Conservation Genetics Resources

, Volume 5, Issue 2, pp 327–330

Molecular forensics in the precious Mediterranean red coral, Corallium rubrum: testing DNA extraction and microsatellite genotyping using dried colonies

  • Jean-Baptiste Ledoux
  • Didier Aurelle
  • Jean-Pierre Féral
  • Joaquim Garrabou
Technical Note

DOI: 10.1007/s12686-012-9795-2

Cite this article as:
Ledoux, JB., Aurelle, D., Féral, JP. et al. Conservation Genet Resour (2013) 5: 327. doi:10.1007/s12686-012-9795-2

Abstract

Corallium rubrum is an overharvested precious coral submitted to strong environmental pressures. The development of new management tools such as assignment tests is crucial to assess the origin of traded colonies and to reinforce existing regulations in order to ultimately reduce poaching. As a starting point, we test the feasibility of DNA extractions and PCR amplifications of ten microsatellites using dried tissues such as those that can be obtained from traded or seized colonies. We genotype the same ten colonies conserved in alcohol and after five drying periods (from 7 days to 8 months) as well as ten dried colonies coming from a seizure conducted in 2009. Estimating the rate of negative PCRs through time, PCR repeatability and frequencies of null alleles, we demonstrate that dried colonies can be successfully genotyped. This study opens new avenues for the conservation of C. rubrum and other precious corals.

Keywords

Poaching Corallium rubrum Dried tissues Degraded DNA DNA extraction Microsatellites Genotyping 

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Jean-Baptiste Ledoux
    • 1
    • 2
    • 3
  • Didier Aurelle
    • 3
  • Jean-Pierre Féral
    • 3
  • Joaquim Garrabou
    • 1
    • 3
  1. 1.Institut de Ciències del MarCSICBarcelonaSpain
  2. 2.CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e AmbientalUniversidade do PortoPortoPortugal
  3. 3.UMR 7263 IMBE, CNRSAix-Marseille UniversityMarseilleFrance