Abstract
High-fidelity replication guarantees the stable inheritance of genetic information stored in the DNA of living organisms. The minichromosome maintenance (MCM) complex functions as replicative DNA-unwinding helicase and has been identified as one key player in the replication process of archea and eukarya. Despite the availability of considerable structural information on archeal MCMs, such information was missing for their C-terminal domain. In order to obtain more detailed structural information, we assigned the NMR chemical shifts for backbone and side chain nuclei for the MCM C-terminal winged helix domains of the archeal species Methanothermobacter thermautrophicus and Sulfolobus solfataricus.
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Acknowledgments
We are grateful to Dr. K.-H. Gührs for the mass spectrometric analyses of SsoE605. CW was supported by the Leibniz Graduate School on Ageing and Age-Related Diseases (LGSA). The FLI is a member of Science Association "Gottfried Wilhelm Leibniz (WGL)" and is financially supported by the Federal Government of Germany and the State of Thuringia.
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Wiedemann, C., Ohlenschläger, O., Medagli, B. et al. 1H, 15N and 13C chemical shift assignments for the winged helix domains of two archeal MCM C-termini. Biomol NMR Assign 8, 357–360 (2014). https://doi.org/10.1007/s12104-013-9516-0
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DOI: https://doi.org/10.1007/s12104-013-9516-0