Summary
To elucidate the effect of adsorption of cellulases during hydrolysis of crystalline cellulose, the relationship between the rate of hydrolysis and the adsorption of crude cellulases onto crystalline cellulose was investigated under various experimental conditions. Several phases of adsorption have been proposed to explain the process of cellulose hydrolysis by these enzymes. The process of hydrolysis calculated on the basis of these phases fitted well with that obtained experimentally.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beltrame PL, Carniti P, Focher B, Marzetti A, Cattaneo M (1982) Cotton cellulose: Enzyme adsorption and enzymatic hydrolysis. J Appl Polym Sci 27:3493–3502
Castanon M, Wilke C (1980) Adsorption and recovery of cellulases during hydrolysis of newspaper. Biotechnol Bioeng 22:1037–1053
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Howell JA, Stuck JD (1975) Kinetics of Solka Floc cellulose hydrolysis by Trichoderma viride cellulase. Biotechnol Bioeng 17:873–893
Lee SB, Shin HS, Ryu DDY, Mandels M (1982) Adsorption of cellulase on cellulose: Effect of physicochemical properties of cellulose on adsorption and rate of hydrolysis. Biotechnol Bioeng 24:2137–2153
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mandels M, Kostick J, Parizek R (1971) The use of adsorbed cellulase in the continuous conversion of cellulose to glucose. J Polym Sci (Part C) 36:445–459
Matsuno R, Taniguchi M, Tanaka M, Kamikubo T (1984) A model for hydrolysis of microcrystalline cellulose by cellulase. Ann New York Acad Sci 434:158–160
Moloney A, Coughlan MP (1983) Sorption of Talaromyces emersonii cellulase on cellulosic substrates. Biotechnol Bioeng 25:271–280
Mukataka S, Tada M, Takahashi J (1983) Effects of agitation on enzymatic hydrolysis of cellulose in a stirred-tank reactor. J Ferment Technol 61:615–621
Stone JE, Scallan AM, Donefer E, Ahlgren E (1969) Digestibility as a simple function of a molecule of similar size to a cellulase enzyme. Adv Chem Ser 95:219–241
Tanaka M, Takenawa S, Matsuno R, Kamikubo T (1977) Purification and properties of cellulases from Pellicularia filamentosa. J Ferment Technol 55:137–142
Tanaka M, Takenawa S, Matsuno R, Kamikubo T (1978) Some factors affecting cellulose degradation with Pellicularia filamentosa cellulases. J Ferment Technol 56:108–113
Tsao GT, Ladisch M, Ladisch C, Hsu TA, Dale B, Chou T (1978) Fermentation substrates from cellulosic materials: Production of fermentable sugars from cellulosic materials. Ann Rep Ferment Proc 2:1–21
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tanaka, M., Nakamura, H., Taniguchi, M. et al. Elucidation of adsorption processes of cellulases during hydrolysis of crystalline cellulose. Appl Microbiol Biotechnol 23, 263–268 (1986). https://doi.org/10.1007/BF00261926
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00261926