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Simultaneous expression of genes encoding endoglucanase and β-glucosidase in Zymomonas mobilis

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Summary

As a step towards constructing strains of Z. mobilis capable of converting cellulose to ethanol, DNA fragments encoding endoglucanase (from Xanthomonas albilineans) and β-glucosidase (from either X.albilineans or Pseudomonas sp.) were linked on the same vector and transferred to Z. mobilis. All clones expressed endoglucanase. β-Glucosidase was only produced by clones containing the Xanthomonas gene, and when two copies of this gene were present the β-glucosidase activity was higher.

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Authors and Affiliations

  1. Department of Biotechnology, University of New South Wales, PO Box 1, 2033, Kensington, NSW, Australia

    Su P., Liu C. -Q, Lucas R. J., Delaney S. F. & Dunn N. W.

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  1. Su P.
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  2. Liu C. -Q
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  3. Lucas R. J.
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  4. Delaney S. F.
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  5. Dunn N. W.
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Su, P., Liu, C.Q., Lucas, R.J. et al. Simultaneous expression of genes encoding endoglucanase and β-glucosidase in Zymomonas mobilis . Biotechnol Lett 15, 979–984 (1993). https://doi.org/10.1007/BF00131768

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  • DOI: https://doi.org/10.1007/BF00131768

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