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Development of twelve novel microsatellite loci in the European lobster (Homarus gammarus)

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Abstract

We developed twelve novel microsatellite loci primers in the European lobster (Homarus gammarus). All markers were obtained from partial genomic DNA libraries enriched for tetranucleotide repeats and characterized in 48 unrelated individuals from one putative population. The number of alleles ranged from 5 to 13, with an average of 8.3 per locus, and the observed heterozygosity ranged from 0.35 to 0.83 (average 0.69). These microsatellite loci can be used as markers in the assessment of connectivity and genetic structure of exploited lobster populations.

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Acknowledgments

This work was supported by the Norwegian government and EU through the INTERREG III A programme (http://www.imr.no/grensehummer/nb-no). We thank the Swedish Board of Fisheries for lobster samples, and Hanne Sannæs, Anna-Karin Ring and Kate Enersen for technical assistance in the lab. We also thank Mark Todd, Ken Jones, Greg Sadowski and Fred Fernando at GIS for valuable assistance for developing primers and for supervision of the text.

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Authors and Affiliations

  1. Department of Marine Ecology-Tjärnö, University of Gothenburg, 452-96, Strömstad, Sweden

    Carl André

  2. Flødevigen Marine Research Station, Institute of Marine Research, 4817, His, Norway

    Halvor Knutsen

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  1. Carl André
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  2. Halvor Knutsen
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Correspondence to Carl André.

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André, C., Knutsen, H. Development of twelve novel microsatellite loci in the European lobster (Homarus gammarus). Conservation Genet Resour 2 (Suppl 1), 233–236 (2010). https://doi.org/10.1007/s12686-009-9151-3

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  • DOI: https://doi.org/10.1007/s12686-009-9151-3

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