Abstract
A two-stage in vitro technique was established for the development of interspecific hybrid embryos in the genus Lens. The culture of 14-day-old fertilized ovules on MS medium supplemented with zeatin, followed by the release of the embryos from the ovular integuments, allowed the development of viable and vigorous plants.
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References
Ladizinsky G 1979. The genetics of several morphological traits in the lentil. J. Hered., 70:135–137.
Ladizinsky G, Braun D, Muehlbauer FJ The biological species of the genus Lens. Bot. Gaz. (in press).
Murashige T. and F.Skoog 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol., 15:473–497.
Raghaven V 1980. Embryo culture. in: Inter. Rev. Cytol. Suppl. 11B. I. Vasil (ed). Acad. Press, pp. 209–240.
Stewart J and Hsu CL 1977. In ovule embryo culture and seedling development in cotton (Gossypium hirsutum L.). Planta 137:113–117.
Stewart J and Hsu CL 1978. Hybridization of diploid and tetraploid cotton through in ovulo embryo culture. J. Hered. 69:404–408.
Williams E 1978. A hybrid between Trifolinum repens and T. ambiguum obtained with the aid of embryo culture. New Zeal. J. Bot., 16:499–506.
Williams E 1980. Hybrids between Trifolium ambiguum and T. hybridum obtained with the aid of embryo culture. New Zeal. J. Bot., 18:215–218.
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Cohen, D., Ladizinsky, G., Ziv, M. et al. Rescue of interspecific lens hybrids by means of embryo culture. Plant Cell Tiss Organ Cult 3, 343–347 (1984). https://doi.org/10.1007/BF00043086
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DOI: https://doi.org/10.1007/BF00043086