Abstract
Callus and cell suspension cultures of Coscinium fenestratum were established from sterile petiole segments on Murashige & Skoog (MS) medium, supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) and benzyl amino purine (BAP). The cells in the culture produced berberine as the major compound. NAA stimulated the product synthesis over 2,4-D. Presence of light inhibited the growth and enhanced the berberine synthesis.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- HPLC:
-
high pressure liquid chromatography
- IAA:
-
indole-3-acetic acid
- NAA:
-
α-naphthaleneacetic acid
- TLC:
-
thin layer chromatography
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Jayakumaran Nair, A., Sudhakaran, P.R., Madhusudana Rao, J. et al. Berberine synthesis by callus and cell suspension cultures of Coscinium fenestratum . Plant Cell Tiss Organ Cult 29, 7–10 (1992). https://doi.org/10.1007/BF00036139
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DOI: https://doi.org/10.1007/BF00036139