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Thyroid hormones in brown trout (Salmo trutta) reproduction and early development

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Abstract

Gravid brown trout (Salmo trutta) females were injected with various doses of a synthetic gonadotropin-releasing hormone analog (GnRHa), given with or without an injection of triiodothyronine (T3), in order to investigate the potential of T3 (a) to enhance the stimulatory effect of GnRHa on ovulation, and (b) to enhance the growth and survival of the produced progeny. From the time the hormonal treatments were initiated until ovulation was detected 5–38 days later, endogenous plasma T3 levels increased from an average of 3.6 to 11.6 ng ml−1. Injection with 20 mg T3 kg−1 body weight, further elevated plasma T3 levels at ovulation (16.0 ng ml−1. Mean time to ovulation was reduced significantly in fish injected with 10 μg kg−1 of GnRHa, whereas treatment with lower doses was ineffective. Injection with T3 did not enhance the ovulatory response of brown trout to GnRHa. Unfertilized eggs obtained from T3-injected females had a higher T3 content, suggesting a transfer of T3 from the maternal circulation into the oocytes. Maternal T3 injection had no effect on egg fertilization rates, embryo survival to eyeing and hatching, or the prevalence of abnormal larvae at the time of hatching. Length and weight gain of the progeny during yolk absorption was also not influenced by maternal T3 treatment. At the completion of yolk-sac absorption, progeny from females injected with T3 had a higher prevalence of skeletal abnormalities than controls. The results suggest that in teleosts like brown trout, which have high endogenous circulating T3 levels, treatment of females with T3 does not enhance responsiveness to GnRHa and it has the potential for deleterious effects on their offspring.

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  1. Costadinos C. Mylonas

    Present address: Center of Marine Biotechnology, University of Maryland System, 600 E. Lombard St., Baltimore, Maryland, 21202, U.S.A.

Authors and Affiliations

  1. Department of Zoology, North Carolina State University, Raleigh, N.C., 27695, U.S.A.

    Costadinos C. Mylonas, Craig V. Sullivan & Jeffrey M. Hinshaw

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  1. Costadinos C. Mylonas
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  2. Craig V. Sullivan
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Correspondence to Costadinos C. Mylonas.

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Mylonas, C.C., Sullivan, C.V. & Hinshaw, J.M. Thyroid hormones in brown trout (Salmo trutta) reproduction and early development. Fish Physiol Biochem 13, 485–493 (1994). https://doi.org/10.1007/BF00004331

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