Abstract
Fibre-bound and isolated galactoglumanans from pine-wood and pine kraft pulp were hydrolysed with purified mannanases from Trichoderma reesei and Bacillus subtilis. The isolated galactoglucomannans from both wood and pulp could be hydrolysed fairly extensively with both enzymes. In addition to mixed oligomers, the fungal mannase produced mannobiose as the main hydrolysis product whereas the bacterial mannanase produced mannobiose, mannotriose and mannotetraose. Both enzymes hydrolysed the native galactoglucomannan in finely ground pinewood, whereas galactoglucomannan in pine kraft pulp was only hydrolysed by the T. ressei mannanase. Thus, mannanases exhibit different specificities on fibre-bound, modified substrates. In spite of the high enzyme loading, the degree of hydrolysis of fibre-bound substrates did not exceed 10% of the theoretical, probably due to poor accessibility of the substrates.
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References
Aurell R, Hartler N (1965) Kraft pulping of pine. Part 1. Svensk Papperstidn 68:59–68
Bailey MJ, Nevalainen KMH (1981) Induction, isolation and testing of stable Trichoderma reesei mutants with improved production of solubilizing cellulase. Enzyme Microb Technol 3:153–157
Clark TA, McDonald AG, Senior DJ, Mayers PR (1990) Mannanase and xylanase treatment of softwood chemical pulps: effects on pulp properties and bleachability. In: Kirk TK, Chang H-M (eds) Biotechnology in the Pulp and Paper Manufacture. Applications and Fundamental Investigations. Butterworth-Heinemann, Boston, pp 153–167
Genko JM, Busayasakul N (1990) Hemicellulose retention during kraft pulping. TAPPI J 73:223–233
Hansson J-Å, Hartler N (1969) Sorption of hemicelluloses on cellulose fibres. Part I. Svensk Papperstidn 72:521–530
Kantelinen A, Rättö M, Sundquist J,Ranua M, Viikari L, Linko M (1988) Hemicellulases and their potential role in bleaching. In: International Pulp Bleaching Conference. Orlando, Florida, USA, June 5–9, Tappi, Atlanta, TAPPI Proceedings pp 1–5
Kantelinen A, Rantanen T, Buchert J, Viikari L (1993) Enzymatic solubilization of fibre-bound and isolated birch xylans. J Biotechnol, in press
Luce JE (1964) Radial distribution of properties through the cell wall. Pulp and Paper Magazine of Canada 65:419–423
McCleary BV (1979a) Modes of action of β-mannanase enzymes of diverse origin on legume seed galactomannans. Phytochemistry 18:757–763
McCleary BV (1979b) Enzymatic hydrolysis, fine structureand gelling interaction of legume seed d-galacto-d-mannans. Carbohydr Res 71:205–230
McCleary BV (1988) α-d-Galactosidase from lucerne and guar seeds. Methods Enzymol 160:627–631
McCleary BV (1991) Comparison of endolytic hydrolases that depolymerize 1,4-β-d-mannan, 1,5-α-l-arabinan and 1,4-β-d-galactan. ACS Symp Ser 460:437–449
Puls J (1991) Characterization of hemicelluloses from organosolv pulping. In: Proceedings of the 6th International Symposium on Wood and Pulping Chemistry, Melbourne, April 30–May 4. pp 21–25, Melbourne, Appita
Puls J, Schorn B (1992) Acetylmannanesterase: a new component in the arsenal of wood mannan degrading enzymes. In: Kuwahara M, Shimada M (eds) Fifth International Conference on Biotechnology in the Pulp and Paper Industry. Kyoto, Japan, 27–30 May. pp 55–56
Puls J, Poutanen K, Körner H-U, Viikari L (1985) Biotechnical utilization of wood carbohydrates after steaming pretreatment. Appl Microbiol Biotechnol 22:416–423
Rättö M, Poutanen K (1988) Production of mannan degrading enzymes. Biotechnol Lett 10:661–664
Reese ET, Shibata Y (1965) β-Mannanases of fungi. Can J Microbiol 11:167–183
Simonson R (1971) The hemicellulose in the sulfate pulping process. Svensk Papperstidn 74:691–700
Sjöström E (1977) The behavior of wood polysaccharides during alkaline pulping processes. TAPPI 60:151–154
Sjöström E (1981) Wood chemistry, fundamentals and applications. Academic Press, New York
Stålbrand H, Siika-aho M, Tenkanen M, Viikari L (1993) Purification and characterization of two β-mannanases from Trichoderma reesei. J Biotechnol 29:229–242
Tenkanen M, Puls J, Rättö M, Viikari L (1993) Enzymatic deacetylation of galactoglucomannans. Appl Microb Biotechnol 39:159–165
Timell TE (1967) Recent progress in the chemistry of wood hemicelluloses. Wood Sci Technol 1:45–70
Viikari L, Kantelinen A, Rättö M, Sundquist J (1991) Enzymes in pulp and paper processing. ACS Symp Ser 460:12–22
Yllner S, Österberg K, Stockman L (1957) A study of the removal of the constituents of pine wood in the sulphate process using a continuous liquor flow method. Svensk Papperstidn 60:795–802
Zurbriggen BZ, Bailey MJ, Penttilä ME, Poutanen K, Linko M (1990) Pilot scale production of a heterologous Trichoderma reesei cellulase in Saccharomyces cerevisiae. J Biotechnol 13:267–278
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Rättö, M., Siika-aho, M., Buchert, J. et al. Enzymatic hydrolosis of isolated and fibre-bound galactoglucomannans from pine-wood and pine kraft pulp. Appl Microbiol Biotechnol 40, 449–454 (1993). https://doi.org/10.1007/BF00170409
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DOI: https://doi.org/10.1007/BF00170409