Abstract
A multiplex PCR assay was developed for the identification of polymorphic microsatellite loci (8) for a North African toad, Amietophrynus mauritanicus, using 454 GS-FLX Titanium pyrosequencing. The utility of these markers was evaluated in 40 individuals from two different Moroccan populations. Number of alleles per locus ranged from 3 to 15, with expected and observed heterozygosities ranging from 0.226 to 0.937 and 0.172 to 0.950 respectively. None departures from Hardy–Weinberg equilibrium were observed at all loci. These markers will be valuable tools for population genetic studies in conservation management for this species.
This is a preview of subscription content, log in via an institution to check access.
References
Emel SL, Storfer A (2012) A decade of amphibian population genetic studies: synthesis and recommendations. Conserv Genet 13:1685–1689
Guichoux E, Lagache L, Wagner S, Chaumeil P, Léger P, Lepais O, Lepoittevin C, Malausa T, Revardel E, Salin F, Petit RJ (2011) Current trends in microsatellite genotyping. Mol Ecol Resour 11:591–611
IUCN, Conservation International, NatureServe (2011) An analysis of amphibians on the 2008 IUCN Red List. http://www.icunredlist.org. Accessed 27 Jan 2012
Jombart T (2008) adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24:1403–1405
Malausa T, Gilles A, Meglecz E, Blanquart H, Duthoy S, Costedoat C, Dubut V, Pech N, Castagnone-Sereno P, Delye C, Feau N, Frey P, Gauthier P, Guillemaud T, Hazard L, Le Corre V, Lung-Escarmant B, Male PJ, Ferreira S, Martin JF (2011) High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Mol Ecol Resour 11:638–644
Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26(3):403–404
Morin PA, Luikart G, Wayne RK, The SNP Workshop Group (2004) SNPs in ecology, evolution and conservation. Trends Ecol Evol 19:208–216
Ouberg NJ, Piquot Y, van Groenendael JM (1999) Population genetics, molecular markers and the study of dispersal in plants. J Ecol 87:551–568
Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106
Schleich HH, Kastle W, Kabisch K (1996) Amphibians and reptiles of North Africa. Koeltz Scientific Books, Germany, p 630
Vignal A, Milan D, SanCristobal M, Eggen A (2002) A review on SNP and other types of molecular markers and their use in animal genetics. Genet Sel Evol 34:275
Acknowledgments
This study was supported by the Service de Systématique Moléculaire of the Muséum National d’Histoire Naturelle (CNRS-UMS 2700) and ANR 6ème extinction ANR-09-PEXT-004, MOHMIE, ‘MOdern Human installation in Morocco, Influence on the small terrestrial vertebrate biodiversity and Evolution’ (C.Denys). We would like to thank Annemarie Ohler for collecting specimens for tissue samples.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lalis, A., Munoz, C. & Lambourdière, J. Development and characterization of 8 polymorphic microsatellite loci in a North African toad, Amietophrynus mauritanicus (Schlegel, 1841). Conservation Genet Resour 5, 1039–1041 (2013). https://doi.org/10.1007/s12686-013-9963-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-013-9963-z