Cell Biology and Toxicology

, Volume 8, Issue 2, pp 157–170

Comparison of in vivo acute lethal potency and in vitro cytotoxicity of 48 chemicals

  • R. Shrivastava
  • C. Delomenie
  • A. Chevalier
  • G. John
  • B. Ekwall
  • E. Walum
  • R. Massingham
Article

DOI: 10.1007/BF00260565

Cite this article as:
Shrivastava, R., Delomenie, C., Chevalier, A. et al. Cell Biol Toxicol (1992) 8: 157. doi:10.1007/BF00260565

Abstract

The cytotoxicity of 48 compounds included in the MEIC (Multicenter Evaluation of In Vitro Cytotoxicity) list was determined in cultures of rat hepatocytes, McCoy, and MDBK cells. The average minimum concentration of each compound inducing cytotoxicity was measured in each cell type. The cytotoxicity values were then compared with published oral LDS p values for rats and mice. The logarithmic transformation of in vivo toxic doses and the corresponding in vitro cytotoxic concentrations showed a statistically significant correlation between the in vitro and in vivo values. The results show that an accurate in vivo LDS p dose could be predicted from in vitro data for at least 75% of the selected compounds. It is hoped that this finding will not only stimulate others to pursue in vitro technique but will eventually lead to elimination of the in vivo LD50 test.

Abbreviations

CT50

50% cytotoxic concentration

CT100

100% cytotoxic concentration

DMSO

dimethyl sulfoxide

LD50

50% lethal dose

LDH

Lactate dehydrogenase

MEIC

Multicenter Evaluation of In Vitro Cytotoxicity

Copyright information

© Princeton Scientific Publishing Co., Inc 1991

Authors and Affiliations

  • R. Shrivastava
    • 1
  • C. Delomenie
    • 1
  • A. Chevalier
    • 1
  • G. John
    • 1
  • B. Ekwall
    • 2
  • E. Walum
    • 3
  • R. Massingham
    • 1
  1. 1.RL-CERMRiomFrance
  2. 2.Department of ToxicologyUniversity of UppsalaSweden
  3. 3.Department of Neurochemistry and NeurotoxicologyUniversity of StockholmSweden
  4. 4.RL-CERMRiom CedexFrance