Summary
A technique based on the use of silver solutions, which selectively stains the nucleolus-organising regions (NORs) in chromosomes, was applied to interphase Ehrlich tumour cells. The results indicate that nucleolar fibrillar centres correspond to the NORs.
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References
Bloom, S.E., Goodpasture, C.: An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes. Hum. Genet. 34, 199–206 (1976)
Bolognari, A., Caminiti, M.: Characteristics of the nucleolini observed under the photon microscope. Experientia 28, 554–556 (1972)
Goessens, G.: Les “centres fibrillaires” des nucléoles de cellules tumorales d'Ehrlich. C. R. Acad. Sci. [D] (Paris) 277, 325–327 (1973)
Goessens, G.: High resolution autoradiographic studies of Ehrlich tumour cell nucleoli. Nucleolar labelling after 3H-actinomycin D binding to DNA or after 3H-TdR or 3H-uridine incorporation in nucleic acids. Exp. Cell Res. 100, 88–94 (1976)
Goessens, G., Lepoint, A.: The fine structure of the nucleolus during interphase and mitosis in Ehrlich tumour cells cultivated in vitro. Exp. Cell Res. 87, 63–72 (1974)
Goodpasture, C., Bloom, S.E.: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma (Berl.) 53, 37–50 (1975)
Hernandez-Verdun, D., Hubert, J., Bourgeois, Cl., Bouteille, M.: Identification ultrastructurale de l'organisateur nucléolaire par la technique à l'argent. C. R. Acad. Sci. [D] (Paris) 287, 1421–1423 (1978)
Howell, W.M., Denton, T.E., Diamond, J.R.: Differential staining of the satellite regions of human acrocentric chromosomes. Experientia 31, 260–262 (1975)
Knibiehler, B., Navarro, A., Mirre, C., Stahl, A.: Localization of ribosomal cistrons in the quail oocyte during meiotic prophase I. Exp. Cell Res. 110, 153–157 (1977)
Lepoint, A., Bassleer, R.: Etude cytologique et cytochimique de cellules tumorales d'Ehrlich de la souris cultivées in vitro. C. R. Acad. Sci. [D] (Paris) 276, 441–443 (1973)
Love, R., Soriano, R.Z.: Correlation of nucleolini with fine structural nucleolar constituents of cultured normal and neoplastic cells. Cancer Res. 31, 1030–1037 (1971)
Schwarzacher, H.G., Mikelsaar, A.V., Schneld, W.: The Nature of the Ag-staining of nucleolus organizer regions. Electron- and light-microscopic studies on human cells in interphase mitosis, and meiosis. Cytogenet. Cell Genet. 20, 24–39 (1978)
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Goessens, G. Localisation of nucleolus-organising regions in interphase cells. Cell Tissue Res. 200, 159–161 (1979). https://doi.org/10.1007/BF00236896
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DOI: https://doi.org/10.1007/BF00236896