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Binding of quinacrine to the human Y chromosome

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Abstract

Human chromosome spreads were stained with 3H-quinacrine and their fluorescence observed. The exact location of specific spreads on each slide was noted and photographs taken. Autoradiographs were then prepared so that the quinacrine fluorescence of any specific chromosome could be compared directly with the distribution of grains over the same chromosome on the autoradiograph. The Y chromosome fluoresced much more intensely than any of the other chromosomes, but there were no more grains over the Y chromosome than over the other chromosomes. Therefore the enhanced fluorescence of the human Y chromosome is not due to an increased binding of quinacrine.

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Authors and Affiliations

  1. MRC Mammalian Genome Unit, Department of Zoology, University of Edinburgh, West Mains Road, EH9 3 JT, Edinburgh, Scotland

    J. M. R. Hatfield, K. W. C. Peden & R. M. West

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  1. J. M. R. Hatfield
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  2. K. W. C. Peden
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  3. R. M. West
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Hatfield, J.M.R., Peden, K.W.C. & West, R.M. Binding of quinacrine to the human Y chromosome. Chromosoma 52, 67–71 (1975). https://doi.org/10.1007/BF00285790

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  • DOI: https://doi.org/10.1007/BF00285790

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