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Supplementation of ascorbic acid and alpha-tocopherol is useful to preventing bone loss linked to oxidative stress in elderly

  • JNHA: Clinical Trials and Aging
  • Published:
The journal of nutrition, health & aging

Abstract

Objective

To determine the effect of ascorbic acid and alpha-tocopherol on oxidative stress and bone mineral density (BMD) in elderly people.

Design

A double-blind, controlled clinical assay was carried out in a sample of 90 elderly subjects divided into three age-paired random groups with 30 subjects in each group. Group Tx0 received placebo, group Tx1 received 500 mg of ascorbic acid and 400 IU of alpha-tocopherol, whereas group Tx2 received 1,000 mg of ascorbic acid and 400 IU of alpha-tocopherol, for a 12-month period.

Measurements

We measured thiobarbituric acid reactive substances (TBARS), total antioxidant status (TAS), superoxide dismutase (SOD), and glutation peroxidase (GPx); BMD was obtained on DXA of hip and spine before and after the 12-month treatment period with supplementation of vitamins C and E.

Results

We found a positive correlation between hip-BMD and SOD (r = 0.298, p <0.05) and GPx (r = 0.214, p <0.05). Also, a significantly lower decrease of LPO (p <0.05) was observed as linked with hip bone loss in the Tx2 group than in the Tx0 group.

Conclusions

Our findings suggest that that administration of 1,000 mg of ascorbic acid together with 400 IU of alpha-tocopherol could be useful in preventing or aiding in the treatment of age-related osteoporosis.

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References

  1. Harman D: Aging: a theory based on free radical and radiation chemistry. J Gerontol 1956; 11: 298–300.

    CAS  PubMed  Google Scholar 

  2. Cheeseman KH, Slater TF: An introduction to free radical biochemistry. Br Med Bull 1993; 49(3):481–493.

    CAS  PubMed  Google Scholar 

  3. Gutteridge JM: Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995;41:1819–1828.

    CAS  PubMed  Google Scholar 

  4. Liehr JC, Roy D: Pro-oxidant and antioxidant effects of estrogens. In: Armstrong D. Free radical and antioxidant protocols. New Jersey: Humana Press, 1998: 425–435.

    Chapter  Google Scholar 

  5. Sies H, Stahl W: Vitamins E and C, beta-carotene, and other carotenoids as antioxidants. Am J Clin Nutr 1995; 62(6 Suppl):1315S–1321S.

    CAS  PubMed  Google Scholar 

  6. Stahl W, Sies H: Antioxidant defense: vitamins E and C and carotenoids. Diabetes. 1997; 46 (Suppl 2):S14–S18.

    CAS  PubMed  Google Scholar 

  7. Valda WB: Free radical, antioxidants and ageing. Med Lab Sci 1992; 49: 299–312.

    Google Scholar 

  8. Halliwell B, Gutteridge JM, Cross CE: Free radicals, antioxidants, and human disease: where are we now?. J Lab Clin Med 1992;119(6):598–620.

    CAS  PubMed  Google Scholar 

  9. Niki E: Action of antioxidants against oxidative stress. Proc Natl Acad Sci 1993; 90: 7915–7922.

    Article  Google Scholar 

  10. Gutteridge JM: Biological origin of free radicals, and mechanisms of antioxidant protection. Chem Biol Interact 1994;91(2–3):133–140.

    Article  CAS  PubMed  Google Scholar 

  11. Faure P, Corticelli P, Richard MJ, Arnaud J, Coudray C, Halimi S, Favier A, Roussel AM: Lipid peroxidation and trace element status in diabetic ketotic patients: Influence of insulin therapy. Clin Chem 1993; 39: 789–793.

    CAS  PubMed  Google Scholar 

  12. Knight JA: Free radicals, antioxidants, aging, and disease. Washington: AACC Press, 1999.

    Google Scholar 

  13. Dequeker J: Bone and ageing. Ann Rheum Dis 1975; 34: 100–115.

    Article  CAS  PubMed  Google Scholar 

  14. Consenso Mexicano de osteoporosis: Asociación Mexicana de Metabolismo Óseo y Mineral. Rev Inv Clin 2001; 53(5): 469–495.

  15. Kleerekoper M: The pathophysiology of osteoporosis. In: Rosen CJ. Osteoporosis. Diagnostic and therapeutic principles. New York: Human Press, 1996: 65–68.

    Google Scholar 

  16. Cumming RG: Calcium intake and bone mass: a quantitative review of the evidence. Calcif Tissue Int 1990; 47: 194–201.

    Article  CAS  PubMed  Google Scholar 

  17. Parra-Cabrera S, Hernandez-Avila M, Tamayo-y-Orozco J, López-Carrillo L, Meneses-González F. Exercise and reproductive factors as predictors of bone density among osteoporotic women in Mexico City. Calcif Tissue Int. 1996; 59(2):89–94.

    Article  CAS  PubMed  Google Scholar 

  18. Felson DT, Zhang Y, Hannan MT, Kannel WB, Kiel DP: Alcohol intake and bone mineral density in elderly men and women. The Framingham Study. Am J Epidemiol 1995; 142:485–492.

    CAS  PubMed  Google Scholar 

  19. Krall EA, Dawson-Hughes B: Smoking and bone loss among postmenopausal woman. J Bone Miner Res 1991; 6: 331–338.

    Article  CAS  PubMed  Google Scholar 

  20. Key LL Jr, Ries WL, Glasscock H, Rodriguiz R, Jaffe H: Osteoclastic superoxide generation: taking control of bone resorption using modulators of superoxide concentrations. Int J Tissue React 1992; 14: 295–298.

    CAS  PubMed  Google Scholar 

  21. Strassheim D, Asehnoune K, Park JS, Kim JY, He Q, Richter D, Mitra S, Arcaroli J, Kuhn K, Abraham E: Modulation of bone marrow-derived neutrophil signaling by H2O2: disparate effects on kinases, NF-kB, and citokine expression. Am J Physiol Cell 2004; 286: C683–C692.

    Article  CAS  Google Scholar 

  22. Hikiji H, Shin WS, Koizumi T, Takato T, Susami T, Koizumi Y, Okai-Matsuo Y, Toyo-Oka T: Peroxynitrite production by TNF-α and IL-1β: implication for suppression of osteoblastic differentiation. Am J Physiol Endocrinol Metab 2000; 278:E1031–1037

    CAS  PubMed  Google Scholar 

  23. Maggio D, Barabani M, Pierandrei M, Polidori MC, Catani M, Mecocci P, Senin U, Pacifici R, Cherubini A: Marked decrease in plasma antioxidants in aged osteoporotic women: results of a cross-sectional study. J Clin Endocrinol Metab. 2003;88:1523–1527.

    Article  CAS  PubMed  Google Scholar 

  24. Bai XC, Lu D, Bai J, Zheng H, Ke ZY, Li XM, Luo SQ: Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB. Biochem Biophys Res Commun 2004; 314: 197–207.

    Article  CAS  PubMed  Google Scholar 

  25. Sánchez-Rodríguez MA, Arellano-Pimentel B, Vargas-Guadarrama LA, Mendoza-Núñez VM: Relación entre la densidad mineral ósea y los niveles de antioxidantes totales en una población de adultos mayores. Arch Geriatr 2002; 4:106–108.

    Google Scholar 

  26. Arjmandi BH, Juma S, Beharka A, Bapna MS, Akhter M, Meydani SN: Vitamin E improves bone quality in the aged but no in the young adult male mice. J Nutr Biochem 2002; 13: 543–549.

    Article  CAS  PubMed  Google Scholar 

  27. Martínez-Maldonado M, Correa-Muñoz E, Mendoza-Núñez VM: Program of active aging in a rural Mexican community: a qualitative approach. BMC Public Health 2007; 7:276.

    Article  Google Scholar 

  28. Altman DG, Schulz KF, Moher D, Egger M, Davidoff F, Elbourne D, Gøtzsche PC, Lang T; CONSORT GROUP (Consolidated Standards of Reporting Trials): The revised CONSORT statement for reporting randomized trials: explanation and elaboration. Ann Intern Med. 2001; 134:663–694.

    CAS  PubMed  Google Scholar 

  29. Jentzsch AM, Bachmann H, Fürst P, Biesalski HK: Improved analysis of malondialdehyde in human body fluids. Free Radic Biol Med 1996; 20:251–256.

    Article  CAS  PubMed  Google Scholar 

  30. Chrischilles EA, Butler CD, Davis CS, Wallace RB: A model of lifetime osteoporosis impact. Arch Intern Med 1991;151:2026–2032.

    Article  CAS  PubMed  Google Scholar 

  31. Cummings SR, Kelsey JL, Nevitt MC, O’Dowd KJ: Epidemiology of osteoporosis and osteoporotic fractures. Epidemiol Rev 1985;7: 178–208.

    CAS  PubMed  Google Scholar 

  32. Reid IR: Therapy of osteoporosis: calcium, vitamin D, and exercise. Am J Med Sci. 1996; 312(6):278–286.

    Article  CAS  PubMed  Google Scholar 

  33. Gennari C: Calcitonin, bone-active isoflavones and vitamin D metabolites. Osteoporos Int 1999;9 (Suppl 2):S81–S90.

    Article  PubMed  Google Scholar 

  34. Khan A: Advances in osteoporosis therapy. 2003 update of practical guidelines. Can Fam Physician 2003; 49:441–447.

    PubMed  Google Scholar 

  35. Hall SL, Greendale GA: The relation of dietary vitamin C intake to bone mineral density: results from the PEPI study. Calcif Tissue Int 1998; 63: 183–189.

    Article  CAS  PubMed  Google Scholar 

  36. Melhus H, Michaëlsson K, Holmberg L, Wolk A, Ljunghall S: Smoking, antioxidant vitamins, and the risk of hip fracture. J Bone Miner Res 1999; 14(1):129–135.

    Article  CAS  PubMed  Google Scholar 

  37. Avitabile M, Campagna NE, Magrì GA, Vinci M, Sciacca G, Alia G, Ferro A: Correlation between serum glutathione reductases and bone densitometry values. Boll Soc Ital Biol Sper 1991; 67: 931–937.

    CAS  PubMed  Google Scholar 

  38. Sánchez-Rodríguez MA, Ruiz-Ramos M, Correa-Muñoz E, Mendoza-Núñez VM: Oxidative stress as a risk factor for osteoporosis in elderly Mexican as characterized by antioxidant enzymes. BMC Musculoskelet Disord 2007; 8:124.

    Article  PubMed  Google Scholar 

  39. Sahni S, Hannan MT, Gagnon D, Blumberg J, Cupples LA, Kiel DP, Tucker KL: High vitamin C intake is associated with lower 4-year bone loss in elderly men. J Nutr 2008; 138:1931–1938.

    CAS  PubMed  Google Scholar 

  40. Pasco JA, Henry MJ, Wilkinson LK, Nicholson GC, Schneider HG, Kotowicz MA: Antioxidant vitamin supplements and markers of bone turnover in a community sample of nonsmoking women. J Womens Health (Larchmt) 2006;15(3):295–300.

    Article  Google Scholar 

  41. Turan B, Can B, Delilbasi E: Selenium combined with vitamin E and vitamin C restores structural alterations of bones in heparin-induced osteoporosis. Clin Rheumatol 2003; 22(6): 432–436.

    Article  PubMed  Google Scholar 

  42. Wolf RL, Cauley JA, Pettinger M, Jackson R, Lacroix A, Leboff MS, Lewis CE, Nevitt MC, Simon JA, Stone KL, Wactawski-Wende J: Lack of a relation between vitamin and mineral antioxidants and bone mineral density: results from the Women’s Health Initiative. Am J Clin Nutr 2005; 82:581–588.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Unidad de Investigación en Gerontología, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México (UNAM), México D.F., México., Batalla 5 de mayo s/n, esq. Fuerte de Loreto, Col. Ejército de Oriente, 09230, México, DF., México

    M. Ruiz-Ramos, A. Cervantes-Sandoval & V. M. Mendoza-Nú`nez

  2. Instituto de Investigaciones Antropológicas, UNAM, México, DF., México

    L. Alberto Vargas

  3. Facultad de Medicina, UNAM, México, DF., México

    T. I. Fortoul Van Der Goes

  4. Guelatao # 66, Col. Ejército de Oriente, 09230, México, DF, México

    V. M. Mendoza-Nú`nez

Authors
  1. M. Ruiz-Ramos
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  2. L. Alberto Vargas
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  3. T. I. Fortoul Van Der Goes
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  4. A. Cervantes-Sandoval
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  5. V. M. Mendoza-Nú`nez
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Correspondence to V. M. Mendoza-Nú`nez.

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Ruiz-Ramos, M., Alberto Vargas, L., Fortoul Van Der Goes, T.I. et al. Supplementation of ascorbic acid and alpha-tocopherol is useful to preventing bone loss linked to oxidative stress in elderly. J Nutr Health Aging 14, 467–472 (2010). https://doi.org/10.1007/s12603-010-0099-5

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  • DOI: https://doi.org/10.1007/s12603-010-0099-5

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