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Methods of increasing the visibility of fragile X chromosomes

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Summary

A comparison was made between different microscopic techniques in order to determine which is the best method for recognizing the fragile sites on X chromosomes. Orceinstained mitoses observed in phase contrast showed the highest percentage of fragile X chromosomes. Similar results were obtained by reflection contrast microscopy of mitoses stained briefly with Giemsa solution. Nomarski interference contrast was less suitable and brightfield microscopy was the least suitable technique of those tested.

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References

  • Patzelt WJ (1977) Reflexionskontrast — eine neue lichtmikroskopische Technik. Mikrokosmos: 78–81

  • Schwarzacher HG (1974) Preparation of metaphase chromosomes. In: Schwarzacher HG, Wolf U (eds) Methods of human cytogenetics. Springer, Berlin, pp 71–81

    Google Scholar 

  • Sutherland GR (1979) Heritable fragile sites on human chromosomes. I. Factors affecting expression in lymphocyte culture. Am J Hum Genet 31:125–135

    Google Scholar 

  • Turner G, Opitz JM (1980) X-linked mental retardation. Am J Med Genet 7:407–415

    Google Scholar 

  • Turner G, Daniel A, Frost M (1980) X-linked mental retardation, macro-orchidism and the Xq27 fragile site. J Pediatr 96:837–841

    Google Scholar 

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Authors and Affiliations

  1. Division of Human Biology and Human Genetics, University of Kaiserslautern, Pfaffenbergstraße, D-6750, Kaiserslautern, Federal Republic of Germany

    Heinrich Zankl & Gertrud Eberle

Authors
  1. Heinrich Zankl
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  2. Gertrud Eberle
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Zankl, H., Eberle, G. Methods of increasing the visibility of fragile X chromosomes. Hum Genet 60, 80–81 (1982). https://doi.org/10.1007/BF00281271

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  • DOI: https://doi.org/10.1007/BF00281271

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