Abstract
In this paper, we report the dose-dependent antioxidant activity and DNA protective effects of zingerone. At 500 μg/mL, the DPPH radical scavenging activity of zingerone and ascorbic acid as a standard was found to be 86.7 and 94.2 % respectively. At the same concentration, zingerone also showed significant reducing power (absorbance 0.471) compared to that of ascorbic acid (absorbance 0.394). The in vitro toxicity of stannous chloride (SnCl2) was evaluated using genomic and plasmid DNA. SnCl2-induced degradation of genomic DNA was found to occur at a concentration of 0.8 mM onwards with complete degradation at 1.02 mM and above. In the case of plasmid DNA, conversion of supercoiled DNA into the open circular form indicative of DNA nicking activity was observed at a concentration of 0.2 mM onwards; complete conversion was observed at a concentration of 1.02 mM and above. Zingerone was found to confer protection against SnCl2-induced oxidative damage to genomic and plasmid DNA at concentrations of 500 and 750 μg/mL onwards, respectively. This protective effect was further confirmed in the presence of UV/H2O2-a known reactive oxygen species (ROS) generating system-wherein protection by zingerone against ROS-mediated DNA damage was observed at a concentration of 250 μg/mL onwards in a dose-dependent manner. This study clearly indicated the in vitro DNA protective property of zingerone against SnCl2-induced, ROS-mediated DNA damage.
References
El-Demerdash F, Yousef M, Zoheir MA (2005) Stannous chloride induces alterations in enzyme activities, lipid peroxidation and histopathology in male rabbit: antioxidant role of vitamin C. Food Chem Toxicol 43:1743–1752
Hattori T, Maehashi H (1994) Augmentation of calcium influx by stannous chloride at mouse motor nerve terminals. Res Commun Chem Pathol Pharmacol 84:253–256
Yousef MI (2005) Protective role of ascorbic acid to enhance reproductive performance of male rabbits treated with stannous chloride. Toxicology 207:81–89
Viau CM, Cardone JM, Guecheva TN, M-Lc Y, Dias JF, Pungartnik C, Brendel M, Saffi J, JoA H (2011) Enhanced resistance of yeast mutants deficient in low-affinity iron and zinc transporters to stannous-induced toxicity. Chemosphere 86:477–484
El-Makawy AI, Girgis SM, Khalil WK (2008) Developmental and genetic toxicity of stannous chloride in mouse dams and fetuses. Mutat Res-Gen Tox En 657:105–110
De Mattos JC, Lage C, FvJ D, Moraes MO, Nunes AP, Bezerra RJ, Faria MVC, Leitão AC, Caldeira-de-Araujo A (2005) Interaction of stannous chloride leads to alteration in DNA, triphosphate nucleotides and isolated bases. Mol Cell Biochem 280:173–179
Cabral RE, Leitão AC, Lage C, Caldeira-de-Arauo A, Bernardo-Filho M, Dantas FJ, Cabral-Neto JB (1998) Mutational potentiality of stannous chloride: an important reducing agent in the Tc-99m-radiopharmaceuticals. Mutat Res-DNA Repair 408:129–135
Viau C, Guecheva TN, Sousa F, Pungartnik C, Brendel M, Saffi J, JoAPg H (2009) SnCl2-induced DNA damage and repair inhibition of MMS-caused lesions in V79 Chinese hamster fibroblasts. Arch Toxicol 83:769–775
Assis MLB, Caceres MR, De Mattos JC, Caldeira-de-Arauo A, Bernardo-Filho M (1998) Cellular inactivation induced by a radiopharmaceutical kit: role of stannous chloride. Toxicol Lett 99:199–205
FvJ D, Moraes MO, de Mattos JC, Bezerra RJ, Carvalho EF, Mr BF, Caldeira de Arauo A (1999) Stannous chloride mediates single strand breaks in plasmid DNA through reactive oxygen species formation. Toxicol Lett 110:129–136
Silva C, Oliveira M, Melo S, Dantas F, De Mattos J, Bezerra R, Caldeira-de-Araujo A, Duatti A, Bernardo-Filho M (2002) Biological effects of stannous chloride, a substance that can produce stimulation or depression of the central nervous system. Brain Res Bull 59:213–216
Beckman KB, Ames BN (1997) Oxidative decay of DNA. J Biol Chem 272:19633–19636
Henle ES, Linn S (1997) Formation, prevention, and repair of DNA damage by iron/hydrogen peroxide. J Biol Chem 272:19095–19098
Valko M, Rhodes C, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–40
Gu L, Wu T, Wang Z (2009) TLC bioautography-guided isolation of antioxidants from fruit of Perilla frutescens var. acuta. LWT-Food Sci Technol 42:131–136
Hu W, Yu L, Wang M-H (2011) Antioxidant and antiproliferative properties of water extract from Mahonia bealei (Fort.) Carr. leaves. Food Chem Toxicol 49:799–806
Zhu K-X, Lian C-X, Guo X-N, Peng W, Zhou H-M (2011) Antioxidant activities and total phenolic contents of various extracts from defatted wheat germ. Food Chem 126:1122–1126
Shukla Y, Singh M (2007) Cancer preventive properties of ginger: a brief review. Food Chem Toxicol 45:683–690
Parthasarathy VA, Chempakam B, Zachariah TJ (2008) Chemistry of spices. CABI, Oxfordshire
Rao BN, Satish Rao B, Kiran Aithal B, Sunil Kumar M (2009) Radiomodifying and anticlastogenic effect of Zingerone on Swiss albino mice exposed to whole body gamma radiation. Mutat Res-Gen Tox En 677:33–41
Rao BN, Archana PR, Aithal BK, Rao BSS (2011) Protective effect of zingerone, a dietary compound against radiation induced genetic damage and apoptosis in human lymphocytes. Eur J Pharmacol 657:59–66
Kabuto H, Yamanushi TT (2011) Effects of zingerone [4-(4-hydroxy-3-methoxyphenyl)-2-butanone] and eugenol [2-methoxy-4-(2-propenyl) phenol] on the pathological progress in the 6-hydroxydopamine-induced Parkinson’s disease mouse model. Neurochem Res 36:2244–2249
Chen I-N, Chang C-C, Ng C-C, Wang C-Y, Shyu Y-T, Chang T-L (2008) Antioxidant and antimicrobial activity of Zingiberaceae plants in Taiwan. Plant Food Hum Nutr 63:15–20
Dong S, Hwang HM, Shi X, Holloway L, Yu H (2000) UVA-induced DNA single-strand cleavage by 1-hydroxypyrene and formation of covalent adducts between DNA and 1-hydroxypyrene. Chem ResToxicol 13:585–593
Russo A, Izzo AA, Borrelli F, Renis M, Vanella A (2003) Free radical scavenging capacity and protective effect of Bacopa monniera L. on DNA damage. Phytotherapy Res 17:870–875
Croft KD (1999) Antioxidant effects of plant phenolic compounds. In: Basu TK, Temple NJ, Garg ML (eds) Antioxidants in human health and disease. CABI, New York, pp 112–115
Ratnam DV, Ankola D, Bhardwaj V, Sahana DK, Kumar M (2006) Role of antioxidants in prophylaxis and therapy: a pharmaceutical perspective. J Control Release 113:189–207
Heaton PR, Reed CF, Mann SJ, Ransley R, Stevenson J, Charlton CJ, Smith BH, Harper EJ, Rawlings JM (2002) Role of dietary antioxidants to protect against DNA damage in adult dogs. J Nutr 132:1720S–1724S
Shin S-G, Kim JY, Chung HY, Jeong J-C (2005) Zingerone as an antioxidant against peroxynitrite. J Agric Food Chem 53:7617–7622
Rao BN, Rao BS (2010) Antagonistic effects of Zingerone, a phenolic alkanone against radiation-induced cytotoxicity, genotoxicity, apoptosis and oxidative stress in Chinese hamster lung fibroblast cells growing in vitro. Mutagenesis 25:577–587
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Rajan, I., Narayanan, N., Rabindran, R. et al. Zingerone Protects Against Stannous Chloride-Induced and Hydrogen Peroxide-Induced Oxidative DNA Damage In Vitro. Biol Trace Elem Res 155, 455–459 (2013). https://doi.org/10.1007/s12011-013-9801-x
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DOI: https://doi.org/10.1007/s12011-013-9801-x