Summary
Candida shehatae ATCC 22984 and Pichia stipitis CBS 5776 were tested for ethanol production from xylose, glucose-xylose mixtures, and aspen wood total hydrolysates. Adaptation of these yeasts to wood hydrolysate solutions by recycling resulted in improved substrate utilization and ethanol production. Compared to the non-adapted cultures, recycled C. shehatae and P. stipitis in aspen hydrolysate increased g ethanol/g sugar consumed from 0.39 and 0.41 to 0.45 and 0.47; while ethanol production from a 70:30 glucose-xylose solution (total sugars 140 g/L) was 45 g/L in 24 h and 60 g/L in 72 h with the adapted yeasts compared to 15 g/L and 28 g/L in the same times with the parent strains.
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Parekh, S.R., Yu, S. & Wayman, M. Adaptation of Candida shehatae and Pichia stipitis to wood hydrolysates for increased ethanol production. Appl Microbiol Biotechnol 25, 300–304 (1986). https://doi.org/10.1007/BF00253667
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DOI: https://doi.org/10.1007/BF00253667