Abstract
The influence of chloral hydrate on endosperm cells of Haemanthus katherinae was studied. Four types of mitotic disturbances were described: a) Normal bipolar mitosis with one or few chromosomes lying out of the metaphase plate. Bipolar mitoses are sometimes arrested in anaphase and followed by the formation of a restitution nucleus. b) Tri — or multipolar anaphases. c) “Diffuse anaphase” movements i.e., active anaphase movements without distinct poles followed by the formation of multipolar phragmoplasts and cell walls. d) C-mitosis resembling the effect of colchicine. Some aspects of the described phenomena were discussed.
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Bajer, A.: Cine-micrographic studies on chromosome movements in-irradiated cells. Chromosoma (Berl.) 9, 319–331 (1958); - A note on the behaviour of spindle fibres at mitosis. Chromosoma (Berl.) 12, 64–71 (1961); - Note on ultrastructure and some transport properties within the mitotic spindle. J. Cel. Biol. (in press) (1967).
—, and R. D. Allen: Structure and Organization of the living mitotic spindle of Haemanthus katharinae. Science 151, 572–574 (1966).
—, and J. Molé-Bajer: Cine-micrographic studies on mitosis in endosperm. II. Chromosoma (Berl.) 7, 558–607 (1956); - Cine analysis of some aspects of mitosis in endosperm. In: Cinematography in cell biology. New York: Academic Press 1963.
Biesele, J. J.: Mitotic poisons and the cancer problem, p. 214. Amsterdam: Elsevier Publ. Co. 1958.
Cummins, J. E., J. C. Blomquist, and H. P. Rusch: Anaphase delay after inhibition of protein synthesis between late prophase and prometaphase. Science 154, 1343–1344 (1966).
Dietz, R.: Die Spermatocytenteilungen der Tipuliden. II. Chromosoma (Berl.) 8, 183–211 (1956);- Centrosomenfreie Spindelpole in Tipulidenspermatocyten. Z. Naturforsch. 14d, 749–752 (1959).
Dustin jr., P., and R. Parmentier: Donnés nouvelles sur les mitoses à chromosomes polaires. Atti Simp. antimitot. 23–40 (1955).
Gavaudan, P.: Étude quantitative de l'action mito-inhibitrice des substances aromatiques: Définition et terminologie des effects cytologiques comme test. C. R. Soc. Biol. (Paris) 137, 281–283 (1943); - Pharmacodynamie de l'inhibition de la caryokinese, p. 340. Paris: Librairie le François 1947.
Inoué, S.: The effect of colchicine on the microscopic and submicroscopic structure of the mitotic spindle. Exp. Cell Res., Suppl. 2, 305–311 (1951).
—, and A. Bajer: Birefringence in endosperm mitosis. Chromosoma (Berl.) 12, 48–63 (1961).
Iztusu, K., and J. J. Biesele: Effects on HeLa cell division of physiologic deoxyribonucleosides and deoxyribonucleotides. Cancer Res. 26, 910–928 (1966).
Kihlman, B. A.: Actions of chemicals on dividing cells. Englewood Cliffs, New Jersey: Prentice-Hall, Inc. 1966.
Levan, A.: The effect of colchicine on root mitosis in Allium. Hereditas (Lond.) 24, 471–486 (1938); - The effect of acenaphten and colchicine on mitosis of Allium and Colchicum. Hereditas (Lund) 26, 262–276 (1940).
—, and G. Östergren. The mechanism of c-mitotic action. Observations on the naphtalene series. Hereditas (Lund) 29, 381–443 (1943).
Mazia, D.: The organization of the mitotic apparatus. Symp. Soc. exp. Biol. IX.: Fibrous proteins and their biological significance. 335–357 (1955); - Some problems in the chemistry of mitosis. In: The chemical basis of heredity, p. 169 to 185. Baltimore, Maryland: Johns Hopkins Press 1957.
—, and K. Dan: The isolation and biochemical characterization of the mitotic apparatus of dividing cells. Proc. nat. Acad. Sci. (Wash.) 38, 826–838 (1952).
Molé-Bajer, J.: Cinematographic analysis of c-mitosis in endosperm. Chromosoma (Berl.) 9, 332–358 (1958); - Telophase segregation of chromosomes and amitosis. J. Cell Biol. 25, 79–93 (1965).
—, and A. Bajer: Mitosis in endosperm. Techniques of studies in vitro. Cellule 43, 399–407 (1963).
Něměc, B.: Über die Einwirkung des Chloralhydrates auf Kern und Zellteilung. Jb. wiss. Bot. 39, 645–730 (1903).
Nicklas, B.: An experimental and descriptive study of chromosome elimination in Miastor spec. Chromosoma (Berl.) 10, 301–336 (1959); - The chromosome cycle of a primitive cecidiomyid — Mycophila speyeri. Chromosoma (Berl.) 11, 402–418 (1960); - Recurrent pole-to-pole movements of the sex chromosome during prometaphase I in Melanopus differentialis spermatocytes. Chromosoma (Berl.) 12, 97–115 (1961).
Östergren, G.: Colchicine mitosis, chromosome contraction, narcosis and protein chain folding. Hereditas (Lund) 30, 429–467 (1944); - Cytological standards for the quantitative estimation of spindle disturbances. Hereditas (Lund) 36, 371–382 (1950).
—, and A. Bajer: La mitose dans les cellules traitées par le mèthanol. Coll. Int. Centre Nat. Rech. Sci. 88, 199–207 (1960).
Regemorter, D. van: Les troubles cinétiques dans les racines chloralisées. Cellule 37, 43–73 (1926).
Ris, H.: The anaphase movement of chromosomes in the spermatocytes of the grasshopper. Biol. Bull. 96, 90–106 (1949).
Sakamura, T.: Über die Beeinflussung der Zell- und Kernteilung durch Chloralisierung mit besonderer Berücksichtigung des Verhaltens der Chromosomen. Bot. Mag. Tokyo 30, 375–399 (1916).
Sentein, P.: L'action de la vincaleucoblastine sur la mitose chez Triturus helveticus Raz. Chromosoma (Berl.) 15, 416–456 (1964).
Schrader, F.: Mitosis, 2nd ed. p. 170. New York: Columbia Univ. Press 1953.
Siebs, W.: Mitoseablaufstörungen. I. Polchromosomen und dreigruppen Metaphasen. Z. Zellforsch. 51, 497–534 (1960).
Strasburger, E.: Über die Individualität der Chromosomen und die Propfhybridenfrage. Jb. wiss. Bot. 44, 482–555 (1907).
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Molé-Bajer, J. Chromosome movements in chloral hydrate treated endosperm cells in vitro . Chromosoma 22, 465–480 (1967). https://doi.org/10.1007/BF00286548
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DOI: https://doi.org/10.1007/BF00286548