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TRIM37 promoted the growth and migration of the pancreatic cancer cells

  • Original Article
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Tumor Biology

Abstract

Increasing evidence indicated that tripartite motif containing 37 (TRIM37) was involved in the tumorigenesis of several cancer types. However, its expression pattern and biological functions in pancreatic ductal adenocarcinoma (PDAC) remained unknown. In this study, real-time PCR, Western blot and immunohistochemistry was performed to examine the expression of TRIM37 in the pancreatic cancerous tissues. Colony formation assay and cell migration assay were performed to study the functions of TRIM37 in pancreatic cancer cells. Dual-luciferase assay was performed to study the regulation of TRIM37 on beta-catenin/TCF signaling. It was found that the expression level of TRIM37 was significantly higher in pancreatic cancerous tissues compared with the adjacent normal tissues. Function analysis indicated that overexpression of TRIM37 promoted the growth and migration of the pancreatic cancer cells, while knocking down the expression of TRIM37 inhibited the growth and migration of the pancreatic cancer cells. The molecular mechanism study suggested that TRIM37 interacted with beta-catenin and activated the transcriptional activity of beta-catenin/TCF complex as well as the expression of its downstream target genes. Taken together, our study showed the oncogenic roles of TRIM37 in pancreatic cancer, and TRIM37 might be a promising target for pancreatic cancer treatment.

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References

  1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin. 2015;65:5–29.

    Article  PubMed  Google Scholar 

  2. Liang C, Wang Z, Li YY, Yu BH, Zhang F, Li HY. Mir-33a suppresses the nuclear translocation of beta-catenin to enhance gemcitabine sensitivity in human pancreatic cancer cells. Tumour Biol. (ahead of print). 2015.

  3. Jiang S, Zhu L, Tang H, et al. Ape1 regulates wnt/beta-catenin signaling through its redox functional domain in pancreatic cancer cells. Int J Oncol. (ahead of print). 2015.

  4. Chen X, Liu X, Lang H, Zhang S, Luo Y, Zhang J. S100 calcium-binding protein a6 promotes epithelial-mesenchymal transition through beta-catenin in pancreatic cancer cell line. PLoS One. 2015;10:e0121319.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Kumaran S, Samantha KS, Halagowder D. Does beta-catenin cross-regulate NFkappaB signalling in pancreatic cancer and chronic pancreatitis? Pathobiology. 2015;82:28–35.

    Article  CAS  PubMed  Google Scholar 

  6. Xu W, Wang Z, Zhang W, et al. Mutated k-ras activates cdk8 to stimulate the epithelial-to-mesenchymal transition in pancreatic cancer in part via the wnt/beta-catenin signaling pathway. Cancer Lett. 2015;356:613–27.

    Article  CAS  PubMed  Google Scholar 

  7. Macdonald BT, Semenov MV, He X. Snapshot: Wnt/beta-catenin signaling. Cell. 2007;131:1204.

    Article  CAS  PubMed  Google Scholar 

  8. Clevers H, Nusse R. Wnt/beta-catenin signaling and disease. Cell. 2012;149:1192–205.

    Article  CAS  PubMed  Google Scholar 

  9. Clevers H. Wnt/beta-catenin signaling in development and disease. Cell. 2006;127:469–80.

    Article  CAS  PubMed  Google Scholar 

  10. Kallijarvi J, Lahtinen U, Hamalainen R, Lipsanen-Nyman M, Palvimo JJ, Lehesjoki AE. Trim37 defective in mulibrey nanism is a novel ring finger ubiquitin e3 ligase. Exp Cell Res. 2005;308:146–55.

    Article  PubMed  Google Scholar 

  11. Bhatnagar S, Gazin C, Chamberlain L, et al. Trim37 is a new histone h2a ubiquitin ligase and breast cancer oncoprotein. Nature. 2015;516:116–20.

    Google Scholar 

  12. Dimova I, Orsetti B, Negre V, et al. Genomic markers for ovarian cancer at chromosomes 1, 8 and 17 revealed by array cgh analysis. Tumori. 2009;95:357–66.

    CAS  PubMed  Google Scholar 

  13. Karlberg S, Lipsanen-Nyman M, Lassus H, Kallijarvi J, Lehesjoki AE, Butzow R. Gynecological tumors in mulibrey nanism and role for ring finger protein trim37 in the pathogenesis of ovarian fibrothecomas. Mod Pathol. 2009;22:570–8.

    Article  CAS  PubMed  Google Scholar 

  14. Brodtkorb M, Lingjaerde OC, Huse K, Troen G, et al. Whole-genome integrative analysis reveals expression signatures predicting transformation in follicular lymphoma. Blood. 2014;123:1051–4.

    Article  CAS  PubMed  Google Scholar 

  15. Balestra FR, Strnad P, Fluckiger I, Gonczy P. Discovering regulators of centriole biogenesis through siRNA-based functional genomics in human cells. Dev Cell. 2013;25:555–71.

    Article  CAS  PubMed  Google Scholar 

  16. Tuna M, Smid M, Martens JW, Foekens JA. Prognostic value of acquired uniparental disomy (aupd) in primary breast cancer. Breast Cancer Res Treat. 2012;132:189–96.

    Article  CAS  PubMed  Google Scholar 

  17. Hamalainen RH, Mowat D, Gabbett MT, O’Brien TA, Kallijarvi J, Lehesjoki AE. Wilms’ tumor and novel trim37 mutations in an Australian patient with mulibrey nanism. Clin Genet. 2006;70:473–9.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biliary-Hepatic Surgery, Affiliated Hospital of Guiyang Medical College, 28th Guiyi Road, Guiyang, Guizhou, 550001, China

    Jianxin Jiang, She Tian, Chao Yu, Meiyuan Chen & Chengyi Sun

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  1. Jianxin Jiang
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  2. She Tian
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  3. Chao Yu
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  4. Meiyuan Chen
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  5. Chengyi Sun
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Correspondence to Chengyi Sun.

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Figure S1

The expression of TRIM37 did not affect the expression of Wnt 3a. BXPC3 and Suit2 cells were overexpressed TRIM37 or knocked down the expression of TRIM37, and the mRNA level of Wnt3a was examined using qPCR. (JPEG 837 kb)

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Jiang, J., Tian, S., Yu, C. et al. TRIM37 promoted the growth and migration of the pancreatic cancer cells. Tumor Biol. 37, 2629–2634 (2016). https://doi.org/10.1007/s13277-015-4078-7

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  • DOI: https://doi.org/10.1007/s13277-015-4078-7

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