Abstract
The increased consumption of meat products, such as hamburger in large cities such as Tehran, has highlighted the importance of quality control for these products. Due to the escalating cost of red meat, and the difficulty of detecting adulteration in ground meat, the replacement of red meat with cheaper animal and plant proteins in these products is clearly possible. As a result, the aim of this study was to investigate the validity of labeling in premium hamburgers made of beef. In addition, the presence of soybean and chicken meat, which constitutes commercial fraud in premium hamburgers, was detected using a sensitive and quick multiplex-PCR method. In total, 10 specified brands of premium hamburgers purported to consist of beef were collected from markets in Tehran City, Iran. DNA was extracted from the premium hamburgers, then, simplex-PCR and multiplex-PCR fwere optimized using specific beef, chicken and soybean primers. The 118, 183, and 274 bp fragments, were amplified in all samples from soybean lectin, 12 s rRNA, and mitochondrial cytochrome b genes, respectively. The results indicated the addition of chicken meat and soybean in the premium hamburgers which were not indicated on their labels.
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References
Abd El-Nasser M, Labieb HY, Abd El-Aziz DM (2010) Detection of native and modified soybean in some meat products in Assiut city, Egypt. Ass Univ Bull Environ Res 13(1):27–35
Amaral JS, Santos CG, Melo VS, Oliveira MBPP, Mafra I (2013) Authentication of a traditional game meat sausage (Alheira) by species-specific PCR assays to detect hare, rabbit, red deer, pork and cow meats. Food Res Int. doi:10.1016/j.foodres.2013.11.003
Burns M, Shanahan D, Valdivia H, Harris N (2003) Quantitative event-specific multiplex PCR detection of Roundup Ready soya using LabChip technology. Eur Food Res Technol 216(5):428–33
Cammà C, Domenico M, Monaco F (2012) Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures. Food Control 23:400–404
Cheng X, He W, Huang M, Zhou G (2014) Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds. Food Res Int. doi:10.1016/j.foodres.2014.01.047
Chun Chi L, Lai Ling F, Po Kwok C, Cheuk Mana L, Kwok Fai C, Shuk Han C (2014) A rapid low-cost high-density DNA-based multi-detection test for routine inspection of meat species. Meat Sci 96:922–929
Dalmasso A, Fontanella E, Piatti P, Civera T, Rosati S, Bottero MT (2004) A multiplex PCR assay for the identification of animal species in feedstuffs. Mol Cell Probes 18(2):81–87
Doosti A, Ghasemi Dehkordi P, Rahimi E (2011) Molecular assay to fraud identification of meat products. Food Sci Technol 51(1):148–152
Flores-Munguia ME, Bermudez-Almada MC, Vazquez-Moreno L (2000) A research note: Detection of adulteration in processed traditional meat products. Muscle Foods 11:319–325
Forte VT, Pinto A, Martino C, Tantillo GM, Grasso G, Schena FP (2005) A general multiplex-PCR assay for the general detection of genetically modified soybeana and maize. Food Control 16:535–539
Ghovvati S, Nassiri MR, Mirhoseini SZ, Heravi Moussavi A, Javadmanesh A (2009) Fraud identification in industrial meat products by multiplex PCR assay. Food Control 20:696–699
Gomez Galan A, Brohee M, Silva E, Henge JVA, Chassaigne H (2011) Development of a real-time PCR method for the simultaneous detection of soybeana and lupin mitochondrial DNA as markers for the presence of allergens in processed food. Food Chem 127:834–841
Greiner R, Konietzny U, Villavicencio A (2005) Qualitative and quantitative detection of genetically modified maize and soybean in processed foods sold commercially in Brazil by PCR-based methods. Food Control 16:753–759
Hiu YH (2007) Appendix Standards for Meat, Poultry and Seafood in the United States. In: Nollet LML (ed) Meat, Poultry and Seafood Quality. Wiley, Ames, pp. 589–694
Hosseini H, Barazandegan K, Akhondzadeh A, Shemshadi B, Tavakoli HR, Khaksar R (2009) Determination the kind of meat content of Patties marketed in Tehran in 2009. J Food Sci Technol 6:95–100[in Persian]
Ilhak OI, Arslan A (2007) Identification of meat species by polymerase chain reaction (PCR) Technique. Turk J Vet Anim Sci 31:159–163
Jahed Khaniki G, Rokni N (2004) Histological detection of soybeana in freezing raw hamburger of Iran. Pajouhesh and Sazandegi 62:71–75[in Persian]
Karabasanavar SN, Singh SP, Kumar D, Shebannavar S (2014) Detection of pork adulteration by highly-specific PCR assay of mitochondrial D-loop. Food Chem 145:530–534
Kesmen Z, Celebi Y, Güllüce A, Yetim H (2013) Detection of seagull meat in meat mixtures using real-time PCR analysis. Food Control 34:47–49
Kim JH, Jeong D, Kim YR, Kwon YK, Rhee GS, Zhang D, Kim HY (2013) Development of a multiplex PCR method for testing six GM soybeanbean events. Food Control 31:366–371
Kitpipit T, Sittichan K, Thanakiatkrai P (2013) Are these food products fraudulent? Rapid and novel triplex-direct PCR assay for meat identification. Forensic Sci Int: Genet Suppl Ser 4:e33–e34
Mafra I, Ferreira IMPLVO, Oliveira MBPP (2008) Food authentication by PCR-based methods. Eur Food Res Technol 227:649–665. doi:10.1007/s00217-007-0782-x
Mane BG, Mendiratta SK, Tiwari AK (2009) Polymerase chain reaction assay for identification of chicken in meat and meat products. Food Chem 116:806–810
Matsunaga T, Chikuni K, Tanabeb R, Muroyab S, Shibata K, Yamadaa J, Shinmura Y (1999) A quick and simple method for the identication of meat species and meat products by PCR assay. Meat Sci 51:143–148
Meyer R, Chardonnens F, Hiibner P, Liithy J (1996) Polymerase chain reaction (PCR) in the quality and safety assurance of food: Detection of soya in processed meat products. Z Lebensm Unterr For 203:339–344
Morrissey PA, Olbrantz K, Greaser ML (1982) A simple, sensitive enzymatic method for quantitation of soybeana proteins in soybeana-meat blends. Meat Sci 7:109–116
Murugaiah C, Noor Z, Mastakim M, Maurice Bilung L, Selamat J, Radu S (2009) Meat species identification and Halal authentication analysis using mitochondrial DNA. Meat Sci 83:57–61
Opara LU (2003) Traceability in agriculture and food supply chain: a review of basic concepts, technological implications, and future prospects. J Food Agric Environ 1:101–106
Pascoal A, Prado M, Castro J, Cepeda A, Barros J (2004) Survey of authenticity of meat species in food products subjected to different technological processes, by means of PCR-RFLP. Anal Eur Food Res technol 218:306–312
Renčová E, Tremlová B (2009) ELISA for detection of soybeana proteins in meat products. Acta Vet Brno 78:667–671
Rokni N, Rezaian M, Noori N, Ebrahimpoor F (2004) Detection of unauthorized tissue in some of cured meat products of Tehran with histological method. Pajouhesh and Sazandegi 65:2–5[in Persian]
Safdar M, Abasıyanık MF (2013) Development of fast multiplex real-time PCR assays based on EvaGreen fluorescence dye for identification of beef and soybeanbean origins in processed sausages. Food Res Int 54:1652–1656
Sakaridis I, Ganopoulos I, Argiriou A, Tsaftaris A (2013) A fast and accurate method for controlling the correct labeling of products containing buffalo meat using High Resolution Melting (HRM) analysis. Meat Sci 94:84–88
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. Cold Spring Harbor Laboratory Press, New York
Soares S, Amaral JS, Beatriz PP, Oliveira M, Mafra I (2012) A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products. Meat Sci 94:115–120. doi:10.1016/j.meatsci.2012.12.012
Soares S, Mafra I, Amaral SJ, Olivei MBPP (2010) A PCR assay to detect trace amounts of soybeanbean in meat sausages. Int J Food Sci Technol 45:2581–2588. doi:10.1111/j.1365-2621.2010.02421.x
Stamoulis P, Stamatis C, Sarafidou T, Mamuris Z (2010) Development and application of molecular markers for poultry meat identification in food chain. Food Control 21:1061–1065
Taski-Ajdukovic K, Nikolic Z, Vujakovic M, Milosevic M, Ignjatov M, Petrovic D (2009) Detection of genetically modified organisms in processed meat products on the Serbian food market. Meat Sci 81:230–232
Xu X, Li Y, Zhao H, Wen SY, Wang SQ, Huang J, Huang KL, Luo YB (2005) Rapid and reliable detection and identification of GM events using multiplex PCR coupled with oligonucleotide microarray. J Agric Food Chem 53(10):3789–3794
Zilio Dinon A, Treml D, Souza de Mello D, Carolina Maisonnave Arisi A (2010) Monitoring of GMO in Brazilian processed meat and soybean-based products from 2007 to 2008. Food Compos Anal 23:226–229
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Tafvizi, F., Hashemzadegan, M. Specific identification of chicken and soybean fraud in premium burgers using multiplex-PCR method. J Food Sci Technol 53, 816–823 (2016). https://doi.org/10.1007/s13197-015-1771-x
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DOI: https://doi.org/10.1007/s13197-015-1771-x