Skip to main content
SpringerLink
Log in

Overproduction of extracellular endoglucanase by genetically engineered Bacillus subtilis

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Overproduction of extracellular endoglucanase was attempted by modifying promoter region of an endoglucanase gene cloned from Bacillus subtilis BSE616 and expressing in B. subtilis DB104. A strong promoter was cloned from B. subtilis 168 chromosomal DNA and fused to the endoglucanase gene after removing its native promoter. An effective Shine-Dalgarno sequence was inserted between the promoter and the endoglucanase structural gene. The modified gene was expressed well in B. subtilis and produced 265 units of endoglucanase per mg protein that is 60 % of total protein which was secreted into culture medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Béguin, P. (1983). Anal. Biochem. 131, 333–336.

    Google Scholar 

  • Boyer, H. W., and Roulland-Dussoix, D. (1969). J. Mol. Biol. 41, 459–472.

    Google Scholar 

  • Bradford, M. M. (1976). Anal. Biochem. 72, 248–254.

    Google Scholar 

  • Kawamura, F., and Doi, R. H. (1984). J. Bacteriol. 160, 442–444.

    Google Scholar 

  • Lee, D. S., Lee, B. R., and Pack, M. Y. (1988). Biotechnol. Lett. 10, 843–848.

    Google Scholar 

  • Maniatis, T., Fritsch, E. F., and Sambrook, J. (1989). Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  • McLaughlin, J. R., Murray C. L., and Rabinowitz, J. C. (1981). J. Biol. Chem. 256, 11283–11291.

    Google Scholar 

  • Miller, G. L. (1959). Anal. Chem. 31, 426–428.

    Google Scholar 

  • Park, S. H., and Pack, M. Y. (1986). Enzyme Microbiol. Technol. 11, 313–316.

    Google Scholar 

  • Peschke, U., Beuck, V., Gujard, H., Gentz, R., and Grice, S. L. (1985). J. Mol. Biol. 186, 547–555.

    Google Scholar 

  • Shaw, W. N. (1975). Methods in Enzymology 43, 737–755.

    Google Scholar 

  • Spizizen, J. (1958). Proc. Natl. Acad. Sci. USA 44, 1072–1078.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Korea Advanced Institute of Science and Technology, 305-701, Yusung-Gu, Taejon, Korea

    Jeong Hyun Kim & M. Y. Pack

Authors
  1. Jeong Hyun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Y. Pack
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, J.H., Pack, M.Y. Overproduction of extracellular endoglucanase by genetically engineered Bacillus subtilis . Biotechnol Lett 15, 133–138 (1993). https://doi.org/10.1007/BF00133012

Download citation

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00133012

Keywords

Search

Navigation