Cell Biology and Toxicology

, Volume 8, Issue 2, pp 157-170

First online:

Comparison of in vivo acute lethal potency and in vitro cytotoxicity of 48 chemicals

  • R. ShrivastavaAffiliated withRL-CERM
  • , C. DelomenieAffiliated withRL-CERM
  • , A. ChevalierAffiliated withRL-CERM
  • , G. JohnAffiliated withRL-CERM
  • , B. EkwallAffiliated withDepartment of Toxicology, University of Uppsala
  • , E. WalumAffiliated withDepartment of Neurochemistry and Neurotoxicology, University of Stockholm
  • , R. MassinghamAffiliated withRL-CERM

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The cytotoxicity of 48 compounds included in the MEIC (Multicenter Evaluation of In Vitro Cytotoxicity) list was determined in cultures of rat hepatocytes, McCoy, and MDBK cells. The average minimum concentration of each compound inducing cytotoxicity was measured in each cell type. The cytotoxicity values were then compared with published oral LDS p values for rats and mice. The logarithmic transformation of in vivo toxic doses and the corresponding in vitro cytotoxic concentrations showed a statistically significant correlation between the in vitro and in vivo values. The results show that an accurate in vivo LDS p dose could be predicted from in vitro data for at least 75% of the selected compounds. It is hoped that this finding will not only stimulate others to pursue in vitro technique but will eventually lead to elimination of the in vivo LD50 test.