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Staphylococcal nuclease reviewed: A Prototypic study in contemporary enzymology. II. Solution studies of the nucleotide binding site and the effects of nucleotide binding.

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Summary

This is the second of a series of four articles in which the chemical, enzymological and crystallographic work on Ribonucleate (deoxyribonucleate)-3′-nucleotidohydrolase EC 3.1.4.4 (staphylococcal nuclease, micrococcal nuclease) will be reviewed and correlated. This article discusses studies in solution delineating the extent of the binding site of the enzyme and identifying some of the particular amino acid residues that form this site. In addition, the effects of the very potent inhibitory combination of thymidine-3′,5′-diphosphate and Ca2+ on the conformation of the enzyme and its physical, chemical and enzymological properties will be reviewed.

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References

  1. Tucker, P. W., Hazen, E. E., Jr., Cotton, F. A., 1977. Mol. Cell. Biochem.

  2. Arnone, A., Bier, C. J., Cotton, F. A., and Hazen, E. E., Jr., Richardson, D. C., Richardson, J. S., Day, V. W., and Yonath, A., 1971. J. Biol. Chem. 246, 2302.

    Google Scholar 

  3. Cuatrecasas, P., Fuchs, S., and Anfinsen, C. B., 1967. J. Biol. Chem. 242,

  4. Cuatrecasas, P., Fuchs, S., and Anfinsen, C. B., 1967. J. Biol. Chem. 242, 1541.

    Google Scholar 

  5. Cuatrecasas, P., Fuchs, S., and Anfinsen, C. B., 1967. J. Biol. Chem. 242, 4759.

    Google Scholar 

  6. Cuatrecasas, P., Edelhoch H., and Anfinsen, C. B., 1967. Proc. Nat. Acad. Sci. U.S., 58, 2043.

    Google Scholar 

  7. Omenn, G. S., Cuatrecasas, P., and Anfinsen, C. B., 1968. Proc. Nat. Acad. Sci., U.S. 64, 923.

    Google Scholar 

  8. Fuchs, S., Cuatrecasas, P., and Anfinsen, C. B., 1969, J. Biol. Chem. 244, 943.

    Google Scholar 

  9. Markley, J. L., Putter, I., and Jardetzky, O., 1968. Science, 161, 1249.

    Google Scholar 

  10. Meadows, D. H., Markley, J. L., Cohen, J. S., and Jardetzky, O., 1967. Proc. Nat. Acad. Sci. U.S.A., 58, 1307.

    Google Scholar 

  11. Markley, J. L., Williams, M. N., and Jardetzky, O., 1970. Proc. Nat. Acad. Sci. U.S.A., 65, 645.

    Google Scholar 

  12. Markley, J. L. and Jardetzky, O., 1970. J. Mol. Biol. 50, 223.

    Google Scholar 

  13. Furie, B., Eastlake, A., Schechter, A. N., and Anfinsen, C. B., 1973. J. Biol. Chem. 248, 5821.

    Google Scholar 

  14. Nieboer, E., East, D., Cohen, J. S., Furie, B., and Schechter, A. N., 1973. Proc. of the Tenth Rare Earth Conference, May 1973, Carefree, Ariz., p.p. 763–769, U.S. Dept of Commerce, Springfield, Virginia.

    Google Scholar 

  15. Taniuchi, H., and Bohnert, J. L., 1975. J. Biol. Chem. 250, 2388.

    Google Scholar 

  16. Dunn, B. M. and Chaiken, I. M., 1975. Biochemistry 14, 2343.

    Google Scholar 

  17. Schechter, I., and Berger, A., 1967. Biochem. Biophys. Res. Comm. 27, 157.

    Google Scholar 

  18. Abramowitz, N. and Schecter, I., 1967. Biochem. Biophys. Res. Comm. 29, 862.

    Google Scholar 

  19. Cuatrecasas, P., Wilchek, M., and Anfinsen, C. B., 1968. Science 62, 1491.

    Google Scholar 

  20. Cuatrecasas, P., Wilcheck, M., and Anfinsen, C. B., 1969. Biochemistry 8, 2277.

    Google Scholar 

  21. Collins, D. M., Cotton, F. A., Hazen, E. E., Jr., and Legg, M. J., 1975. Structure and Conformations of Nucleis Acids and Protein-Nucleic Acid Interactions (Sundaralingam, M., and Rao, S. T., p. 317) ed. University Park Press, Baltimore.

  22. Emerson, T. R., Swan, R. J. and Ulbricht, T. L. V., 1967. Biochemistry 6, 843.

    Google Scholar 

  23. Tucker, P. W., 1975. Ph.D. Dissertation, Texas A&M University.

  24. Parikh, I., and Omenn, G. S., 1970. Biochemistry 10, 1173.

    Google Scholar 

  25. Omenn, G. S., Ontjes, D. A., and Anfinsen, C. B., 1970. Biochemistry 9, 304.

    Google Scholar 

  26. Cuatrecasas, P., Fuchs, S. and Anfinsen, C. B., 1968. Biochim. Biophys. Acta 159, 417.

    Google Scholar 

  27. Cuatrecasas, P., Fuchs, S., and Anfinsen, C. B., 1968. J. Biol. Chem. 243, 4787.

    Google Scholar 

  28. DiBello, C. and Griffin, J. H., 1975. J. Biol. Chem. 250, 1445.

    Google Scholar 

  29. Cuatrecasas, P., Fuchs, S., and Anfinsen, C. B., 1969. J. Biol. Chem. 244, 406.

    Google Scholar 

  30. Cuatrecasas, P., Wilchek, M., and Anfinsen, C. B., 1969. J. Biol. Chem. 244, 4316.

    Google Scholar 

  31. Cuatrecasas, P., 1970. J. Biol. Chem. 245, 574.

    Google Scholar 

  32. Markley, J. L., 1969. Ph.D. Thesis, Harvard University.

  33. Jardetzky, O., Thielmann, H., Arata, Y., Markley, J. L., and Williams, M. N., 1972. Cold Spring Harbor Symp. Quant. Biol. 36, 257.

    Google Scholar 

  34. Anfinsen, C. B. and Corley, L. G., 1969. J. Biol. Chem. 244, 5149.

    Google Scholar 

  35. Chaiken, I. M. and Anfinsen, C. B., 1971. J. Biol. Chem. 246, 2285.

    Google Scholar 

  36. Cuatrecasas, P., Fuchs, S., and Anfinsen, C. B., 1967. J. Biol. Chem. 242, 4759.

    Google Scholar 

  37. Schechter, A. N., Moravek, L., and Anfinsen, C. B., 1968. Proc. Nat. Acad. Sci. U.S.A. 61, 1478.

    Google Scholar 

  38. Cohen, J. S., Shrager, R. I., McNell, M., and Schechter, A. N., 1970. Nature 228, 642.

    Google Scholar 

  39. Cuatrecasas, P., Taniuchi, H., and Anfinsen, C. B., 1969. Brookhaven Symp. Biol. 21, 172.

    Google Scholar 

  40. Markley, J. L., Williams, M. W., and Jardetzky, O., 1970. Proc. Nat. Acad. Sci. U.S.A. 65, 395.

    Google Scholar 

  41. Taniuchi, H., Anfinsen, C. B., and Sodja, A., 1967. Proc. Nat. Acad. Aci. U.S.A. 58, 1235.

    Google Scholar 

  42. Arnone, A., Bier, C. B., Cotton, F. A., Hazen, E. E., Jr., Richardon, D. C., and Richardson, J. S., 1966. Proc. Nat. Acad. Sci. U.S.A. 64, 420.

    Google Scholar 

  43. Sulkowski, E. and Laskowski, M., Sr., 1968. J. Biol. Chem. 243, 4917.

    Google Scholar 

  44. Taniuchi, H. and Anfinsen, C. B., 1966. J. Biol. Chem. 241, 4366.

    Google Scholar 

  45. Taniuchi, H., Anfinsen, C. B., and Sodja, A., 1967. J. Biol. Chem. 242, 4736.

    Google Scholar 

  46. Taniuchi, H., Anfinsen, C. B., and Sodja, A., 1967. J. Biol. Chem. 242, 4752.

    Google Scholar 

  47. Cone, J. L., Cusumano, C. L., Taniuchi, H., and Anfinsen, C. B., 1961. J. Biol. Chem. 246, 3103.

    Google Scholar 

  48. Taniuchi, H., Moravek, L., and Anfinsen, C. B., 1969. J. Biol. Chem. 244, 4600.

    Google Scholar 

  49. Anfinsen, C. B., Cuatrecasas, P., and Taniuchi, H., 1971. The Enzymes (Boyer, P. D., ed.) Vol. 3, 3rd Ed., p. 177, Academic Press, New York.

  50. Taniuchi, H. and Anfinsen, C. B., 1968. J. Biol. Chem. 243, 4778.

    Google Scholar 

  51. Taniuchi, H., Davies, D. R., and Anfinsen, C. B. 1972. J. Biol. Chem. 247, 3362.

    Google Scholar 

  52. Chaiken, I. M., 1971. J. Biol. Chem. 246, 2948.

    Google Scholar 

  53. Sanchez, G. R., Chaiken, I. M., and Anfinsen, C. B., 1973. J. Biol. Chem. 248, 3653.

    Google Scholar 

  54. Taniuchi, H., and Anfinsen, C. B., 1969. J. Biol. Chem 244, 3864.

    Google Scholar 

  55. Taniuchi, H., 1970. Federation Proc. 29, 335.

    Google Scholar 

  56. Parikh, I., Corley, I., and Anfinsen, C. B., 1961. J. Biol. Chem. 246, 7392.

    Google Scholar 

  57. Taniuchi, H., 1963. J. Biol. Chem. 248, 5164.

    Google Scholar 

  58. Anfinsen, C. B., 1963. Science 181, 223.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Genetics, University of Wisconsin-Madison, 53706, Madison, Wisconsin

    Phillip W. Tucker

  2. Department of Chemistry, Texas A&M University, 77843, Texas, College Station, USA

    Edward E. Hazen Jr. & F. Albert Cotton

Authors
  1. Phillip W. Tucker
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  2. Edward E. Hazen Jr.
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  3. F. Albert Cotton
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Additional information

This article is the second of a series of four which provide a comprehensive review of the biochemistry of the staphylococcal nuclease. Part I (1) presented the details of its isolation and enzymology. Part III, in the next issue of this journal, will continue with a review of the three-dimensional structure and mechanism of enzyme action. Part IV will discuss polypeptide chain folding. Work from this laboratory has been supported by grants from the National Institute of General Medical Sciences and the Robert A. Welch Foundation to F. A. Cotton and E. E. Hazen, Jr.

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Tucker, P.W., Hazen, E.E. & Cotton, F.A. Staphylococcal nuclease reviewed: A Prototypic study in contemporary enzymology. II. Solution studies of the nucleotide binding site and the effects of nucleotide binding.. Mol Cell Biochem 23, 3–16 (1979). https://doi.org/10.1007/BF00226675

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  • DOI: https://doi.org/10.1007/BF00226675

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