Abstract
Species in the genus Bathymodiolus are mytilid mussels found at deep-sea hydrothermal vents and cold seeps. Next-generation sequencing techniques were employed to identify eighteen unlinked polymorphic microsatellite loci for Bathymodiolus manusensis from Manus Basin in the western Pacific. Allele frequencies for eight loci conform to Hardy–Weinberg expectations and observed heterozygosities ranged from 0.04 to 0.90 (mean H O = 0.45, SD = 0.25). Ten of eighteen loci cross-amplified in Bathymodiolus heckerae from Atlantic seeps. Microsatellites developed for Bathymodiolus manusensis are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.
This is a preview of subscription content, log in via an institution to check access.
References
Antao T, Lopes A, Lopes RJ, Beja-Pereira A, Luikart G (2008) LOSITAN: a workbench to detect molecular adaptation based on a Fst-outlier method. BMC Bioinformatics 9:323
Carney SL, Formica MI, Divatia H, Nelson K, Fisher CR, Schaeffer SW (2006) Population structure of the mussel “Bathymodiolus” childressi from Gulf of Mexico hydrocarbon seeps. Deep Sea Res I 53:1061–1072
Daguin C, Jollivet D (2005) Development and cross-amplification of nine polymorphic microsatellite markers in the deep-sea hydrothermal vent polychaete Branchipolynoe seepensis. Mol Ecol Notes 5:780–783
Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinformatics Online 1:47–50
Faircloth L (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Faure B, Jollivet D, Tanguy A, Bonhomme F, Bierne N (2009) Speciation in the deep sea: multi-locus analysis of divergence and gene flow between two hybridizing species of hydrothermal vent mussels. PLoS One 4:e6485
Fusaro AJ, Baco AR, Gerlach G, Shank TM (2008) Development and characterization of 12 microsatellite markers from the deep-sea hydrothermal vent siboglinid Riftia pachyptila. Mol Ecol Resour 8:132–134
Hashimoto J, Furuta M (2007) A new species of Bathymodiolus (Bivalvia: Mytilidae) from hydrothermal vent communities in the Manus Basin, Papau New Guinea. Venus 66:57–68
Kyuno A, Shintaku M, Fujita Y, Matsumoto H, Utsumi M, Watanabe H, Fujiwara Y, Miyazaki J-I (2009) Dispersal and differentiation of deep-sea mussels of the genus Bathymodiolus (Mytilidae, Bathymodiolinae). J Mar Biol. doi:10.1155/2009/625672
Miyazaki J-I, Shintaku M, Kyuno A, Fujiwara Y, Hashimoto J, Iwasaki H (2004) Phylogenetic relationships of deep-sea mussels of the genus Bathymodiolus (Bivalvia: Mytilidae). Mar Biol 144:527–535
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Thaler AD, Zelnio KA, Jones RE, Carlsson J, Van Dover CL, Schultz TF (2010) Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents. Conserv Genet Resour. doi:10.1007/s12686-010-9174-9
Van Dover CL, German CR, Speer KG, Parson LM, Vrijenhoek RC (2002) Evolution and biogeography of deep-sea vent and seep invertebrates. Science 295:1253–1257
van Oosterhout C, Hutchinson W, Willds D, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Won Y-J, Hallam S, O’Mullan GD, Pan IL, Buck KR, Vrijenhoek RC (2003) Environmental acquisition of thiotrophic endosymbionts by deep-sea mussels of the genus Bathymodiolus. Appl Environ Microbiol 69:6785–6792
Zelnio KA, Thaler AD, Jones RE, Saleu W, Schultz TF, Van Dover CL, Carlsson J (2010) Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea: Caridea: Alvinocarididae) from deep-sea hydrothermal vents. Conserv Genet Resour. doi:10.1007/s12686-010-9243-0
Acknowledgments
This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD, JC, and TS and by Duke University. JC acknowledges support from the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme. Samples of Bathymodiolus heckerae were collected with support of the NOAA Ocean Exploration Program through an award to CLVD. Research by AE and P-Y H was supported in part by the Rachel Carson Scholars Program at the Duke University Marine Laboratory. We are grateful to Lisa Bukovnik in the IGSP at Duke University for performing the GS-FLX Titanium sequencing. Samples used in this study were collected on behalf of the people of Papua New Guinea.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Schultz, T.F., Hsing, PY., Eng, A. et al. Characterization of 18 polymorphic microsatellite loci from Bathymodiolus manusensis (Bivalvia, Mytilidae) from deep-sea hydrothermal vents. Conservation Genet Resour 3, 25–27 (2011). https://doi.org/10.1007/s12686-010-9272-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9272-8