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Culturable bacterial microbiota of Plagiodera versicolora (L.) (Coleoptera: Chrysomelidae) and virulence of the isolated strains

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Abstract

Plagiodera versicolora (Laicharting, 1781) (Coleoptera: Chrysomelidae) is an important forest pest which damages many trees such as willow, poplar, and hazelnut. In order to find new microbes that can be utilized as a possible microbial control agent against this pest, we investigated the culturable bacterial flora of it and tested the isolated bacteria against P. versicolora larvae and adults. We were able to isolate nine bacteria from larvae and adults. The isolates were characterized using a combination of morphological, biochemical, and physiological methods. Additionally, we sequenced the partial sequence of the 16S rRNA gene to verify conventional identification results. Based on characterization studies, the isolates were identified as Staphylococcus sp. Pv1, Rahnella sp. Pv2, Rahnella sp. Pv3, Rahnella sp. Pv4, Rahnella sp. Pv5, Pantoea agglomerans Pv6, Staphylococcus sp. Pv7, Micrococcus luteus Pv8, and Rahnella sp. Pv9. The highest insecticidal activity against larvae and adults was obtained from M. luteus Pv8 with 50 and 40 % mortalities within 10 days after treatment, respectively. Extracellular enzyme activity of the bacterial isolates such as amylase, proteinase, lipase, cellulose, and chitinase was also determined. Consequently, our results show that M. luteus Pv8 might be a good candidate as a possible microbial control agent against P. versicolora and were discussed with respect to biocontrol potential of the bacterial isolates.

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Acknowledgments

I would like to thank Emine Esra Budak and Tugba Ozmen for their technical assistance.

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdoğan University, Rize-53100, Turkey

    Meryem Demirci, Elif Sevim & Ali Sevim

  2. Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, 61080, Turkey

    İsmail Demir

  3. Ahi Evran University, Faculty of Engineering and Architecture, Environmental Engineering, Kırşehir-40100, Turkey

    Elif Sevim & Ali Sevim

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  1. Meryem Demirci
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  2. Elif Sevim
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Correspondence to Ali Sevim.

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Demirci, M., Sevim, E., Demir, İ. et al. Culturable bacterial microbiota of Plagiodera versicolora (L.) (Coleoptera: Chrysomelidae) and virulence of the isolated strains. Folia Microbiol 58, 201–210 (2013). https://doi.org/10.1007/s12223-012-0199-1

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