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SNP discovery in a reef-forming glass sponge, Aphrocallistes vastus, using the Ion Torrent next generation sequencing platform

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Abstract

Using next generation sequencing (NGS), we located 1,121 single nucleotide polymorphism (SNP) markers in a reef-forming glass sponge, Aphrocallistes vastus. 16 SNPs were validated using Sanger sequencing. SNPs developed here are available for use in describing the genetic structure of glass sponge reefs, and demonstrate that NGS methods overcome problems posed by genomic DNA in some invertebrates.

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Acknowledgments

This work was funded by an NSERC Strategic Network grant (CHONe) and an NSERC Ship Time grant to SPL. We thank D Coltman and the Molecular Biology Service Unit (University of Alberta) for assistance and use of facilities.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2E9, Canada

    Rachel R. Brown, Corey S. Davis & Sally P. Leys

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  1. Rachel R. Brown
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  2. Corey S. Davis
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  3. Sally P. Leys
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Correspondence to Rachel R. Brown.

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Brown, R.R., Davis, C.S. & Leys, S.P. SNP discovery in a reef-forming glass sponge, Aphrocallistes vastus, using the Ion Torrent next generation sequencing platform. Conservation Genet Resour 6, 49–51 (2014). https://doi.org/10.1007/s12686-013-0073-8

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  • DOI: https://doi.org/10.1007/s12686-013-0073-8

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