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Studies of cardiac ganglia in pre- and postnatal rabbits

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Summary

This investigation was undertaken to describe the ultrastructure of cardiac ganglia in rabbits from day 18 of gestation to day 35 postpartum. Special attention was directed to the types of synaptic contacts made with the principal neurons and with the small granule-containing cells. The cardiac ganglia in all animals consisted mainly of parasympathetic postganglionic neurons, supporting cells, and small granule-containing (small intensely fluorescent) cells. The neurons received afferent synaptic terminals of two types. One type contained mainly small clear vesicles typical of most cholinergic terminals. The second type contained mainly small dense-core vesicles (these were most prominent after treatment of the animal with 5-hydroxydopamine), and were considered to be adrenergic terminals. These adrenergic terminals are probably part of an inhibitory system in the ganglia. The small granule-containing cells received typical afferent synaptic terminals of the cholinergic type, and also formed specialized contacts with certain axonal terminals. These latter specializations are considered to be reciprocal synapses which probably have a role in modulating ganglionic transmission.

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References

  • Björklund, A., Cegrell, L., Falck, B., Ritzen, M., Rosengren, E.: Dopamine-containing cells in sympathetic ganglia. Acta physiol. scand. 78, 334–338 (1970)

    Google Scholar 

  • Cantino, D., Mugnaini, E.: Adrenergic innervation of the parasympathetic ciliary ganglion in the chick. Science 185, 279–281 (1974)

    Google Scholar 

  • Curds, D.R.: The pharmacology of central and peripheral inhibition. Pharmacol. Rev. 15, 333–364 (1963)

    Google Scholar 

  • Dail, W.G. Jr., Evan, A.P. Jr., Eason, H.R.: The major pelvic ganglion in the pelvic plexus of the male rat. A histochemical and ultrastructural study. Cell Tiss. Res. 159, 49–62 (1975)

    Google Scholar 

  • Ehinger, B.: Adrenergic nerves in the avian eye and ciliary ganglion. Z. Zellforsch. 82, 577–588 (1967)

    Google Scholar 

  • Ehinger, B., Falck, B., Persson, H., Sporrong, B.: Adrenergic and cholinesterase-containing neurons of the heart. Histochemie 16, 197–205 (1968)

    Google Scholar 

  • Elfvin, L.G., Hökfelt, T., Goldstein, M.: Fluorescence microscopical, immunohistochemical and ultrastructural studies on sympathetic ganglia of the guinea-pig, with special reference to the SIF cells and their catecholamine content. J. Ultrastruct. Res. 51, 377–396 (1975)

    Google Scholar 

  • Ellison, J.P., Hibbs, R.G.: An ultrastructural study of mammalian cardiac ganglia. J. Molec. Cell. Cardiol. 8, 89–101 (1976)

    Google Scholar 

  • Ellison, J.P., Olander, K.W.: Simultaneous demonstration of catecholamines and acetylcholinesterase in peripheral autonomic nerves. Amer. J. Anat. 135, 23–32 (1972)

    Google Scholar 

  • Eränkö, L.: Ultrastructure of the developing sympathetic nerve cell and the storage of catecholamines. Brain Res. 46, 159–175 (1972)

    Google Scholar 

  • Falck, B., Owman, Ch.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic amines. Acta Univ. Lund., Sect. II, 7, 1–23 (1965)

    Google Scholar 

  • Gabella, G.: Fine structure of the myenteric plexus in the guinea-pig ileum. J. Anat. (Lond.) 111, 69–97 (1972)

    Google Scholar 

  • Hervonen, H.: Histochemical and electron microscopical study on sympathetic ganglia of chick embryo in culture. Acta Inst. Anat. Univ. Helsinkiensis, Supp. 8, 1–35 (1975)

    Google Scholar 

  • Ivanov, D.P.: Recherches ultrastructurales sur les cellules paraganglionnaires du ganglion coeliaque du rat et leurs connexions avec les neurones. Acta anat. (Basel) 89, 266–286 (1974)

    Google Scholar 

  • Jacobowitz, D.: Histochemical studies of the autonomic innervation of the gut. J. Pharmacol. exp. Ther. 149, 358–364 (1965)

    Google Scholar 

  • Jacobowitz, D.: Histochemical studies of the relationship of chromaffin cells and adrenergic nerve fibers to the cardiac ganglia of several species. J. Pharmacol. exp. Ther. 158, 227–240 (1967)

    Google Scholar 

  • Jacobowitz, D., Kent, K.M., Fleisch, J.H., Cooper, T.: Histofluorescent study of catecholamine-containing elements in cholinergic ganglia from the calf and dog lung. Proc. Soc. exp. Biol. (N.Y.) 144, 464–466 (1973)

    Google Scholar 

  • Jacobowitz, D., Nemir, P.: The autonomic innervation of the esophagus of the dog. J. Thorac. cardiovasc. Surg. 58, 678–684 (1969)

    Google Scholar 

  • Kanerva, L.: Ultrastructure of sympathetic ganglion cells and granule-containing cells in the paracervical (Frankenhäuser) ganglion of the newborn rat. Z. Zellforsch. 126, 25–40 (1972a)

    Google Scholar 

  • Kanerva, L.: Light and electron microscopic observations on the postnatal development of the rat paracervical (Frankenhäuser) ganglion. Z. Anat. Entwickl.-Gesch. 136, 33–50 (1972b)

    Google Scholar 

  • Kanerva, L., Teräväinen, H.: Electron microscopy of the paracervical (Frankenhäuser) ganglion of the adult rat. Z. Zellforsch. 129, 161–177 (1972)

    Google Scholar 

  • Kebabian, J.W., Greengard, P.: Dopamine-sensitive adenylcyclase: Possible role in synaptic transmission. Science 174, 1346–1348 (1971)

    Google Scholar 

  • Libet, B.: Generation of slow inhibitory and excitatory postsynaptic potentials. Fed. Proc. 29, 1945–1956 (1970)

    Google Scholar 

  • Libet, B., Owman, Ch.: Concomitant changes in formaldehyde-induced fluorescence of dopamine interneurons and in slow inhibitory postsynaptic potentials of the rabbit superior cervical ganglion, induced by stimulation of the preganglionic nerve or by a muscarinic agent. J. Physiol. (Lond.) 237, 635–662 (1974)

    Google Scholar 

  • Libet, B., Tosaka, T.: Dopamine as a synaptic transmitter and modulator in sympathetic ganglia: A different mode of synaptic action. Proc. nat. Acad. Sci. 67, 667–673 (1970)

    Google Scholar 

  • Matthews, M.R., Raisman, G.: The ultrastructure and somatic efferent synapses of small granule-containing cells in the superior cervical ganglion. J. Anat. (Lond.) 105, 255–282 (1969)

    Google Scholar 

  • Navartnam, V., Lewis, P.R., Schute, C.C.D.: Effects of vagotomy on the cholinesterase content of the preganglionic innervation of the rat heart. J. Anat. (Lond.) 103, 225–232 (1968)

    Google Scholar 

  • Nielsen, K.C., Owman, Ch.: Difference in cardiac adrenergic innervation between hibernators and non-hibernating mammals. Acta physiol. scand. (Suppl. 316) 1–30 (1968)

    Google Scholar 

  • Nishi, S.: Cholinergic and adrenergic receptors at sympathetic preganglionic nerve terminals. Fed. Proc. 29, 1957–1965 (1970)

    Google Scholar 

  • Norberg, K.-A.: Adrenergic innervation of the intestinal wall studied by fluorescence microscopy. Int. J. Neuropharmacol. 3, 279–382 (1964)

    Google Scholar 

  • Norberg, K.-A.: Transmitter histochemistry of the sympathetic adrenergic nervous system. Brain Res. 5, 125–170 (1967)

    Google Scholar 

  • Norberg, K.-A., Sjoqvist, F.: New possibilities for adrenergic modulation of ganglionic transmission. Pharmacol. Rev. 18, 743–751 (1966)

    Google Scholar 

  • Osborne, L.W., Silva, D.G.: Histological, acetylcholinesterase, and fluorescence histochemical studies on the atrial ganglia of the monkey heart. Exp. Neurol. 27, 497–511 (1970)

    Google Scholar 

  • Papka, R.E.: Ultrastructural and fluorescence histochemical studies of developing sympathetic ganglia in the rabbit. Amer. J. Anat. 134, 337–364 (1972)

    Google Scholar 

  • Papka, R.E.: A study of catecholamine-containing cells in the hearts of fetal and postnatal rabbits by fluorescence and electron microscopy. Cell Tiss. Res. 154, 471–484 (1974)

    Google Scholar 

  • Richardson, K.C.: The fine structure of the albino rabbit iris with special reference to the identification of adrenergic and cholinergic nerves and nerve endings in its intrinsic muscles. Amer. J. Anat. 114, 173–206 (1964)

    Google Scholar 

  • Siegrist, G., Dolivo, M., Dunant, Y., Foroglou-Kerameus, D., de Ribaupierre, Fr., Rouiller, Ch.: The ultrastructure and function of the chromaffin cells in the superior cervical ganglion of the rat. J. Ultrastruct. Res. 25, 381–407 (1968)

    Google Scholar 

  • Taxi, J., Gautron, J., L'Hermite, P.: Données ultrastructurales sur une eventuelle modulation adrenergique de l'activite du ganglion cervical superieur du rat. C. R. Acad. Sci. (Paris) D 269, 1281–1284 (1969)

    Google Scholar 

  • Topchieva, E.P.: Electrical responses in individual neurons in intramural ganglia of frog heart to stimulation of vagus and sympathetic nerves. Fed. Proc. (trans. suppl.) 25, T739-T742 (1966)

    Google Scholar 

  • Trendelenburg, U.: Pharmacology of autonomic ganglia. Ann. Rev. Pharmacol. 1, 219–238 (1961)

    Google Scholar 

  • Volle, R.L.: Modification by drugs of synaptic mechanisms in autonomic ganglia. Pharmacol. Rev. 18, 839–870 (1966)

    Google Scholar 

  • Watanabe, H.: Adrenergic nerve elements in the hypogastric ganglion of the guinea pig. Amer. J. Anat. 130, 305–330 (1971)

    Google Scholar 

  • Williams, T.H., Palay, S.L.: Ultrastructure of the small neurons in the superior cervical ganglion. Brain Res. 15, 17–34 (1969)

    Google Scholar 

  • Yamauchi, A., Fujimaki, Y., Yokota, R.: Reciprocal synapses between cholinergic postganglionic axon and adrenergic interneuron in the cardiac ganglion of the turtle. J. Ultrastruct. Res. 50, 47–57 (1974)

    Google Scholar 

  • Yamauchi, A., Yokota, R., Fujimaki, Y.: Reciprocal synapses between cholinergic axons and small granule-containing cells in the rat cardiac ganglion. Anat. Rec. 181, 195–210 (1975)

    Google Scholar 

  • Yokota, R., Yamauchi, A.: Ultrastructure of the mouse superior cervical ganglion, with particular reference to the pre- and postganglionic elements covering the soma of its principal neurons. Amer. J. Anat. 140, 281–298 (1974)

    Google Scholar 

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  1. Department of Anatomy, University of Kentucky Medical Center, Lexington, Kentucky, USA

    Raymond E. Papka

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  1. Raymond E. Papka
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Supported by the Kentucky Heart Association and the Heart Association of Louisville and Jefferson County

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Papka, R.E. Studies of cardiac ganglia in pre- and postnatal rabbits. Cell Tissue Res. 175, 17–35 (1976). https://doi.org/10.1007/BF00220820

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