Summary
The mutation recL152 leads to a reduction of excision repair as measured by an increase in the time required to close uvrA uvrB dependent incision breaks, and by a reduction of host cell reactivation ability. Postreplication repair is also delayed when measured in a uvrB5 recL152 double mutant. Such a determination could not be made using the recL152 single mutant because the excision defect led to an accumulation of breaks in the unlabeled high molecular weight DNA to which the labeled DNA synthesized after irradiation must attach in order to achieve normal high molecular weight. Further, the recL gene product seems to be required to rejoin breaks in parental strand DNA which are generated during postreplication repair, since such gaps accumulate in a recL152 uvrB5 double mutant but not in a recL + uvrB5 single mutant. We have noticed a striking phenotypic similarity between recL152 and polA1 and suggest that recL152 is required for full in vivo activity of DNA polymerase I.
Similar content being viewed by others
References
Abelson, J., Thomas, C.A.: The anatomy of the T5 bacteriophage DNA molecule. J. Mol. Biol. 18, 262–291 (1966)
Bachmann, B.J.: Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36, 525–557 (1972)
Bachmann, B.J., Low, K.B., Tylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bacteriol. Rev. 40, 116–167 (1976)
Boyce, R.P., Setlow, R.B.: A simple method of incresing the incorporation of thymidine into the deoxyribonucleic acid of Escherichia coli. Biochim. Biophys. Acta 61, 618–620 (1962)
Boyce, R.P., Howard-Flanders, P.: Release of ultraviolet lightinduced thymine dimers from DNA in E. coli K-12. Proc. Nat. Acad. Sci., U.S.A. 51, 293–300 (1964)
Brendel, M., Haynes, R.H.: Interactions among genes controlling sensitivity to radiation and alkylation in yeast. Molec. gen. Genet. 125, 197–216 (1973)
Bridges, B.A.: Recent advances in basic mutation research. Address to the 6th annual meeting of the European Environmental Mutagen Society at Gernrode, Oct. 1976 (1977)
Clark, A.J., Margulies, A.D.: Isolation and characterization of recombination deficient mutants of Escherichia coli K-12. Proc. Nat. Acad. Sci., U.S.A. 52, 451–459 (1965)
Clark, A.J.: Recombination deficient mutants of E. coli and other bacteria. Ann. Rev. Genetics 7, 67–86 (1973)
Demerec, M., Adelberg, E.A., Clark, A.J., Hartman, P.E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–67 (1966)
Freifelder, D.: Molecular weights of coliphages and coliphage DNA IV. Molecular weights from bacteriophages T4, T5 and T7 and the general problem of determination of M. J. Mol. Biol. 54, 567–577 (1970)
Ganesan, A.K., Smith, K.C.: Dark recovery processes in Escherichia coli irradiated with ultraviolet light III. Effect of rec mutations on recovery of excision-deficient mutants of Escherichia coli K-12. J. Bacteriol. 102, 404–410 (1970)
Gillin, F.D., Nossal, N.G.: Control of mutation frequency by bacteriophage T4 DNA polymerase I. The CB120 antimutator DNA polymerase is defective in strand displacement. J. Biol. Chem. 17, 5219–5224 (1976)
Goodman, M.F., Gore, W.C., Muzyczka, N., Bessman, M.J.: Studies on the biochemical basis of spontaneous mutation III. Rate model for DNA polymerase-effected nucleotide misincorporation. J. Mol. Biol. 88, 423–435 (1975)
Horii, Z-I., Clark, A.J.: Genetic analysis of the RecF pathway to genetic recombination in Escherichia coli: isolation and characterization of mutants. J. Mol. Biol. 80, 327–344 (1973)
Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175–200 (1968)
Kanner, L., Hanawalt, P.: Repair deficiency in a bacterial mutant defective in DNA polymerase. Biochem. Biophys. Res. Comm. 39, 149–155 (1970)
Kato, T.: Excision repair characteristics of recB -res- and uvrC - strains of Escherichia coli. J. Bacteriol. 112, 1237–1246 (1972)
Klein, A., Niebch, U.: Host-cell reactivation in strains of E. coli lacking DNA polymerase activity in vitro. Nature New Biology 229, 82–84 (1971)
Latarjet, R., Morenne, R., Berger, R.: Un appareil simple pour le dosage des rayonnements ultraviolets emis par les lampes germicides. Annales de l'Inst. Past. 85, 174–184 (1953)
Ley, R.D.: Ultraviolet-light-induced incorporation of bromodeoxyuridine into parental DNA of an excision deficient cell of Escherichia coli. In Molecular mechanisms for repair of DNA (Eds. Hanawalt, P.C. and Setlow, R.B.), pp 313–316. New York, New York: Plenum Press 1975
Mattern, I.E., Houtman, P.C.: Properties of uvrE mutants of Escherichia coli K-12 II. Construction and properties of pol and rec derivatives. Mutation Res. 25, 281–287 (1974)
McKeown, M., Kahn, M., Hanawalt, P.: Thymidine uptake and ultilization in Escherichia coli: a new gene controlling nucleoside transport. J. Bacteriol. 126, 814–822 (1976)
Monk, M., Peacey, M., Gross, J.D.: Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells. J. Mol. Biol. 58, 628–630 (1971)
Radman, M.: Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis. In Molecular and environmental aspects of mutagenesis (Eds. Prakash, L., Sherman, F., Miller, M., Lawrence, C.W., and H. Taber) Chapter 8, Springfield, Ill.: C.C. Thomas 1974
Rupp, W.D., Howard-Flanders, P.: Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J. Mol. Biol. 31, 291–304 (1968a)
Rupp, W.D., Howard-Flanders, P.: Sedimentation and equilibrium density gradient methods in the study of post-replication repair. In Methods in enzymology (Eds. Grossman, L., and Moldave, K.) pp. 237–244. New York: Academic Press 1958b
Rupp, W.D., Wilde III, C.E., Reno, D.L., Howard-Flanders, P.: Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J. Mol. Biol. 61, 25–44 (1971)
Sedgwick, S.G.: Genetic and kinetic evidence for different types of postreplication repair in Escherichia coli B. J. Bacteriol. 123 154–161 (1975a)
Sedgwick, S.G.: Inducible error-prone repair in Escherichia coli Proc. Nat. Acad. Sci., U.S.A. 72, 2753–2757 (1975b)
Seliakova, M., Brozmanova, J., Slezarikova, V., Masek, F., Fandlova, E.: Function of the UVR marker in dark repair of DNA molecules. Neoplasma 22, 361–384 (1975)
Setlow, R.B., Carrier, W.L.: The disappearance of thymine dimers from DNA: An error correcting mechanism. Proc. Nat. Acad. Sci., U.S.A. 51, 226–231 (1964)
Sinzinis, B.I., Smirmov, G.B., Saenko, A.S.: Repair deficiency in Escherichia coli UV-sensitive mutator strain uvr502. Biochem. Biophys. Res. Comm. 53, 309–316 (1973)
Smirnov, G.B., Filkova, E.V., Skavronskaya, A.G.: Ultraviolet sensitivity, spontaneous mutability and DNA degradation in Escherichia coli strains carrying mutations in uvr and rec genes. J. Gen. Microbiol. 76, 407–416 (1973)
Smirnov, G.B., Filkova, E.V., Skavronskaya, A.G., Saenko, A.D., Sinzinis, B.I.: Loss and restoration of viability of E. coli due to combinations of mutations affecting DNA polymerase I and repair activities. Molec. gen. Genet. 121, 139–150 (1973)
Smith, K.C., Meun, D.H.C.: Repair of radiation induced damage in Escherichia coli I. Effect of rec mutations on postreplication repair of damage due to ultraviolet radiation. J. Mol. Biol. 51, 459–472 (1970)
Tait, R.C., Harris, A.L., Smith, D.W.: DNA repair in Escherichia coli mutants deficient in DNA polymerases I, II and III. Proc. Nat. Acad. Sci., U.S.A. 71, 675–679 (1974)
Van Sluis, C.A., Mattern, I.E., Paterson, M.C.: Properties of uvrE mutants of Escherichia coli K12 I. Effects of UV irradiation on DNA metabolism. Mutn. Res. 25, 273–279 (1974)
Willets, N.S., Clark, A.J., Low, K.B.: Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J. Bacteriol. 97, 244–249 (1969)
Witkin, E.M.: Mutation-proof and mutation-prone modes of survival in derivatives of Escherichia coli B differing in sensitivity to ultraviolet light. Brookhaven Symp. Biol. 20, 17–55 (1967)
Youngs, D.A., Smith, K.C.: The involvenent of polynucleotide ligase in the repair of UV-induced DNA damage in Escherichia coli K12 cells. Molec. gen. Genet. in press (1977)
Author information
Authors and Affiliations
Additional information
Communicated by E. Witkin
Rights and permissions
About this article
Cite this article
Rothman, R.H., Clark, A.J. Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12. Molec. Gen. Genet. 155, 267–277 (1977). https://doi.org/10.1007/BF00272805
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272805