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Immunoelectron microscopic evidence of contractile proteins in the cellular and acellular components of mouse kidney glomeruli

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Summary

Actin and/or actin-like protein have been localized in the cellular and acellular components of the glomerular walls of mouse kidney by means of immunoelectron microscopy, employing human antibodies to smooth muscle (SMA).

Contractile antigens have been confirmed to be present in the cytoplasm of podocytes and mesangial cells in association with fine filaments which are considered of importance in the control of blood flow, intravascular pressure, and filtration rate within the glomerulus. The extracellular presence of contractile proteins in the mesangial matrix and glomerular basement membrane can be related to cell movement in a frictional environment. This latter phenomenon, which is strictly interdependent with cell adhesion and aggregation, is most evident in the mesangial cells in a form of luminar pseudopodia, cytoplasmic projections, and phagocytosis.

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References

  • Accinni, L., Hsu, K. C., Spiele, H., De Martino, C.: Picric acid-formaldehyde fixation for immunoferritin studies. Histochemistry 42, 257–264 (1974)

    Google Scholar 

  • Andres, G. A., Hsu, K. C., Seegal, B. C.: Immunologic techniques for identification of antigens or antibodies by electron microscopy. In: Handbook of experimental immunology, chap. 34. Oxford: Blackwell Sci. Publ. 1973

    Google Scholar 

  • Arakawa, M., Kimmelstiel, P.: Circumferential mesangial interposition. Lab. Invest. 21, 276–284 (1969)

    Google Scholar 

  • Barajas, L., Latta, H.: A three-dimensional study of the juxtaglomerular apparatus in the rat: light and electron microscopic observation. Lab. Invest. 12, 257–269 (1963)

    Google Scholar 

  • Bargmann, W: Über Structur und Speicherungsvermögen des Nierenglomerulus. Z. Zellforsch. 14, 73–137 (1932)

    Google Scholar 

  • Becker, C. G.: Demonstration of actomyosin in mesangial cells of the renal glomerulus. Amer. J. Path. 66, 97–110 (1972)

    Google Scholar 

  • Bernik, M.: Contractile activity of human glomeruli in culture. Nephron 6, 1–10 (1969)

    Google Scholar 

  • Churg, J., Grishman, E., Goldstein, M. H., Yunis, S. D., Porush, J. G.: Idiopathic nephrotic syndrome in adults. New Engl. J. Med. 272, 165–183 (1965)

    Google Scholar 

  • De Martino, C., Accinni, L., Procicchiani, G.: Ultrastructural study on contractile structures in mammalian nephron. Their development in the metanephros of human embryo. Z. Zellforsch. 140, 101–124 (1973)

    Google Scholar 

  • De Martino,C., Picardi, R., Procicchiani, G.: Présence de structures contractiles dans différents segments du néphron chez les mammifères. J. Microscopie 8, 45a (1969)

  • De Martino, C., Zamboni, L.: A morphologic study of the mesonephros of the human embryo. J. Ultrastruct. Res. 16, 399–427 (1966)

    Google Scholar 

  • De Winiwarter, H.: Recherches sur l'histogénèse du glomérule renal. Arch. Biol. (Liège) 24, 207–219 (1943)

    Google Scholar 

  • Dunihue, F. W., Boldosser, W. G.: Observations on the similarity of mesangial to juxta-glomerular cells. Lab. Invest. 12, 1228–1240 (1963)

    Google Scholar 

  • Faith, G. C., Trump, B. F.: The glomerular capillary wall in human kidney disease. Acute glomerulonephritis, systemic lupus erythematosus, and pre-eclampsia eclampsia. Lab. Invest. 15, 1682–1719 (1966)

    Google Scholar 

  • Farquhar, M. G., Palade, G.: Functional evidence for the existance of a third cell type in the renal glomerulus. J. Cell Biol. 13, 55–87 (1962)

    Google Scholar 

  • Farquhar, M. G., Wissig, S. L., Palade, G. E.: Glomerular permeability. I. Ferritin transfer across the normal glomerular capillary wall. J. exp. Med. 113, 47–66 (1961)

    Google Scholar 

  • Farrow, L. J., Holborow, E. J., Brighton, W. D.: Reaction of human smooth muscle antibody with liver cells. Nature (Lond.) New Biol. 232, 186–187 (1971)

    Google Scholar 

  • Farrow, L. J., Holborow, E. J., Johnson, G. D., Lamb, S. G., Stewart, J. S., Taylor, P. E., Zuckerman, A. J.: Autoantibodies and the hepatitis-associated antigen in acute infective hepatitis. Brit. med. J. 2, 693–695 (1970)

    Google Scholar 

  • Fagraeus, A., Lidman, K., Biberfeld, G.: Reaction of human smooth muscle antibodies with human blood lymphocytes and lymphoid cell lines. Nature (Lond.) 252, 246–247 (1974)

    Google Scholar 

  • Fagraeus, A., The, H., Biberfeld, G.: Reaction of human smooth muscle antibody with thymus medullary cells. Nature (Lond.) New Biol. 246, 113–115 (1973)

    Google Scholar 

  • Gabbiani, G., Ryan, G. B., Badonnel, M. C., Majno, G.: Smooth muscle antigens in platelets: immunofluorescent detection using human anti-smooth muscle serum. Path. Biol. 20 (Suppl.), 6–8 (1972)

    Google Scholar 

  • Gabbiani, G., Ryan, G. B., Lamelin, J. P., Vassalli, P., Majno, G., Bouvier, C. A., Cruchaud, A., Lüscher, E. F.: Human smooth muscle autoantibody. Its identification as antiactin antibody and a study of its binding to “nonmuscular” cells. Amer. J. Path. 72, 473–489 (1973)

    Google Scholar 

  • Goldman, R. D., Lazarides, E., Pollack, R., Weber, K.: The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells. Exptl. Cell Res. 90, 333–344 (1975)

    Google Scholar 

  • Goormaghtigh, N.: La fonction endocrine des artérioles rénales. Son rôle dans la pathogénie de l'hypertension artérielle, p. 1–110. Fontain, Louvain 1942

  • Goormaghtigh, N.: La fonction endocrine des artérioles rénales. Rev. belge Sci. méd. 16, 1–155 (1944)

    Google Scholar 

  • Goormaghtigh, N.: Facts in favor of an endocrine function of the renal arterioles. J. Path. Bact. 57, 392–408 (1945)

    Google Scholar 

  • Gröschel-Stewart, U., Jones, B. M., Kemp, R. B.: Detection of actomyosin-type protein at the surface of dissociated embryonic chick cells. Nature (Lond.) 227, 280 (1970)

    Google Scholar 

  • Harper, J. T., Puchtler, H., Meloan, S. N., Terry, M. S.: Light-microscopic demonstration of myoid fibrils in renal epithelial, mesangial and interstitial cells. J. Microscopie 91, 71–85 (1970)

    Google Scholar 

  • Hirschel, B. J., Gabbiani, G., Rayn, G. B., Majno, G.: Fibroblasts of granulation tissue: immunofluorescent staining with anti-smooth muscle serum. Pro. Soc. exp. Biol. (N.Y.) 138, 466–469 (1971)

    Google Scholar 

  • Holborow, E. J.: Immunological aspects of viral hepatitis. Brit. med. Bull. 28, 142–144 (1972)

    Google Scholar 

  • Holborow, E. J., Hemsted, E. H., Mead, S. V.: Smooth muscle autoantibodies in infectious mononucleosis. Brit. med. J. 3, 323–325 (1973)

    Google Scholar 

  • Huhn, D., Steiner, J. W., Movat, H. Z.: Die Feinstruktur des Mesangium und Nierenglomerulum von Hund und Maus. Z. Zellforsch. 56, 213–230 (1962)

    Google Scholar 

  • Ishii, Y.: An ultrastructural study of the intrauterine development of rabbit metanephric glomeruli. Sapporo med. J. 37, 53–78 (1970)

    Google Scholar 

  • Johnson, G. D., Holborow, E. J., Glynn, L. E.: Antibody to smooth muscle in patients with liver disease. Lancet 1965 II, 878–879

    Google Scholar 

  • Johnson, G. D., Holborow, E. J., Glynn, L. E.: Antibody to liver lupoid hepatitis. Lancet 1966 II, 416–418

    Google Scholar 

  • Jokelainen, P.: An electron microscope study of early development of the rat metanephric nephron. Acta anat. (Basel) 52 (Suppl. 47) (1963)

    Google Scholar 

  • Jones, B. M.: A unifying hypothesis of cell adhesion. Nature (Lond.) 212, 362–365 (1966)

    Google Scholar 

  • Jones, B. M., Kemp, R. B.: Aggregation and electrophoretic mobility studies on dissociated cells. II. Effects of ADP and ATP. Exp. Cell Res. 63, 301–308 (1970)

    Google Scholar 

  • Jones, B. M., Kemp, R. B., Gröschel-Stewart, U.: Inhibition of cell aggregation by antibodies directed against actomyosin. Nature (Lond.) 226, 261–262 (1970)

    Google Scholar 

  • Jones, D. B.: Nature of scar tissue in glomerulonephritis. Amer. J. Path. 42, 185–199 (1963)

    Google Scholar 

  • Jones, P. T. C.: A contractile protein model for cell adhesion. Nature (Lond.) 212, 365–369 (1966)

    Google Scholar 

  • Kemp, R. B., Jones, B. M., Gröschel-Stewart, U.: Aggregative behaviour of embryonic chick cells in the presence of antibodies directed against actomyosin. J. Cell Sci. 9, 103–122 (1971)

    Google Scholar 

  • Kimmelstiel, P., Osawa, G., Beres, J.: Some glomerular changes by electron microscopy with predominant mesangial reaction. In: Proceedings Third International Congress of Nephrology, Washington 1966. New York: Karger 1967

    Google Scholar 

  • Knight, V. A., Jones, B. M., Jones, P. C. T.: Inhibition of the aggregation of dissociated embryo-chick fibroblast cells by adenosine triphosphate. Nature (Lond.) 210, 1008–1010 (1966)

    Google Scholar 

  • Latta, H.: The glomeralar capillary wall. J. Ultrastruct. Res. 32, 526–544 (1970)

    Google Scholar 

  • Latta, H., Maunsbach, A. B., Cook, M. L.: Relations of the centrolobular region of the glomerulus to the juxtaglomerular apparatus. J. Ultrastruct. Res. 6, 562–578 (1962)

    Google Scholar 

  • Lazarides, E., Weber, K.: Actin antibody: the specific visualization of actin filaments in non muscle cells. Proc. nat. Acad. Sci. (Wash.) 71, 2268–2272 (1974)

    Google Scholar 

  • McManus, J. F. A.: The myoid nature of the cells covering the human renal glomerulus. Amer. J. Path. 30, 640 (1954)

    Google Scholar 

  • Michielsen, P.: Contribution a l'étude de tissue intercapillaire. First Int. Congr. of Nephrology (Riehet G., ed.), p. 657. Basel: S. Karger 1961

    Google Scholar 

  • Michielsen, P, Creemers, J.: The structure and function of the glomerular mesangium. In: The ultrastructure of the kidney (A. J. Dalton and F. Haguenau, eds.), p. 57. New York: Academic Press 1967

    Google Scholar 

  • Nagle, R. B., Kneiser, M.R., Bulger, R. E., Benditt, E. P.: Induction of smooth muscle characteristics in renal interstitial fibroblasts during obstructive nephropathy. Lab. Invest. 29, 422–427 (1973)

    Google Scholar 

  • Neifakh, S. A., Avramov, J. A., Gaitskhoki, V. S., Kazakova, T. B., Monakhov, N. K., Repin, V. S., Turovski, V. S., Vassiletz, I. M.: Mechanism of the controlling function of mitrochondria. Biochim. biophys. Acta (Amst.) 100, 329–343 (1965)

    Google Scholar 

  • Newstead, J. D.: Filaments in renal parenchymal and interstitial cells. J. Ultrastruct. Res. 34, 316–328 (1971)

    Google Scholar 

  • Oberling, C., Hatt, P. Y.: Etude de l'appareil juxtaglomérulaire du rat au microscope électronique. Ann. Anat. path. 5, 441–459 (1960)

    Google Scholar 

  • Pease, D. C.: Myoepithelial characteristics of capsular and tubular cells of the kidney cortex. J. Cell Biol. 39, 2. 2, Abs. 249 (1968a)

    Google Scholar 

  • Pease, D. C.: Myoid features of renal corpuscle and tubules. J. Ultrastruct. Res. 23, 304–320 (1968b)

    Google Scholar 

  • Perdue, J. F.: The distribution, ultrastructure, and chemistry of microfilaments in cultured chick embryo fibroblasts. J. Cell Biol. 58, 265–283 (1973)

    Google Scholar 

  • Policard, A., Collet, A., Giltaire-Ralyte, L.: Recherches au microscope électronique sur la structure du glomérule rénal des mammifères. Arch. Anat. micr. Morph. exp. 44, 1–19 (1955)

    Google Scholar 

  • Ross, M. H., Reith, F. J.: Myoid elements in the mammalian nephron and their relationship to other specializations in the basal part of kidney tubule cells. Amer. J. Anat. 129, 399–416 (1970)

    Google Scholar 

  • Rostgaard, J., Kristensen, B. I., Nielsen, L. E.: Electron microscopy of filaments in the basal part of rat kidney tubule cells and their “in situ” interaction with heavy meromyosin. Z. Zellforsch. 132, 497–521 (1972)

    Google Scholar 

  • Rostgaard, J., Thuneberg, L.: Electron microscopic evidence suggesting a contractile system in the tubular cells of rat kidney. J. Ultrastruct. Res. 29, 570 (1969)

    Google Scholar 

  • Rostgaard, J. Thuneberg, L.: Electron microscopical observations on the brush border of proximal tubule of mammalian kidney. Z. Zellforsch. 132, 473–496 (1972)

    Google Scholar 

  • Scheinman, J. I., Fish, A. J., Michael, A. F: The immunohistopathology of glomerular antigens. The glomerular basement membrane, collagen, and actomyosin antigens in normal and diseased kidneys. J. clin. Invest. 54, 1144–1154 (1974)

    Google Scholar 

  • Simon, G. T., Chatelanat, F.: Ultrastructure of the normal and pathological glomerulus. In: The kidney. Morphology, biochemistry, physiology (Rouiller, C., and Muller, A. F. eds.), p. 261. New York and London: Academic Press 1969

    Google Scholar 

  • Sternberg, S. S.: Cross-striated fibrils and other ultrastructural alterations in glomeruli of rats with daunomycin nephrosis. Lab. Invest. 23, 39–51 (1970)

    Google Scholar 

  • Suzuki, Y.: An electron microscopy of the renal differentiation. II. Glomerulus. Keio J. Med. 8, 129–144 (1959)

    Google Scholar 

  • Suzuki, Y., Churg, J., Grishman, E., Mautner, W., Dachs, S.: The mesangium of the renal glomerulus. Electron microscopic studies of pathologic alterations. Amer. J. Path. 43, 555–567 (1963)

    Google Scholar 

  • Tan, E. M.: An immunologic precipitin system between soluble nucleoprotein and serum antibody in systemic lupus erythematosus. J. clin. Invest. 46, 735–745 (1967)

    Google Scholar 

  • Trenchev, O., Sneyd, P., Holborow, E. J.: Immunofluorescent tracing of smooth contractile protein antigens in tissues other than smooth muscle. Clin. exp. Immunol. 16, 125–136 (1974)

    Google Scholar 

  • Whitehouse, J. M. A., Holborow, E. J.: Smooth muscle antibody in malignant disease. Brit. med. J. 4, 511–513 (1971)

    Google Scholar 

  • Whittingham, S., Mackay, I. R., Irwin, J.: Autoimmune hepatitis. Immunofluorescence reactions with cytoplasm of smooth muscle and renal glomerular cells. Lancet 1966, I, 1333–1335

  • Willingham, M. C., Ostlund, R. E., Pastan, I: Myosin is a component of the cell surface of cultured cells. Proc. nat. Acad. Sci. (Wash.) 71, 4144–4148 (1974)

    Google Scholar 

  • Yamada, E.: The fine structure of the renal glomerulus of the mouse. J. biophys. biochem. Cytol. 1, 551–556 (1955)

    Google Scholar 

  • Zamboni, L., De Martino, C.: A re-evaluation of the “mesangial” cells. Proceedings sixth Int. Congr. Electr. Microsc. (R. Uyeda ed.), vol. 2, p. 671. Tokyo: Maruzen 1966

    Google Scholar 

  • Zamboni, L., De Martino, C.: A re-evaluation of the mesangial cells of the renal glomerulus. Z. Zellforsch. 86, 364–383 (1968a)

    Google Scholar 

  • Zamboni, L., De Martino, C.: Embryogenesis of the human renal glomerulus. I. A histologic study. Arch. Path. 86, 279–291 (1968b)

    Google Scholar 

  • Zimmermann, K. W.: Über den Bau des Glomerulus der Säugerniere. Weitere Mitteilungen. Z. mikr.-anat. Forsch. 32, 176–278 (1933)

    Google Scholar 

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Authors and Affiliations

  1. C.N.R. Clinica Medica 2, University of Rome, Rome, Italy

    Lidia Accinni

  2. Regina Elena Institute for Cancer Research, Rome, Italy

    P. G. Natali, Laura Vassallo & C. De Martino

  3. Department of Microbiology, Columbia University, New York, USA

    K. S. Hsu

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  1. Lidia Accinni
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  2. P. G. Natali
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  3. Laura Vassallo
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  4. K. S. Hsu
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  5. C. De Martino
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Accinni, L., Natali, P.G., Vassallo, L. et al. Immunoelectron microscopic evidence of contractile proteins in the cellular and acellular components of mouse kidney glomeruli. Cell Tissue Res. 162, 297–312 (1975). https://doi.org/10.1007/BF00220176

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