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Primers for the amplification of the MHC IIβ chain exon 2 in the Atlantic goliath grouper (Epinephelus itajara)

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Abstract

In the present study we designed a pair of primers to amplify the exon 2 of the MHC IIβ chain of the Atlantic goliath grouper, which is responsible for the recognition of pathogenic molecules and the regulation of the immune system. Future analyses of this region may provide an important database to understand the evolutionary processes affecting the populations of the goliath grouper, and to predict the conservation perspectives in the species.

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References

  • Aguilar-Perera A, González-Salas C, Tuz-Sulub A, Villegas-Hernández H (2009) Fishery of the goliath grouper, Epinephelus itajara (Teleostei: Epinephelidae) based on local ecological knowledge and fishery records in Yucatan, Mexico. Rev Biol Trop 57:557–566

    PubMed  Google Scholar 

  • Eizaguirre C, Lenz TL (2010) Major histocompatability complex polymorphism: dynamics and consequences of parasite-mediated local adaptation in fishes. J Fish Biol 77:2023–2047

    Article  CAS  PubMed  Google Scholar 

  • Ellis SA, Bontrop RE, Antczak DF et al (2006) ISAG/IUIS-VIC comparative MHC nomenclature committee report, 2005. Immunogenetics 57:953–958

    Article  PubMed  Google Scholar 

  • Frias-Torres S (2006) Habitat use of juvenile goliath grouper Epinephelus itajara in the Florida Keys, USA. Endang Species Res 2:1–6

    Article  Google Scholar 

  • Gerhardinger LC, Marenzi RC, Bertoncini AA, Medeiros RP, Hostim-Silva M (2006) Local Ecological Knowledge on the goliath grouper Epinephelus itajara (Teleostei: Serranidae) in Southern Brazil. Neotrop Ichthyol 4:441–450

    Article  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

  • He Y, Xi D, Leng J et al (2014) Genetic variability of MHC class II DQB exon 2 alleles in yak (Bos grunniens). Mol Biol Rep. doi:10.1007/s11033-014-3071-3

    PubMed Central  Google Scholar 

  • Koenig CC, Coleman FC, Eklund AM, Schull J, Ueland J (2007) Mangroves as essential nursery habitat for goliath grouper (Epinephelus itajara). Bull Mar Sci 80:567–586

    Google Scholar 

  • Mann DA, Locascio JV, Coleman FC, Koenig CC (2009) Goliath grouper Epinephelus itajara sound production and movement patterns on aggregation sites. Endang Species Res 7:229–236

    Article  Google Scholar 

  • May S, Beebee TJC (2009) Characterization of major histocompatibility complex class II alleles in the natterjack toad, Bufo calamita. Conserv Genet Res 1:415–417

    Google Scholar 

  • Sadovy de Mitcheson Y, Craig MT, Bertoncini AA et al (2012) Fishing groupers towards extinction: a global assessment of threats and extinction risks in a billion dollar fishery. Fish Fish. doi:10.1111/j1467-2979201100455x

    Google Scholar 

  • Sadovy Y, Eklund AM (1999) Synopsis of biological data on the Nassau Grouper, Ephinephelus striatus (Bloch, 1792), and the Jewfish, E. itajara (Linchtenstein, 1822) NOAA Technical Report NMFS 146:1–68

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Could Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Silva-Oliveira GC, Rêgo PS, Schneider H, Sampaio I, Vallinoto M (2008) Genetic characterization of populations of the critically endangered goliath grouper (Epinephelus itajara, Serranidae) from the Northern Brazilian coast. Genet Mol Biol 31:988–994

    Article  CAS  Google Scholar 

  • Silva-Oliveira GC, Silva ABC, Oliveira Y, Nunes ZP, Torres RA, Sampaio I, Vallinoto M (2013) New nuclear primers for molecular studies of Epinephelidae fishes. Conserv Genet Resour 5:165–168

    Google Scholar 

  • Sonsthagen SA, Fales K, Jay CV, Sage GK, Talbot SL (2014) Spatial variation and low diversity in the major histocompatibility complex in walrus (Odobenus rosmarus). Polar Biol. doi:10.1007/s00300-014-1450-9

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tobin A, Currey L, Simpfendorfer C (2013) Informing the vulnerability of species to spawning aggregation fishing using commercial catch data. Fish Res 143:47–56

    Article  Google Scholar 

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Acknowledgments

We are grateful to ICMBio and to the Meros do Brasil team for providing the samples of E. itajara, especially the Dr. Beatrice Padovani Ferreira. This study was supported by CNPq and FAPESPA (Grant 306233/2009-6 to IS, and PRONEX 2007, respectively), and by CAPES and CNPq stipend. The authors are also grateful to CNPq for the researcher fellowships provided to RAT, IS and MV.

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Authors and Affiliations

  1. Universidade Federal do Pará, Campus de Bragança, Alameda Leandro Ribeiro s/n, Bragança, Pará, 68600-000, Brazil

    Gláucia C. Silva-Oliveira, Anete B. C. Silva, Zélia Nunes, Iracilda Sampaio & Marcelo Vallinoto

  2. Institut Français de Recherche Pour L’exploitation de la Mer, IFREMER, Issy-les-Moulineaux, France

    Fabian Blanchard

  3. Laboratório de Genômica Evolutiva and Ambiental, Departamento de Zoologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brazil

    Rodrigo A. Torres

  4. CIBIO/UP, Centro de Investigação em Biodiversidade and Recursos Genéticos da Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal

    Marcelo Vallinoto

Authors
  1. Gláucia C. Silva-Oliveira
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  2. Anete B. C. Silva
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  3. Fabian Blanchard
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  4. Zélia Nunes
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  5. Rodrigo A. Torres
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  6. Iracilda Sampaio
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  7. Marcelo Vallinoto
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Corresponding author

Correspondence to Marcelo Vallinoto.

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Silva-Oliveira, G.C., Silva, A.B.C., Blanchard, F. et al. Primers for the amplification of the MHC IIβ chain exon 2 in the Atlantic goliath grouper (Epinephelus itajara). Conservation Genet Resour 6, 523–525 (2014). https://doi.org/10.1007/s12686-014-0167-y

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  • DOI: https://doi.org/10.1007/s12686-014-0167-y

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