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Location on the chromosome of Escherichia coli of genes governing purine metabolism

Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt)

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Summary

Genes coding for enzymes functioning in purine salvage pathways have been located on the chromosome of Escherichia coli. The gene add encoding adenosine deaminase was located by transduction at 31 min, the gene order was established to be man-uidA-add-aroD. A deletion covering man-uidA-add was obtained. The gene gsk encoding guanosine kinase was cotransducible with purE and shown to be located at 13 min. The gene hpt encoding hypoxanthine phosphoribosyltransferase was contransducible with tonA indicating a location at 3 min. The location of the gene gpt encoding guanine (xanthine) phosphoribosyltransferase in the proA-proB region was confirmed.

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Abbreviations

sAMP:

succinyl-AMP

IMP:

inosine monophosphate

XMP:

xanthosine monophosphate

Ado:

adenosine

Guo:

guanosine

Ino:

inosine

Ade:

adenine

Hyp:

hypoxanthine

Gua:

guanine

Xan:

xanthine

dAdo:

deoxyadenosine

AI-CAR:

5′ phosphoribosyl-5 amino-4 imidazole carboxamide

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Authors and Affiliations

  1. University Institute of Biological Chemistry B, Copenhagen, Denmark

    Bjarne Jochimsen, Per Nygaard & Trygvi Vestergaard

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  1. Bjarne Jochimsen
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  2. Per Nygaard
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  3. Trygvi Vestergaard
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Communicated by E. Bautz

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Jochimsen, B., Nygaard, P. & Vestergaard, T. Location on the chromosome of Escherichia coli of genes governing purine metabolism. Molec. Gen. Genet. 143, 85–91 (1975). https://doi.org/10.1007/BF00269424

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  • DOI: https://doi.org/10.1007/BF00269424

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