Summary
The immunocytochemical study on the ultrastructural localization of human-type ABO(H)-activities in a crab-eating macaque (Macaca irus) was carried out by using postembedding and immuno-gold staining method. The tissue specimens examined were the esophagus, stomach (St), small intestine (Si), large intestine, liver, kidney, and pancreas. The specimens from these organs and submandibular gland (Sg) of a human (O-group) were used as staining reaction controls. Primary and secondary antibodies were commercially obtained mouse monoclonal anti-A, -B, -H (IgM), and goat anti-mouse IgM labeled with colloidal gold particles (Ø 20 nm), respectively. The results were as follows: (1) In macaque specimens, only A-activity could be observed as the location of gold particles on the peripheral rim of serous secretory granules (Sg) and of epithelial cells (esophagus), the mucous droplets in epithelial cells and brush border (St, Si), the intracellular secretory canaliculi [ISC (St)] and the zymogen granules and secretory ducts (pancreas). Gold particles could be also noted at the Golgi apparatus and nascent secretory granules. (2) By periodic acid-thiocarbohydrazide-osmium tetroxide (PA-TCH-OS) reaction, hexose-rich neutral mucopolysaccharides were noted on the peripheral rim of serous secretory granules (Sg), the mucous droplets (St, Si), the ISC (St), and the brush border (Si). Such a distributional pattern corresponded well with that of gold particles, indicating that the substances were responsible for ABO(H)-activities.
Zusammenfassung
Eine immunhistochemische Untersuchung von ultrastrukturellen Lokalisierungen der ABO(H)-Aktivitäten in den Zellen eines krabbenfressenden Affens (Macaca irus) wurde mittels der Post-Einbettung und Immun-Gold-Methode durchgeführt. Die Gewebeproben waren Oesophagus, Magen (Mag.), Dünndarm (Dün.), Dickdarm, Leber, Niere und Pankreas. Humane Proben von diesen Organen und der Glandula submandibularis (Gs) eines Mannes (O-Gruppe) wurden auch als Färbungskontrollen benutzt. Der primäre Antikörper war der käuflich erworbene monoklonale Antikörper gegen A, B oder H (IgM), und der sekundäre Antikörper war mit kolloidalem Gold markiertes Anti-Maus-IgM von der Ziege. Die Ergebnisse waren wie folgt: (1) In den Gewebeproben von Macaca wurde nur A-Aktivität am Rand der Sekretgranula der serösen Speicheldrüsenzellen (Gs) und der Epithelzellen des Oesophagus, in den mukoiden Sekretkörnchen der oberflächlichen Zellen und am Stäbchensaum (Mag., Dün.), an den intrazellulären Sekretkanälchen mit Mikrovilli [ISK (Mag.)] und in den Zymogengranula und den Sekretkanälen (Pankreas) beobachtet. Die Goldkörnchen wurden auch am Golgi-Apparat und an den neugebildeten Sekretkörnchen bemerkt. (2) Mit Hilfe der Perjodsäure-Thiokarbohydrazid-Osmiumtetroxyd (PS-TKH-OS)-Reaktion wurden Hexose-reiche, neutrale Mukopolysaccharide am Rand von Sekretgranula (Gs), in der mukoiden Sekretkörnchen (Mag., Dün.), und an der ISK und dem Stäbchensaum dargestellt. Ihre Lokalisation haben mit derjenigen von Goldkörnchen übereingestimmt. Diese Befunde zeigen, daß die ABO(H)-Aktivitäten diesen Substanzen entsprechen.
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Supported in part by Grant from the Ministry of Education, Science and Culture of Japan, and by the Cooperation Research Program of the Primate Research Institute of Kyoto University, and was presented in part at the 8th Chubu District Medico-Legal Conference (Kanazawa, October 1986) and at the 71st Conference of the Medico-Legal Society of Japan (Tokyo, April 1987)
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Ohshima, T., Maeda, H., Tanaka, N. et al. Immunocytochemical study on the ultrastructural localization of human-type ABO(H)-blood group activities in a macaque (Macaca irus) . Z Rechtsmed 100, 139–148 (1988). https://doi.org/10.1007/BF00200754
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DOI: https://doi.org/10.1007/BF00200754