Summary
Gamma-aminobutyric acid (GABA), the main inhibitory neurotransmitter of the central nervous system, may also be released from olivocochlear efferent nerves reaching the outer hair cells of the cochlea. In the present study the cell potential of isolated outer hair cells of the third and fourth turns of the guinea pig cochlea was measured with patch-clamp electrodes. GABA-receptor agonists and antagonists were applied extracellularly. The cell membrane hyperpolarized in a reversible manner with increasing concentrations of extracellular GABA. Half-maximal hyperpolarization (2 mV) was achieved with approximately 107− M GABA. Desensitization was not observed. The hyperpolarizing effect of GABA was potentiated by the benzodiazepine clorazepate and was blocked by picrotoxin.
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References
Altschuler RA, Fex J (1986) Efferent neurotransmitters. In: Altschuler RA, Hoffman DW, Bobbin RP (eds) Neurobiology of hearing: the cochlea. Raven Press, New York, pp 243–270
Ashmore JF (1987) A fast motile response in guinea-pig outer hair cells: the cellular basis of the cochlear amplifier. J Physiol (Lond) 388:323–347
Ashmore JF, Meech RW (1986) Ionic basis of membrane potential in outer hair cells of guinea pig cochlea. Nature 322:368–371
Bobbin RP, Fallon M, Puel J-L, Bryant G, Bledsoe SC, Zajic G, Schacht J (1990) Acetylcholine, carbachol, and GABA induce no detectable change in the length of isolated outer hair cells. Hear Res 47:39–52
Bormann J, Hamill OP, Sakmann B (1987) Mechanism of anion permeation through channels gated by glycine and λ-aminobutyric acid in mouse cultured spinal neurones. J Physiol (Lond) 385:243–286
Bradford HF (1985) Chemical neurobiology — an introduction to neurochemistry. Freemann, New York
Eybalin M, Parnaud C, Geffard M, Pujol R (1988) Immunelectron microscopy identifies several types of GABA-containing efferent synapses in the guinea-pig organ of Corti. J Neurosci 24:29–38
Fex J, Altschuler RA, Kachar B, Wenthold RJ, Zempel JM (1986) GABA visualized by immunocytochemistry in the guinea pig cochlea in axons and endings of efferent neurons. Brain Res 366:106–117
Gitter AH, Frömter E, Zenner HP (1986) Membrane potential and ion channels in isolated outer hair cells of guinea pig cochlea. ORL J Otorhinolaryngol Relat Spec 48:68–75
Gulley RL, Fex J, Wenthold RJ (1979) Uptake of putative neurotransmitters in the organ of corti. Acta Otolaryngol (Stockh) 88:177–182
Hamill OP, Bormann J, Sakmann B (1983) Activation of multiple conductance state chloride channels in spinal neurons by glycine and GABA. Nature 305:805–808
Häring P, Stähli C, Schock P, Takacs B, Möhler H (1985) Monoclonal antibodies reveal structural homogeneity of gamma-aminobutyric acid/benzodiazepine receptors in different brain areas. Proc Natl Acad Sci USA 82:4837–4841
Klinke R (1986) Neurotransmission in the innner ear. Hear Res 22:235–243
Marty A, Neher E (1983) Tight-seal whole-cell recording. In: Sakmann B, Neher E (eds) Single-channel recordings. Plenum Press, New York, pp 107–122
Plinkert PK, Möhler H, Zenner HP (1989) A subpopulation of outer hair cells possessing GABA receptors with tonotopic organization. Arch Otorhinolaryngol 246:417–422
Pritchett DB, Sontheimer H, Gorman CM, Kettenmann H, Seeburg PH, Schofield PR (1988) Transient expression shows ligand gating and allosteric potentiation of GABAA-receptor subunits. Nature 242:1306–1308
Pujol R, Lenoir M (1986) The four types of synapses in the organ of Corti. In: Altschuler RA, Bobbin RP, Hoffman DW (eds) Neurobiology of hearing: the cochlea. Raven Press, New York, pp 161–172
Schoch P, Richards JG, Häring P et al. (1985) Co-localization of GABAA receptors and benzodiazepine receptors in the brain shown by monoclonal antibodies. Nature 314:168–171
Schofield PR, Darlison MG, Fujita N et al. (1987) Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super family. Nature 328:221–227
Schwartz IR, Ryan AF (1986) Amino acid labeling patterns in the efferent innervation of the cochlea: an electron microscopic autoradiographic study. J Comp Neurol 246:500–512
Schwarz DW, Schwarz IE, Hu K (1989) Transmitter neurochemistry of the efferent neuron system innervating the labyrinth. J Otolaryngol 18:28–31
Sigel E, Barnhard EA (1983) A gamma-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex: purification and partial characterization. J Biol Chem 258: 6965–6971
Thompson GC, Cortez AM, Igarashi M (1986) GABA-like immunoreactivity in the squirrel monkey organ of Corti. Brain Res 372:72–79
Usami S, Makato I, Thompson GC (1988) Light- and electronmicroscopic study of gamma-aminobutyric-acid-like immunoreactivity in the guinea pig organ of Corti. ORL J Otorhinolaryngol Relat Spec 50:162–169
Zenner HP, Gitter AH, Zimmermann U, Schmitt U, Frömter E (1985) Die isolierte Haarzelle. Laryngol Rhinol Otol 64: 642–648
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Gitter, A.H., Zenner, H.P. γ-Aminobutyric acid receptor activation of outer hair cells in the guinea pig cochlea. Eur Arch Otorhinolaryngol 249, 62–65 (1992). https://doi.org/10.1007/BF00175674
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DOI: https://doi.org/10.1007/BF00175674