagai Y (1981) Types I, III and IV (basement membrane) collagens in the bovine liver parenchyma: electron microscopic localization by the peroxidase-labeled antibody method. Biomed Res 2:546–551

    Google Scholar 

  • Sato S, Leo MA, Lieber CS (1986) Ultrastructural localization of type III procollagen in baboon liver. Am J Pathol 122:212–217

    Google Scholar 

  • Schuppan D, Becker J, Boehm H, Hahn EG (1986) Immunofluorescent localization of type-V collagen as a fibrillar component of the interstitial connective tissue of human oral mucosa, artery and liver. Cell Tissue Res 243:535–543

    Google Scholar 

  • Smith LT, Holbrook KA, Madri JA (1986) Collagen types I, III, and V in human embryonic and fetal skin. Am J Anat 175:507–521

    Google Scholar 

  • Snodgrass MJ (1977) Ultrastructural distinction between reticular and collagenous fibers with an ammoniacal silver stain. Anat Rec 187:191–206

    Google Scholar 

  • Voss B, Rauterberg J, Allam S, Pott G (1980) Distribution of collagen type I and type III and of two collagenous components of basement membranes in the human liver. Path Res Pract 170:50–60

    Google Scholar 

  • Yamamoto M, Sumiyoshi H, Nakagami K, Tahara E (1984) Distribution of collagen type I, III and V in fibrotic and neoplastic human liver. Acta Pathol Jpn 34:77–86

    Google Scholar 

Download references

Author information

Author notes

  1. E. Adachi

    Present address: Department of Biochemistry and Molecular Biology, Jefferson, Institute of Molecular Medicine, 479 Jefferson Alumni Hall, 1020 Locust Street, 19107, Philadelphia, PA, USA

Authors and Affiliations

  1. Department of Anatomy, Osaka University Medical School, Osaka, Japan

    E. Adachi & P. H. Hashimoto

  2. Department of Chemistry, College of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153, Tokyo, Japan

    T. Hayashi

Authors
  1. E. Adachi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. H. Hashimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adachi, E., Hayashi, T. & Hashimoto, P.H. A comparison of the immunofluorescent localization of collagen types I, III, and V with the distribution of reticular fibers on the same liver sections of the snow monkey (Macaca fuscata). Cell Tissue Res 264, 1–8 (1991). https://doi.org/10.1007/BF00305716

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00305716

Key words

Search

Navigation