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Inhibition of deoxyribonuclease activity associated with soybean chloroplasts

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Abstract

The effects of spermidine, pH, ethylene diamine tetracetic acid (EDTA), and adenosine triphosphate (ATP) on deoxyribonuclease (DNase) activity associated with the chloroplasts of soybean (Glycine max (L.) Merr.) were investigated. Chloroplast DNase activity was found to be partially inhibited by either 10 mM spermidine, 20 mM EDTA, or 20 mM ATP. DNase activity was also partially inhibited at non-neutral pH's. Nearly complete inhibition was achieved with use of 30 mM EDTA, pH 10, or a combination of 10 mM spermidine and 10 mM EDTA.

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Abbreviations

EDTA:

Ethylene diamine tetraacetic acid

ATP:

Adenosine triphosphate

DNase:

Deoxyribonuclease

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Authors and Affiliations

  1. Department of Genetics, Iowa State University, 50011, Ames, IA, USA

    R. C. Shoemaker & A. G. Atherly

  2. Research Geneticist, ARS-USDA, Department of Agronomy and Genetics, Iowa State University, 50011, Ames, IA, USA

    R. G. Palmer

Authors
  1. R. C. Shoemaker
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  2. A. G. Atherly
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  3. R. G. Palmer
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Shoemaker, R.C., Atherly, A.G. & Palmer, R.G. Inhibition of deoxyribonuclease activity associated with soybean chloroplasts. Plant Cell Reports 2, 98–100 (1983). https://doi.org/10.1007/BF00270176

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  • DOI: https://doi.org/10.1007/BF00270176

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