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Histological and ultrastructural evidence for the role of gonadal steroid hormones in sex change in the protogynous wrasse Thalassoma duperrey

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The process of sex change in the protogynous wrasse, Thalassoma duperrey, was investigated through histological and ultrastructural observations on the gonads of females changing sex to male. Changes in plasma steroid levels concomitant with structural changes were measured by radioimmunoassay. The process of sex change from ovary to testis was divided into six stages on the basis of changes in the structure of the germinal and somatic elements. Ovaries of females were filled with vitellogenic oocytes during the breeding season, but contained no spermatogenic tissue (Stage 1). At the commencement of sex change (Stage 2), vitellogenic oocytes began to degenerate, and were ingested by macrophagous cells. This stage was accompanied by a rapid drop in plasma levels of estradiol-17β. Thereafter, previtellogenic oocytes (Stage 3) also began to degenerate, and aggregations of stromal tissue, and loose connective tissue were observed in the central region of the lamellae. Steroid producing cells (Leydig cells), developed at the border of this loose connective tissue. Presumed spermatogonia proliferated on the periphery of the lamellae, and Leydig cells increased in size and number (Stage 4). Spermatogonia formed cysts, and underwent spermatogenesis (Stage 5). Finally, sex change to male was considered complete, with the beginning of active spermatogenesis and spermiation (Stage 6). Plasma levels of testosterone remained low throughout the sex change, but a second androgen, 11-ketotestosterone increased gradually in parallel to the increased numbers of Leydig cells and spermatogonia. Preliminary in vitro incubation of gonads with salmon gonadotropin, revealed that sex-changed males had higher levels of 11-ketotestosterone production than did females, while females had higher levels of estradiol-17β production than did males. Production of both these steroids increased in a dose-related fashion with increasing doses of gonadotropin.

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Authors and Affiliations

  1. Department of Zoology, Faculty of Medicine, Teikyo University, Hachioji Tokyo, 192-03, Japan

    Masaru Nakamura

  2. Department of Zoology and Hawaii Institute of Marine Biology, University of Hawaii, Honolulu, Hawaii, 96822, U.S.A.

    Thomas F. Hourigan & E. Gordon Grau

  3. Laboratory of Fresh-water Fish Culture, Faculty of Fisheries, Hokkaido University, Hakodate, 041, Japan

    Kohei Yamauchi

  4. Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, 444, Japan

    Yoshitaka Nagahama

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  1. Masaru Nakamura
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Nakamura, M., Hourigan, T.F., Yamauchi, K. et al. Histological and ultrastructural evidence for the role of gonadal steroid hormones in sex change in the protogynous wrasse Thalassoma duperrey . Environ Biol Fish 24, 117–136 (1989). https://doi.org/10.1007/BF00001282

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