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Uptake of jasmonic acid and related compounds by mesophyll protoplasts of the barley leaf

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Abstract

Jasmonic acid (JA) permeates the plasma membrane of mesophyll cells by diffusion as the lipophilic undissociated JAH molecule probably without the participation of a saturable uptake component. The mesophyll plasma membrane is nearly impermeable to the JA anion. The permeability coefficients of JA and several JA derivatives (its methyl ester (JAMe), 7-iso-cucurbic acid (7-iso-CA), 6-epi-7-iso-cucurbic acid (6-epi-7-iso-CA), and both stereoisomers of the JA leucine conjugate ((+)-JA-Leu and (-)-JA-Leu)) were determined and used in a simplified mathematical model to predict stressdependent JA redistribution between cytosol and apoplast in comparison with ABA. The redistribution of JA takes place similar to ABA; however, its velocity is much higher because of the high JA membrane permeability. When the permeability coefficients for the mesophyll plasma membrane are plotted double-logarithmically against the ratio of the distribution coefficient and the molecular ratio to the power of 1.5 (KDMr −1.5), two straight lines result for two different classes of compounds. The permeability coefficients of JA conjugates are lower than that of the free acid by approximately one order of magnitude, but they are still significantly higher than that of ABA.

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References

  • Baier M (1990) Transport und Verteilung von Abscisinsäure im Blatt. Die Rolle der Schlie-Bzellen. Dissertation, University of Würzburg, Würzburg

    Google Scholar 

  • Böhmer B, Kiewnick L (1989) Lasiodiplodia theobromae. Eine neue Pilzkrankheit schädigt an Rosen unter Glas. Gärtnerbörse Gartenwelt 12:589–591

    Google Scholar 

  • Collander R (1954) The permeability of Nitella cells to non-electrolytes. Physiol Plant 7:420–445

    Article  CAS  Google Scholar 

  • Daeter W, Hartung W (1990) Compartmentation and transport of abscisic acid in mesophyll cells of intact leaves of Valerianella locusta. J Plant Physiol 136:306–312

    CAS  Google Scholar 

  • Dathe W, Rönsch H, Preiss A, Schade W, Sembdner G, Schreiber K (1981) Endogenous plant hormones of the broad bean, Vicia faba L. (-)-Jasmonic acid, a plant growth inhibitor in pericarp. Planta 153:530–535

    Article  CAS  Google Scholar 

  • Dathe W, Schindler C, Schneider G, Schmidt J, Porzel A, Jensen E, Yamaguchi I (1991) Cucurbic acid and its 6,7-stereoisomers. Phytochemistry 30:1909–1914

    Article  CAS  Google Scholar 

  • Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of petunia leaf protoplasts. Dev Biol 33:130–137

    Article  PubMed  CAS  Google Scholar 

  • Gimmler H, Hartung W (1988) Low permeability of the plasma membrane of Dunaliella parva for solutes. J Plant Physiol 133:165–172

    Google Scholar 

  • Gimmler H, Heilmann B, Demmig B, Hartung W (1981) The permeability coefficients of the plasmalemma and the chloroplast envelope of spinach mesophyll cells for phytohormones. Z Naturforsch C 36:672–678

    Google Scholar 

  • Gräbner R, Schneider G, Sembdner G (1976) Gibberelline-XLIII. Mitteilung. Fraktionierung von Gibberellinen, Gibberellinkonjugaten und anderen Phytohormonen durch DEAE-Sephadex-Chromatographie. J Chromatogr 121:110–115

    Article  PubMed  Google Scholar 

  • Hartung W (1983) The site of action of abscisic acid at the guard cell plasmalemma of Valerianella locusta. Plant Cell Environm 6:427–429

    Article  CAS  Google Scholar 

  • Hartung W, Radin JW, Hendrix DL (1988) Abscisic acid movement into the apoplastic solution of water-stressed cotton leaves. Plant Physiol 86:908–913

    PubMed  CAS  Google Scholar 

  • Hartung W, Slovik S (1991) Physicochemical properties of plant growth regulators and plant tissues determine their distribution and redistribution: Stomatal regulation by abscisic acid in leaves. New Phytol 119:361–382

    Article  CAS  Google Scholar 

  • Heilmann B, Hartung W, Gimmler H (1980) The distribution of abscisic acid between chloroplasts and cytoplasm of leaf cells and the permeability of the chloroplast envelope for abscisic acid. Z Pflanzenphysiol 97:67–78

    CAS  Google Scholar 

  • Herrmann G, Kramell H-M, Kramell R, Weidhase R, Sembdner G (1987) Biological activity of jasmonic acid conjugates. In: Schreiber K, Schütte HR, Sembdner G (eds) Conjugated plant hormones. Structure, metabolism and function. VEB Deutscher Verlag der Wissenschaften, Berlin, pp 315–322

    Google Scholar 

  • Kaiser WM, Hartung W (1981) Uptake and release of abscisic acid by isolated photoautotrophic mesophyll cells, depending upon pH gradients. Plant Physiol 68:202–206

    PubMed  CAS  Google Scholar 

  • Kaiser G, Martinoia E, Wiemken A (1982) Rapid appearance of photosynthetic products in the vacuoles isolated from barley mesophyll protoplasts by a new fast method. Z Pflanzenphysiol 107:103–113

    CAS  Google Scholar 

  • Kramell R, Schmidt J, Schneider G, Sembdner G, Schreiber K (1988) Synthesis of N-(jasmonoyl)amino acid conjugates. Tetrahedron 44:5791–5807

    Article  CAS  Google Scholar 

  • Loveys BR, Robinson SP (1987) Abscisic acid synthesis and metabolism in barley leaves and protoplasts. Plant Sci 49:23–30

    Article  CAS  Google Scholar 

  • Meyer A, Miersch O, Büttner C, Dathe W, Sembdner G (1984) Occurrence of the plant growth regulator jasmonic acid in plants. J Plant Growth Regul 3:1–8

    Article  CAS  Google Scholar 

  • Miersch O, Preiss A, Sembdner G, Schreiber K (1987a) (+)-7-iso-Jasmonic acid and related compounds from Botryodiplodia theobromae. Phytochemistry 26:1037–1039

    Article  CAS  Google Scholar 

  • Miersch O, Wrobel B, Sembdner G (1987b) Synthesis and biological activity of jasmonic acid glucosyl ester. In: Schreiber K, Schütte HR, Sembdner G (eds) Conjugated plant hormones. Structure, metabolism and function. VEB Deutscher Verlag der Wissenschaften, Berlin, pp 333–338

    Google Scholar 

  • Parthier B, Brückner C, Dathe W, Hause B, Herrmann G, Knöfel H-D, Kramell H-M, Kramell R, Lehmann J, Miersch O, Reinbothe S, Sembdner G, Wasternack C, Zur Nieden U (1992) Jasmonates: Metabolism, biological activities, and mode of action in senescence and stress responses. In: Karssen CM, van Loon LC, Vreugdenhil D (eds) Progress in plant growth regulation. Kluwer Academic Publishers, Dordrecht, pp 276–285

    Google Scholar 

  • Schmidt J, Kramell R, Brückner C, Schneider G, Sembdner G, Schreiber K, Stach J, Jensen E (1990) Gas chromatographic/mass spectrometric and tandem mass spectrometric investigations of synthetic amino acid conjugates of jasmonic acid and endogenously occurring related compounds from Vicia faba L. Biomed Environm Mass Spectrom 19:327–338

    Article  CAS  Google Scholar 

  • Schneider G, Kramell R, Brückner C (1989) Separation of diastereomeric amino acid conjugates of jasmonic acid. J Chromatogr 483:459–462

    Article  CAS  Google Scholar 

  • Sembdner G, Herrmann G, Schliemann W (1989) Growth. Progr Bot 51:134–164

    Google Scholar 

  • Sembdner G, Parthier B (1993) Biochemistry, physiological and molecular actions of jasmonates. Ann Rev Plant Physiol Plant Molec Biol 44:569–589

    Article  CAS  Google Scholar 

  • Slovik S, Baier M, Hartung W (1992) Compartmental distribution and redistribution of abscisic acid in intact leaves. I. Mathematical formulation. Planta 187:14–25

    CAS  Google Scholar 

  • Slovik S, Hartung W (1991a) Compartmental distribution and redistribution of abscisic acid in intact leaves II. Model analysis. Planta 187:26–36

    Google Scholar 

  • Slovik S, Hartung W (1992b) Compartmental distribution and redistribution of abscisic acid in intact leaves. III. Analysis of the stress-signal chain. Planta 187:37–47

    CAS  Google Scholar 

  • Ueda J, Kato J (1980) Isolation and identification of a senescence-promoting substance from wormwood (Artemisia absinthium L.). Plant Physiol 66:246–249

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. Wilfried Dathe

    Present address: Berlin-Chemie AG, Glienicker Weg 125-127, D-12489, Berlin, Germany

Authors and Affiliations

  1. Institut für Pflanzenbiochemie, Postfach 250, D-06018, Halle/Saale, Germany

    Wilfried Dathe, Heide-Mari Kramell & Robert Kramell

  2. Lehrstuhl für Botanik I, Julius-von-Sachs-Institut für Biowissenschaften der Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082, Würzburg, Germany

    Wolfgang Daeter, Stefan Slovik & Wolfram Hartung

Authors
  1. Wilfried Dathe
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  2. Heide-Mari Kramell
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  3. Wolfgang Daeter
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  4. Robert Kramell
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  5. Stefan Slovik
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  6. Wolfram Hartung
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Dathe, W., Kramell, HM., Daeter, W. et al. Uptake of jasmonic acid and related compounds by mesophyll protoplasts of the barley leaf. J Plant Growth Regul 12, 133–140 (1993). https://doi.org/10.1007/BF00189644

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  • DOI: https://doi.org/10.1007/BF00189644

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