Skip to main content
Log in

Genetic analysis of soybean hard seededness with molecular markers

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Hard seededness in soybean [Glycine max (L.) Merr.] is a quantitative trait that affects the germination rate, viability, and quality of stored seeds. We have used 72 restriction fragment length polymorphisms (RFLPs) to identify genomic regions containing quantitative trait loci (QTL) affecting hard seededness in a segregating population from a G. max by a Glycine soja (Sieb. & Zucc.) cross. Five independent RFLP markers were found to be associated with variation in the hard-seeded trait. These markers and the epistatic interactions between them explain 71% of the variation for hard seededness. A genomic region associated with the i locus accounted for 32% of the variation in this segregating population. This study illustrates one approach to physiological genetic studies in plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Apuya NA, Frazier B, Keim P, Roth JE, Lark KG (1988) Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) Merrill. Theor Appl Genet 75:889–901

    Google Scholar 

  • Ballard LV (1973) Physical barriers to germination. Seed Sci Technol 1:285–303

    Google Scholar 

  • Bernard R, Weiss MU (1973) Qualitative genetics. In: Caldwell BE (ed) Soybeans: improvement, product, and uses. American Society of Agronomy, Madison/WI, pp 117–164

    Google Scholar 

  • Bowman JL, Yanofsky MF, Meyerowitz EM (1989) Arabidopsis thaliana: A review. Oxford Surv Plant Mol Cell Biol 5 (in press)

  • Burr B, Helentjaris T, Tanksley S (eds) (1989) Development and application of molecular markers to problems in plant genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY

    Google Scholar 

  • Burton J (1987) Quantitative genetics: results relevant to soybean. In: Wilcox JR (ed) Soybeans: improvement, production and uses, 2nd edn. American Society of Agronomy, Madison/WI, pp 211–247

    Google Scholar 

  • Edwards M, Stuber C, Wendell J (1987) Molecular-marker-facilitated investigations of quantitative-trait loci in maize. I. Numbers, genomic distribution, and types of gene action. Genetics 116:113–125

    CAS  PubMed  Google Scholar 

  • Egley GH, Paul RN Jr (1981) Morphological observations on the early imbibition of water by Sida spinosa (Malvaceae) seed. Am J Bot 68:1056–1065

    Google Scholar 

  • Egley GH, Paul RN Jr, Vaugh KC, Duke SO (1983) Role of peroxidase in the development of water-impermeable seed coats in Sida spinosa L. Planta 157:224–232

    Google Scholar 

  • Gots JS, Benson CE (1974) Biochemical genetics of bacteria. Annu Rev Genet 8:79–101

    Google Scholar 

  • Griffin JD (1986) Genetic and germplasm studies with several isozyme loci in soybean [Glycine max (L.) Merr.]. PhD dissertation Iowa State University, Ames, University Microfilms, Ann Arbor/MI, Diss. No. 86–27111

    Google Scholar 

  • Henry SA, Klig LS, Loewy BS (1984) The genetic regulation and coordination of biosynthetic pathways in amino acid and phospholipid synthesis. Annu Rev Genet 18:207–231

    Google Scholar 

  • Keim P, Shoemaker RC (1988) Construction of a random recombinant DNA library that is primarily single-copy sequence. Soybean Genet Newslett 15:147–148

    Google Scholar 

  • Keim P, Olson TC, Shoemaker RC (1988) A rapid protocol for isolating soybean DNA. Soybean Genet Newslett 15:150–152

    Google Scholar 

  • Kilen TC, Hartwig EE (1978) An inheritance study of impermeable seed in soybeans. Field Crops Res 1:65–70

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly M, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Maniatis T, Fritsch EF, Stambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY

    Google Scholar 

  • Marbach I, Mayer AM (1974) Permeability of seed coats to water as related to drying conditions and metabolism of plants. Plant Physiol 54:817–820

    Google Scholar 

  • Marbach I, Mayer AM (1975) Changes in catechol oxidase and permeability to water in seed coats of Pisum elatius during seed coat development and maturation. Plant Physiol 56:93–96

    Google Scholar 

  • Nienhuis J, Helentjaris T, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci 27:797–803

    Google Scholar 

  • Palmer RG, Kilen TC (1987) Qualitative genetics and cytogenetics. In: Wilcox JR (ed) Soybeans: improvement, production, and uses. 2nd edn. American Society of Agronomy, Madison/WI, pp 135–209

    Google Scholar 

  • Potts HC, Duangpatra J, Hairston WG, Delouche JC (1978) Some influences of hard seededness on soybean quality. Crop Sci 18:221–224

    Google Scholar 

  • Rolston MP (1978) Water impermeable seed dormancy. Bot Rev 44:365–396

    Google Scholar 

  • Starzing EK, West SH, Hinson K (1982) An observation on the relationship of soybean seed color to viability maintenance. Seed Sci Technol 10:301–305

    Google Scholar 

  • Tanksley SD, Hewitt J (1988) Use of molecular markers in breeding for soluble solids content in tomato — a re-examination. Theor Appl Genet 75:811–823

    Google Scholar 

  • Terzaghi W, Roth EJ, Keim P, Honeycutt EJ, Lark KG (1985) A somatic genetics system for soybean. In: Freeling M (ed) Plant genetics, UCLA Symposia on Molecular and Cellular Biology, New Series 35:771–789

  • Yaklich RW, Vigil EL, Wergin WP (1984) Scanning electron microscopy of soybean seed coats. Scanning Electron Microsc 2:991–1000

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by K. Tsunewaki

Journal Paper No. J-13557 of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA, Project No. 2763

Rights and permissions

Reprints and permissions

About this article

Cite this article

Keim, P., Diers, B.W. & Shoemaker, R.C. Genetic analysis of soybean hard seededness with molecular markers. Theoret. Appl. Genetics 79, 465–469 (1990). https://doi.org/10.1007/BF00226154

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226154

Key words

Navigation