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Isolation and characterization of nineteen microsatellite loci for the collector sea urchin, Tripneustes gratilla

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Abstract

Nineteen polymorphic microsatellite loci were developed for the collector sea urchin, Tripneustes gratilla. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Roche 454 technology. The 19 loci were characterized in 24 individuals from the Indonesian archipelago. Observed levels of heterozygosity ranged from 0.21–0.92 with a mean of 12.2 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and 5 loci significantly deviated from Hardy–Weinberg expectations.

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Acknowledgments

We thank the Indonesian government, the Indonesian Institute of Sciences (LIPI) and RISTEK for collection permits, 0085/SIP/FRP/SM/V/2010 and 0133/SIP/FRP/SM/V/2010 under which all collections were made in full accordance with Indonesian laws and regulations. A special thank you is reserved for Mr Onny Nurrahman Marwayana and Ms Hagi Yulia Sugeha. Funding for this work was provided in part by the TOTAL Foundation, University of Hawai‘i Graduate Student Organization, University of Hawai‘i Arts and Sciences Student Research Award, University of Hawai‘i Edmondson Grant, Sigma XI Grants In Aid of Research, The Explorers Club, The Rufford Small Grants Foundation and the University of Hawai‘i NSF-EPSCoRprogram (Investing in Multidisciplinary University Activities, EPS-0903833, J. Gaines, PI) in support of the core functional genomics facility at the Hawai‘i Institute of Marine Biology. This is HIMB contribution # 1500 and The School for Ocean and Earth Science and Technology contribution # 8684.

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Authors and Affiliations

  1. Hawai‘i Institute of Marine Biology, University of Hawai‘i, Mānoa, P.O. Box 1346, Kāneohe, HI, 96744, USA

    Benjamin J. Wainwright & Stephen A. Karl

  2. Research Center for Oceanography, Indonesian Institute of Sciences, Jl. Pasir Putih I, Ancol Timur, Jakarta, 14430, Indonesia

    Irma S. Arlyza

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  1. Benjamin J. Wainwright
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  2. Irma S. Arlyza
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  3. Stephen A. Karl
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Correspondence to Stephen A. Karl.

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Wainwright, B.J., Arlyza, I.S. & Karl, S.A. Isolation and characterization of nineteen microsatellite loci for the collector sea urchin, Tripneustes gratilla . Conservation Genet Resour 4, 963–965 (2012). https://doi.org/10.1007/s12686-012-9683-9

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  • DOI: https://doi.org/10.1007/s12686-012-9683-9

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