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Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres

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Abstract

The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.

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Acknowledgments

The authors would like to thank the Ministry of Higher Education Malaysia for providing financial assistance during this work through the project, No. RACE 12-002-0002.

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Authors and Affiliations

  1. Kulliyyah of Pharmacy, International Islamic University Malaysia (IIUM), 25200, Kuantan, Malaysia

    Rezaul H. Ansary, Mokhlesur M. Rahman, Hazrina Hadi, Farahidah Mohamed & Abd Almonem Doolaanea

  2. Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, 63000, Cyberjaya, Malaysia

    Mohamed B. Awang

  3. Drug Delivery and Novel Targeting Research Group, Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300, Kuala Lumpur, Malaysia

    Haliza Katas

  4. Kulliyyah of Science, International Islamic University Malaysia, 25200, Kuantan, Malaysia

    Yunus B. Kamaruzzaman

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  1. Rezaul H. Ansary
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Correspondence to Mokhlesur M. Rahman.

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Ansary, R.H., Rahman, M.M., Awang, M.B. et al. Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres. Arch. Pharm. Res. 39, 1242–1256 (2016). https://doi.org/10.1007/s12272-016-0710-3

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  • DOI: https://doi.org/10.1007/s12272-016-0710-3

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