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Isolation of microsatellite markers from tiger grouper (Mycteroperca tigris) and characterization in yellowfin grouper (Mycteroperca venenosa), coney (Cephalopholis fulva), and red hind (Epinephelus guttatus)

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Abstract

Thirty-six microsatellite markers were isolated from an enriched genomic library of tiger grouper (Mycteroperca tigris) and characterized in 20 individuals each for yellowfin grouper (Mycteroperca venenosa), coney (Cephalopholis fulva), and red hind (Epinephelus guttatus). The microsatellites include 23 dinucleotide repeats, twelve trinucleotide repeats, and one tetranucleotide repeat. The microsatellites characterized in this study will be useful for further evaluation of population-genetic indices in the economically important subfamily Epinephelinae.

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References

  • Estoup A, Larigiader CR, Perrot E, Chourrout D (1996) Rapid one tube DNA extraction for reliable PCR detection of fish polymorphic markers and transgenes. Mol Mar Biol Biotech 5:295–298

    CAS  Google Scholar 

  • Heemstra PC, Randall JE (1993) FAO species catalogue. groupers of the world (family serranidae, subfamily epinephelinae). An annotated and illustrated catalogue of the grouper, Rockcod, Hind, coral grouper and Lyretail species to date. FAO Fish Circ 16:276–278

    Google Scholar 

  • Karlsson S, Renshaw MA, Rexroad CE, Gold JR (2008) PCR primers for 100 microsatellites in red drum (Sciaenops ocellatus). Mol Ecol Resour 8:393–398

    Article  PubMed  CAS  Google Scholar 

  • Lewis PO, Zaykin D (2001) Genetic data analysis: computer program for the analysis of allelic data. Version 1.0 (d16c). Free program distributed by the authors via the internet from http://hydrodictyon.eeb.uconn.edu/people/plewis/software.php

  • Maljkovic A, Van Leeuwen TE, Cove SN (2008) Predation on the invasive red lionfish, Pterois volitans (pisces: scorpaenidae), by native groupers in the Bahamas. Coral Reefs 27:501

    Article  Google Scholar 

  • Mumby PJ, Harborne AR, Brumbaugh DR (2011) Grouper as a natural biocontrol of invasive lionfish. PLoS One 6:e21510

    Article  PubMed  CAS  Google Scholar 

  • Planes S, Jones GP, Thorrold SR (2009) Larval dispersal connects fish populations in a network of marine protected areas. PNAS 106:5693–5697

    Article  PubMed  CAS  Google Scholar 

  • Renshaw MA, Nemeth RS, Gold JR (2011) Isolation and characterization of microsatellite markers from yellowfin grouper, Mycteroperca venenosa. Conserv Genet Resour 3:341–344

    Article  Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Robinson J, Samoilys MA, Grandcourt E, Julie D, Cedras M, Gerry C (2011) The importance of targeted spawning aggregation fishing to the management of Seychelles’ trap fishery. Fish Res 112:96–103

    Article  Google Scholar 

  • Sadovy de Mitcheson Y, Cornish A, Domeier M, Colin PL, Russell M, Lindeman KC (2008) A global baseline for spawning aggregations of reef fishes. Conserv Biol 22:1233–1244

    Article  PubMed  Google Scholar 

  • Sadovy Y, Colin PL, Domeier ML (1994) Aggregation and spawning in the tiger grouper, Mycteroperca tigris. Copeia 2:511–516

    Article  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • Ward RD (2000) Genetics in fisheries management. Hydrobiologia 420:191–201

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank E. Kadison and D. Olsen for assistance with sampling. Work was supported by the Cooperative Research Program (CRP) of the National Marine Fisheries Service, U.S. Department of Commerce (Grant NA08NMF4540400), and by Texas AgriLife Research (Project H-6703). This paper is number 87 in the series ‘Genetic Studies in Marine Fishes,’ Contribution No. 213 of the Center for Biosytematics and Biodiversity at Texas A & M University, and Contribution number 86 of the Center for Marine and Environmental Studies at University of the Virgin Islands.

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Authors and Affiliations

  1. Center for Biosystematics and Biodiversity, Texas A and M University, College Station, TX, 77843-2258, USA

    Mark A. Renshaw & John R. Gold

  2. Center for Marine and Environmental Studies, University of the Virgin Islands, 2 John Brewer’s Bay, St. Thomas, VI, 00802-9990, USA

    Richard S. Nemeth

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  1. Mark A. Renshaw
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  2. Richard S. Nemeth
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  3. John R. Gold
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Correspondence to Mark A. Renshaw.

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Renshaw, M.A., Nemeth, R.S. & Gold, J.R. Isolation of microsatellite markers from tiger grouper (Mycteroperca tigris) and characterization in yellowfin grouper (Mycteroperca venenosa), coney (Cephalopholis fulva), and red hind (Epinephelus guttatus). Conservation Genet Resour 4, 1049–1054 (2012). https://doi.org/10.1007/s12686-012-9704-8

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  • DOI: https://doi.org/10.1007/s12686-012-9704-8

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