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A cleavage map of the TOL plasmid of Pseudomonas putida mt-2

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Summary

A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants.

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References

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Authors and Affiliations

  1. Department of Molecular Biology, University of Edinburgh, Kings Buildings, EH9 3JR, Edinburgh, Scotland

    Robert Downing & Paul Broda

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  1. Robert Downing
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  2. Paul Broda
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Communicated by E. Bautz

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Downing, R., Broda, P. A cleavage map of the TOL plasmid of Pseudomonas putida mt-2. Molec. Gen. Genet. 177, 189–191 (1979). https://doi.org/10.1007/BF00267270

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  • DOI: https://doi.org/10.1007/BF00267270

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