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Influence of temperature on the detectibility and chromosomal distribution of specific DNA sequences by in situ hybridisation

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Abstract

Hybridising certain AT-rich satellite complementary RNAs (cRNAs) to their homologous chromosomal DNA sequences at different temperatures of incubation results in a different dispersion of autoradiographic label throughout the karyotypes. The temperature at which most label, or eRNA-DNA hybrid formation, exists corresponds to the optimal rate temperature for the hybridisation of these same satellite cRNA-DNA hybrids as determined by RNA excess filter hybridisation. It is likely that the in situ hybridisation results can therefore be explained by the fact that there is a similar temperature-dependence on the rate of hybrid formation for both in situ and RNA excess hybridisation. This should have important implications for the designing of in situ hybridisation experiments in general.

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Authors and Affiliations

  1. Institute of Animal Genetics, University of Edinburgh, West Mains Road, EH9 3JN, Edinburgh, Scotland

    M. H. Moar, I. F. Purdom & K. W. Jones

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  1. M. H. Moar
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  2. I. F. Purdom
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  3. K. W. Jones
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Moar, M.H., Purdom, I.F. & Jones, K.W. Influence of temperature on the detectibility and chromosomal distribution of specific DNA sequences by in situ hybridisation. Chromosoma 53, 345–359 (1975). https://doi.org/10.1007/BF00294082

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  • DOI: https://doi.org/10.1007/BF00294082

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